PCR identification of genes of resistance to black rot in white cabbage using SSR-markers

Abstract

The present study revealed the polymorphism of SSR loci by the resistance of white cabbage to black rot in contrasting isogenic samples of white cabbage. 2 informative SSR markers were selected: Ol10-C01 and Ol11-H06 for ranking breeding samples based on resistance to Xanthomonas campestris pv. campestris Dows. The microsatellite marker Ol10-C01 was tested on breeding samples and reveals polymorphism between them; therefore, it can be recommended for practical breeding for programs to develop black rot-resistant hybrids of cabbage, which will solve the problem of import substitution and healthy food (environmentally friendly products, grown without the use of means of chemical protection).

Molecular marking in breeding Brassica oleracea L. for resistance to Xanthomonas campestris pv. campestris

“…A total of 30 microsatellite markers localized in different chromosomes were tested during DNA analysis of plant forms contrasting in resistance to black rot, 3 of which (Ol10-C01, Ol11-H06, BoESSR 726) revealed polymorphism in the studied samples. Allelic diversity at the Ol10-C01 and Ol11-H06 loci was demonstrated in the previous work [15]. The polymorphism at the BoESSR 726 locus can be seen from Figure 1.…”

“…From the presented electrophoregram, it can be seen that the bends in resistant and susceptible white cabbage samples are identical, therefore, these markers are not suitable for further breeding work on the basis of resistance to Xanthomonas campestris. At the next stage, the microsatellite markers Ol10-C01 and Ol11-H06, which revealed polymorphism according to the results of our earlier study [15], were tested on breeding samples of white cabbage (F2 plants of the hybrid combination 269-Yas12p-2 x Pi714) to check their co-inheritance with the resistance to Xanthomonas campestris, which is presented in Figures 3 and 4.…”