PCR–ELISA detection of estrogen receptor β mRNA expression and plasma vitellogenin induction in juvenile sole (Solea solea) exposed to waterborne 4-nonylphenol

Palermo, Francesco Alessandro; Cocci, Paolo; Angeletti, Mauro; Polzonetti-Magni, A.M.; Mosconi, Gilberto

doi:10.1016/j.chemosphere.2011.10.058

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…In environmental studies, expression of hepatic VTG is not typically detected in brown bullhead even when collected from areas where AHTN is known to be ubiquitous and other endocrine disrupting compounds have been detected. Generally, ERβ differs from ERα in its response to both natural and synthetic estrogens (Paech et al 1997; however, induction of ERβ in response to environmental estrogens has been identified (Palermo et al 2012). Though induction of VTG did not occur in the present study, the induction of both estrogen receptors further suggests AHTN as an endocrine disruptor in aquatic environments.…”

Section: Gene Expressioncontrasting

confidence: 59%

Development and Application of Quantitative Gene Expression Assays in Wild-Caught, Non-Model Fish Species

Hahn¹

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The Great Lakes Basin is the most significant freshwater resource in North America. It is a central location for diverse anthropogenic enterprises and consequently has been heavily impacted by the release of toxic substances. Environmental studies increasingly identify the presence of both contaminants of emerging concern (CECs) and legacy contaminants in aquatic environments; however, the biological effects of these compounds on resident fishes remain largely unknown. To address this issue, the U.S. Fish and Wildlife Service Environmental Contaminants Programs initiated a multiagency, collaborative study which included chemical analysis of water and benthic sediment, assessment of caged fathead minnows and assessment of wild fish health using a suite of biological endpoints. In an effort to accurately and efficiently assess the ecological risks of complex chemical mixtures through the integration and synthesis of data from many levels of biological organization, sequence databases for non-model, resident fish species were required. This dissertation contains chapters that describe; 1.) The development of partial transcriptomes for three wild-caught, resident fish species 2.) The utilization of these sequence databases in the development of quantitative gene expression assays 3.) The application of these assays in both laboratory and environmental analyses and 4.) The use of transcriptome sequence databases in the identification of a novel virus. iii DEDICATION For my Dad. iv ACKNOWLEDGMENTS I would like to thank the Great Lakes Restoration Initiative for funding of this research. I would like to gratefully acknowledge my committee, Vicki Blazer, Patricia Mazik, Luke Iwanowicz, Amy Welsh and Kyle Hartman for their time and guidance throughout my time as a graduate student. Many people at the Leetown Science Center have helped make this research possible.

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Section: Gene Expressioncontrasting

confidence: 59%

Development and Application of Quantitative Gene Expression Assays in Wild-Caught, Non-Model Fish Species

Hahn¹

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“…Perhaps the idea of investigating the possible risk associated with exposure to these contaminants is still burgeoning in these regions or lack of highly sensitive instruments to determine the levels of these contaminants may be the contributing factors to the scarcity of data. Although scientific evaluation of the risk associated with these contaminants in the natural environment have been criticized by some scholars to be a waste of time due to their extremely low levels [71] visible adverse effects resulting in hormonal and other sexual disorders have been observed and reported in some vertebrate and non-vertebrate species which were scientifically traced to these contaminants [24][25][26]. Even though direct link with human health is yet to be established, it may be possible that the human health effects is cumulative and may manifest over time in areas with large influx and usage of these chemicals with little or no regulation or remediation strategies.…”

Section: Analysis and Environmental Concentrationsmentioning

confidence: 99%

“…Adverse effects of some of these compounds have been observed in aquatic organisms. Several reproductive anomalies have been reported in vertebrate and non-vertebrate aquatic species due to exposure to endocrine disrupting compounds (EDC's) [24][25][26]. Also, EDC's have been recently matched with the ability to interfere with thyroid function or lipid signalling and consequently produce metabolic disruption or incidence of metabolic diseases such as obesity and diabetes, through the activation of peroxisome proliferator activated receptors (PPARs) [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Review of Sources, Fate, Analytical Challenges and Regulatory Status of Emerging Organic Contaminants in Aquatic Environments in Selected African Countries

2019

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Emerging contaminants (ECs) have received great attention in developed countries and as such some of them have received priority screening and regulatory guidelines proposed. However, due to the requirements of highly sensitive and sophisticated analytical equipment, research on this subject in developing countries in Africa are very scarce. We carried out a review of published data from southern and western African region in order to: (1) highlight the environmental concentrations of ECs in West and Southern Africa and bring to light the analytical challenges in order to elicit discussions on solutions, (2) analyse the state of science regarding ECs in African environment and summarise available data for comparative analysis. Generally, the study reveals that in spite of the scanty data reported, levels of ECs are in the upper range of global concentrations. Monitoring data are increasing yearly, although at a very slow rate. Environmentally relevant levels reported are high enough to cause potential risks. Priority monitoring of phenolic endocrine disrupting compounds, phthalate esters, triclosan, triclocarban and some pharmaceutical active compounds (PhACs) like ibuprofen are recommended due to high frequency of detection. Understudied compounds should be prioritized for toxicity studies, monitoring and potential risk evaluation in order to increase knowledge on their sources and fate for proper management.

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“…However, apart from this handicap, PCR-ELISA was found to be more cost effective as real-time PCR requires the use of costly fluorescent scoring system. If the purpose of the study does not require such high sensitivity as compared to qPCR, PCR-ELISA might just be the better option as it offers semiquantitative ability and adequate sensitivity at lower costs [9]. …”

Section: Comparisons Of Pcr-elisa With Other Pcr-based Molecular Amentioning

confidence: 99%

“…The use of PCR-ELISA as an early warning system can be extended for the detection of latent symptoms of diseases or even gene expression studies through biomarkers. Some of the studies published include the development of PCR-ELISA as a replacement method for detection and validation of gene expression studies [9] and detection of four β -thalassemia point mutations in Iranians using a PCR-ELISA genotyping system [5]. …”

Section: Applications Of Pcr-elisamentioning

confidence: 99%

Application of PCR-ELISA in Molecular Diagnosis

Sue

Yeap

Omar

et al. 2014

BioMed Research International

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Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.

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PCR–ELISA detection of estrogen receptor β mRNA expression and plasma vitellogenin induction in juvenile sole (Solea solea) exposed to waterborne 4-nonylphenol

Cited by 15 publications

References 34 publications

Development and Application of Quantitative Gene Expression Assays in Wild-Caught, Non-Model Fish Species

Development and Application of Quantitative Gene Expression Assays in Wild-Caught, Non-Model Fish Species

Preliminary Review of Sources, Fate, Analytical Challenges and Regulatory Status of Emerging Organic Contaminants in Aquatic Environments in Selected African Countries

Application of PCR-ELISA in Molecular Diagnosis

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