PCR identification of class I major histocompatibility complex genes transcribed in mouse blastocyst and placenta

Landel, Carlisle P.; Stabley, Deborah L.; Bundesen, Liza Q.

doi:10.1016/s0165-0378(96)01013-3

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…All results reported are averages from 3 independent experiments in at least 2 different lines of each strain to exclude possible insertion position effects. Endogenous MHC class I levels were measured the same way using published primers (44).…”

Section: Methodsmentioning

confidence: 99%

Major Histocompatibility Complex Class I Core Promoter Elements Are Not Essential for Transcription in vivo

Barbash

Weissman

Campbell

et al. 2013

Molecular and Cellular Biology

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The role of core promoter elements in regulating transcription initiation is largely unknown for genes subject to complex regulation. Major histocompatibility complex class I genes are ubiquitously expressed and governed by tissue-specific and hormonal signals. Transcription initiates at multiple sites within the core promoter, which contains elements homologous to the canonical elements CCAAT, TATAA, Sp1 binding site (Sp1BS), and Initiator (Inr). To determine their functions, expression of class I transgenes with individually mutated elements was assessed. Surprisingly, all mutant promoters supported transcription. However, each mutated core promoter element had a distinct effect on expression: CAAT box mutations modulated constitutive expression in nonlymphoid tissues, whereas TATAA-like element mutations dysregulated transcription in lymphoid tissues. Inr mutations aberrantly elevated expression. Sp1BS element mutations resulted in variegated transgene expression. RNA polymerase II binding and histone H3K4me3 patterns correlated with transgene expression; H3K9me3 marks partially correlated. Whereas the wild-type, TATAA-like, and CAAT mutant promoters were activated by gamma interferon, the Sp1 and Inr mutants were repressed, implicating these elements in regulation of hormonal responses. These results lead to the surprising conclusion that no single element is required for promoter activity. Rather, each plays a distinct role in promoter activity, chromatin structure, tissue-specific expression, and extracellular signaling.

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Section: Methodsmentioning

confidence: 99%

Major Histocompatibility Complex Class I Core Promoter Elements Are Not Essential for Transcription in vivo

Barbash

Weissman

Campbell

et al. 2013

Molecular and Cellular Biology

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“…All results reported are the average of 2-3 independent experiments in lines derived from different founders, to exclude possible insertion position effects. Endogenous MHC class I levels were determined the same way using published primers (Landel et al 1997).…”

Section: Mnase Hypersensitivity and Nucleosome Occupancymentioning

confidence: 99%

CCAAT Promoter element regulates transgenerational expression of the MHC class I gene

Weissman,

Kotekar,

Barbash

et al. 2024

Chromosoma

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Transgenerational gene expression depends on both underlying DNA sequences and epigenetic modifications. The latter, which can result in transmission of variegated gene expression patterns across multiple generations without DNA alterations, has been termed epigenetic inheritance and has been documented in plants, worms, flies and mammals. Whereas transcription factors binding to cognate DNA sequence elements regulate gene expression, the molecular basis for epigenetic inheritance has been linked to histone and DNA modifications and non-coding RNA. Here we report that mutation of the CCAAT box promoter element abrogates NF-Y binding and disrupts the stable transgenerational expression of an MHC class I transgene. Transgenic mice with a mutated CCAAT box in the MHC class I transgene display variegated expression of the transgene among littermates and progeny in multiple independently derived transgenic lines. After 4 generations, CCAAT mutant transgenic lines derived from a single founder stably displayed distinct patterns of expression. Histone modifications and RNA polymerase II binding correlate with expression of CCAAT mutant transgenic lines, whereas DNA methylation and nucleosome occupancy do not. Mutation of the CCAAT box also results in changes to CTCF binding and DNA looping patterns across the transgene that correlate with expression status. These studies identify the CCAAT promoter element as a regulator of stable transgenerational gene expression such that mutation of the CCAAT box results in variegated transgenerational inheritance. Considering that the CCAAT box is present in 30% of eukaryotic promoters, this study provides insights into how fidelity of gene expression patterns is maintained through multiple generations.

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Procoagulants in fetus rejection: the role of the OX-2 (CD200) tolerance signal

Clark

Levy

et al. 2001

Seminars in Immunology

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PCR identification of class I major histocompatibility complex genes transcribed in mouse blastocyst and placenta

Cited by 6 publications

References 28 publications

Major Histocompatibility Complex Class I Core Promoter Elements Are Not Essential for Transcription in vivo

Major Histocompatibility Complex Class I Core Promoter Elements Are Not Essential for Transcription in vivo

CCAAT Promoter element regulates transgenerational expression of the MHC class I gene

Procoagulants in fetus rejection: the role of the OX-2 (CD200) tolerance signal

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