PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

Ivanescu, Maria-Larisa; Acatrinei, Dumitru; Pavel, Ionut; Șuleșco, Tatiana; Miron, Liviu

doi:10.1515/ap-2015-0040

Cited by 9 publications

(4 citation statements)

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“…labranchiae in southern Italy and in new, hilly areas northeastward, indicating a potential expansion of the species area to new ecological settings [ 90 , 91 ]. Anopheles labranchiae was found for the first time in Romania [ 92 ] as larvae in a natural pond. The molecular analysis of collected specimens showed a similarity of 96% to specimens from Italy, suggesting that this species was imported from that country, taking into consideration that many inhabitants of the area worked in Italy and came back to Romania during the summer.…”

Section: Updates On the Occurrence And Bionomics Of The Potential Vec...mentioning

confidence: 99%

Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000–2021)

et al. 2022

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Despite the eradication of malaria across most European countries in the 1960s and 1970s, the anopheline vectors are still present. Most of the malaria cases that have been reported in Europe up to the present time have been infections acquired in endemic areas by travelers. However, the possibility of acquiring malaria by locally infected mosquitoes has been poorly investigated in Europe, despite autochthonous malaria cases having been occasionally reported in several European countries. Here we present an update on the occurrence of potential malaria vector species in Europe. Adopting a systematic review approach, we selected 288 papers published between 2000 and 2021 for inclusion in the review based on retrieval of accurate information on the following Anopheles species: An. atroparvus, An. hyrcanus sensu lato (s.l.), An. labranchiae, An. maculipennis sensu stricto (s.s.), An. messeae/daciae, An. sacharovi, An. superpictus and An. plumbeus. The distribution of these potential vector species across Europe is critically reviewed in relation to areas of major presence and principal bionomic features, including vector competence to Plasmodium. Additional information, such as geographical details, sampling approaches and species identification methods, are also reported. We compare the information on each species extracted from the most recent studies to comparable information reported from studies published in the early 2000s, with particular reference to the role of each species in malaria transmission before eradication. The picture that emerges from this review is that potential vector species are still widespread in Europe, with the largest diversity in the Mediterranean area, Italy in particular. Despite information on their vectorial capacity being fragmentary, the information retrieved suggests a re-definition of the relative importance of potential vector species, indicating An. hyrcanus s.l., An. labranchiae, An. plumbeus and An. sacharovi as potential vectors of higher importance, while An. messeae/daciae and An. maculipennis s.s. can be considered to be moderately important species. In contrast, An. atroparvus and An. superpictus should be considered as vectors of lower importance, particularly in relation to their low anthropophily. The presence of gaps in current knowledge of vectorial systems in Europe becomes evident in this review, not only in terms of vector competence but also in the definition of sampling approaches, highlighting the need for further research to adopt the appropriate surveillance system for each species. Graphical Abstract

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Section: Updates On the Occurrence And Bionomics Of The Potential Vec...mentioning

confidence: 99%

Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000–2021)

et al. 2022

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“…Tropical and subtropical climates are the most geographically favourable environments for mosquitoes to thrive and reproduce, making them a significant threat for mosquito-borne illnesses in these regions. Nevertheless, the occurrence of climate change, together with the surge in human travel and migration seen in recent times, has led to the dissemination of these illnesses to regions where they were previously absent [1][2][3][4] . Aedes aegypti poses a significant public health threat since it is very effective in transmitting diseases such as malaria, dengue, Chikungunya, Zika, and other arboviruses [5,6] .…”

Section: Introductionmentioning

confidence: 99%

The implications for vector control of the feeding preference of female Aedes aegypti mosquitoes for different types of human blood and how it affects their fertility

Sadik,

Allam,

Raj

et al. 2024

Int. J. Mosq. Res.

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The aim of the present was to identify the preferential feeding of Ae. aegypti on human ABO blood groups and their impacts on fecundity. Methods: Laboratory reared female Ae. aegypti mosquitoes were exposed to all four blood groups at once in separate membrane feeders. After feeding, DNA of blood in mosquitoes was extracted and identified using ABO genotyping PCR. Fecundity was determined by the mean number of eggs in an egg batch. Results: Among 410 mosquitoes allowed for feeding, 200 individual females were identified as blood fed mosquitoes by amplifying the 334bp fragment in the human DNA detection PCR. In the ABO genotyping PCR, blood groups A (n= 36), B (n=38), AB (n=50) and O (n=61) were detected. Additionally, 15 samples were detected having consumed multiple blood meals. The mean numbers of eggs laid per females were analyzed using one-way ANOVA test with the 0.05 significance level. There was no significant difference in fecundity for different blood groups. Conclusion:The discovery of the underlying mechanism responsible for the preference of Ae. aegypti mosquitoes for blood group O has resulted in the development of a novel trap that attracts adult mosquitoes. This trap shows promise in combating the growing problem of pesticide resistance. Recent research has shown that persons with blood type O are more susceptible to contracting vector-borne illnesses transmitted by the Ae. aegypti mosquito.

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“…These limitations have led to the development and incorporation of molecular tools that facilitate and speed up the identi cation of vectors in areas of high diversity. Multiplex PCR, allele-speci c PCR, and sequencing have been successfully used to diagnose Anopheles species in Malaysia [16], Germany [17], Korea [18], Romania [19], India [20], Ethiopia [21], Sri Lanka [22], Indonesia [23], Papua New Guinea [24] and several other countries [25][26][27][28][29][30]. Other authors have opted for an approach based on digestion with restriction enzymes of DNA sequences such as ITS2, D3, COI, or COII [20,[31][32][33][34][35][36][37][38]].…”

Section: Introductionmentioning

confidence: 99%

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

Escobar

Pérez

Ortiz

et al. 2022

Preprint

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Background Vector populations are a key target for malaria control and elimination. In Honduras there are at least 12 reported anopheline species, however, the definitive number of species remains uncertain. Due to the inherent limitations of morphological identification of Anophelesspecies, molecular approaches have been developed to provide accurate identification and robust surveillance of local malaria vectors. The aim of this study was to design and assess three PCR-RFLP assays to identify anopheline species in Honduras. Methods Mosquitoes captured between 2018 and 2022 in seven malaria-endemic and non-endemic departments in Honduras were analysed. The ITS2 ribosomal region and three restriction enzyme-based assays were evaluated in silico and experimentally. Results A total of 132 sequences from 12 anopheline species were analysed. The ITS2 marker showed length polymorphisms that generated products between 388 bp and 592 bp and no relevant intraspecies polymorphisms were found. Furthermore, the three PCR-RFLP assays were able to differentiate eleven species with sufficient precision and resolution. Conclusion The ITS2 region showed to be a useful molecular marker for identifying local Anophelesspecies. In addition, the PCR-RFLP assays evaluated here proved to be capable of discriminating most of the anopheline species present in Honduras. These methods provide alternatives to improve entomological surveillance of Anophelesin Honduras and other Mesoamerican countries.

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PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

Cited by 9 publications

References 4 publications

Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000–2021)

Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000–2021)

The implications for vector control of the feeding preference of female Aedes aegypti mosquitoes for different types of human blood and how it affects their fertility

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

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