PCR-free paper-based nanobiosensing platform for visual detection of telomerase activity via gold enhancement

Mahmoudi, Tohid; Tazehkand, Abbas Pirpour; Pourhassan‐Moghaddam, Mohammad; Alizadeh-Ghodsi, Mohammadreza; Ding, Lin; Baradaran, Behzad; Bazaz, Sajad Razavi; Jin, Dayong; Warkiani, Majid Ebrahimi

doi:10.1016/j.microc.2020.104594

Cited by 10 publications

(10 citation statements)

References 45 publications

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“…Visual detection of LFIA results suffers from some limitations: firstly, the user's eyesight impacts on the reading of the TZ, and can become challenging, especially at low concentrations of analyte; secondly, different environmental illumination conditions affect the interpretation of colors and results [24,25]. However, the imaging box developed herein provides constant lightning conditions, and the smartphone quantification platform provides quantitative results in a facile way, preventing the user's eyesight error.…”

Section: Analytical Performance Of the Developed Smartphone-based Lfia Kitmentioning

confidence: 99%

On-Site Detection of Carcinoembryonic Antigen in Human Serum

et al. 2021

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Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.

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Section: Analytical Performance Of the Developed Smartphone-based Lfia Kitmentioning

confidence: 99%

On-Site Detection of Carcinoembryonic Antigen in Human Serum

et al. 2021

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“…However, the detection sensitivity in conventional paper-based devices is relatively low because they suffer from limitations on the physical flow behavior and the time delay of the reaction. The use of metal and metal oxide nanoparticles [ 123 , 124 ], biological catalysts [ 125 ], and chitosan [ 126 ] to modify the paper’s surface has been proposed to enhance the detection signals from μPADs used for automated and multiplexed colorimetric analyses. In addition, the design of μPADs as multilayers and origami platforms can significantly improve the colorimetric signal for highly sensitive detection.…”

Section: Detection Techniquesmentioning

confidence: 99%

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

et al. 2020

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Microfluidic paper-based analytical devices (µPADs) have become promising tools offering various analytical applications for chemical and biological assays at the point-of-care (POC). Compared to traditional microfluidic devices, µPADs offer notable advantages; they are cost-effective, easily fabricated, disposable, and portable. Because of our better understanding and advanced engineering of µPADs, multistep assays, high detection sensitivity, and rapid result readout have become possible, and recently developed µPADs have gained extensive interest in parallel analyses to detect biomarkers of interest. In this review, we focus on recent developments in order to achieve µPADs with high-throughput capability. We discuss existing fabrication techniques and designs, and we introduce and discuss current detection methods and their applications to multiplexed detection assays in relation to clinical diagnosis, drug analysis and screening, environmental monitoring, and food and beverage quality control. A summary with future perspectives for µPADs is also presented.

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“…More importantly, it has been demonstrated to potentially fulfill the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end users) proposed by the World Health Organization (WHO) for the development of POCT applications in areas with limited resources ( Hu et al, 2014 ; Smith et al, 2018 ). Exploiting the rapid growth and recent advances in paper-based technologies for POCT, especially in nucleic acids and immunoassays ( Choi et al, 2017 ; Zhu et al, 2019 ; Lee et al, 2020 ; Mahmoudi et al, 2020 ), this research deployed a novel and easy-to-use technique for sample preparation of exosomal miRNA as biomarker for liquid biopsy by designing a paper-based system, including paper-based immunoaffinity and paper-based silica devices, to capture exosomes derived from HCT116 cells cultured in varied microenvironments and nucleic acids carried by the harvested exosomes. The cells were initially observed by microscopy and the culture media undergoing an ultrafiltration step to concentrate the exosomes and subsequently analyzed by qNano.…”

Section: Introductionmentioning

confidence: 99%

Paper-Based Devices for Capturing Exosomes and Exosomal Nucleic Acids From Biological Samples

Lai

Lee

et al. 2022

Front. Bioeng. Biotechnol.

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Exosomes, nanovesicles derived from cells, contain a variety of biomolecules that can be considered biomarkers for disease diagnosis, including microRNAs (miRNAs). Given knowledge and demand, inexpensive, robust, and easy-to-use tools that are compatible with downstream nucleic acid detection should be developed to replace traditional methodologies for point-of-care testing (POCT) applications. This study deploys a paper-based extraction kit for exosome and exosomal miRNA analytical system with some quantifying methods to serve as an easy sample preparation for a possible POCT process. Exosomes concentrated from HCT116 cell cultures were arrested on paper-based immunoaffinity devices, which were produced by immobilizing anti-CD63 antibodies on Whatman filter paper, before being subjected to paper-based silica devices for nucleic acids to be trapped by silica nanoparticles adsorbed onto Whatman filter paper. Concentrations of captured exosomes were quantified by enzyme-linked immunosorbent assay (ELISA), demonstrating that paper-based immunoaffinity devices succeeded in capturing and determining exosome levels from cells cultured in both neutral and acidic microenvironments, whereas microRNA 21 (miR-21), a biomarker for various types of cancers and among the nucleic acids absorbed onto the silica devices, was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) to prove that paper-based silica devices were capable of trapping exosomal nucleic acids. The developed paper-based kit and the devised procedure was successfully exploited to isolate exosomes and exosomal nucleic acids from different biological samples (platelet-poor plasma and lesion fluid) as clinical applications.

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PCR-free paper-based nanobiosensing platform for visual detection of telomerase activity via gold enhancement

Cited by 10 publications

References 45 publications

On-Site Detection of Carcinoembryonic Antigen in Human Serum

On-Site Detection of Carcinoembryonic Antigen in Human Serum

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

Paper-Based Devices for Capturing Exosomes and Exosomal Nucleic Acids From Biological Samples

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