PCR Identification of Pathogenic Agrobacteria Detected in Vineyards of Krasnodar Krai Based on the Type of Ti Plasmids

Makarkina, Marina Victorovna; Ilnitskaya, Elena Tarasovna; Токмаков, С. В.

doi:10.3103/s0096392519010048

Cited by 1 publication

(1 citation statement)

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“…The morphological, biochemical and physiological properties showed that the test isolates resembled agrobacteria based on most characteristics. In recent decades, methods based on PCR have been used for the detection of Agrobacterium strains (Makarkina et al 2019;Cubero et al 1999). Based on Ti plasmid several primer pairs such as virD2A/virD2C have been designed and reported for the molecular detection and identification of tumorigenic members of Rhizobiaceae.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Agrobacterium radiobacter, a new pathogen of pistachio

Basavand

Khodaygan

Moradi

et al. 2022

Australasian Plant Pathol.

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Crown and stem gall caused by agrobacterial species is one of the most devastating diseases on different crops worldwide. In spring 2018, an alert concerning a disease forming galls on seedlings of pistachio in nurseries was received from Rafsanjan county, Kerman province, Iran. After isolation of bacteria from the galls by using conventional bacteriological methods, bacterial colonies were purified and selected for further analyses including biochemical, pathogenicity and molecular tests. Pathogenicity tests of all isolates on test plants and carrot discs were evaluated positive. Based on the results of biochemical and pathogenicity tests, supported by molecular phylogenies of housekeeping and pathogenicity genes, the isolates were identified as Agrobacterium radiobacter. Since agrobacteria could be easily spread through seedlings and agricultural materials between different areas, the identified Agrobacterium strains could be considered as a latent threat to the pistachio nurseries of Iran. The present study is the first report on the occurrence of crown and stem gall on pistachio caused by A. radiobacter worldwide.

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Section: Discussionmentioning

confidence: 99%