PCR-DGGE analysis on microbial communities in pit mud of cellars used for different periods of time

Abstract

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyse microbial community evolution in the pit mud of cellars used for different periods of time in production of Chinese Luzhou-flavour liquor. The pit mud was collected from the cellars and the microbial DNA was extracted from the microbes in the pit mud. The Bf 968 primer was used for PCR-DGGE to analyse the variable region 6 (V6) to variable region 8 (V8) of the microbial 16S rDNA. It was found that the band number, dominance, diversity and similarity of the 16S rDNA were clearly different in the DGGE patterns, because of the great diversity expressed by the different microbial communities in the different-aged cellars. It is concluded that mutual collaboration and constraint exist between the different microbial communities in the different-aged cellars, and this relationship leads to an evolutional change in the structures and in the numbers of the microbial communities in the pit mud of the cellars. Changes become more obvious with increasing age of the liquor cellars.

Analysis of Clostridium cluster I community diversity in pit mud used in manufacture of Chinese Luzhou-flavor liquor

“…In this study, pit mud samples collected from the bottom layer of cellars exhibited high H values, in agreement with previous studies where bacteria (5) and archaea (6) in pit mud were analyzed, perhaps attributable to the near neutral pH in this layer (27). Ye et al (28) also reported that the H value from DGGE patterns observed for fermented vegetable wastes was highest at pH 7.…”

“…The pit mud, specific fermented clay, contains large amounts of the functional bacteria such as Clostridium spp., Bacillus spp., and Methanobacterium spp., which are important to the fermentation process and contribute to production of aromatic compounds in Luzhou-flavor liquor (2)(3)(4). To clarify the effect of microbial mechanisms on liquor production, much research has been conducted in recent years to study the composition of pit mud microbial communities (5)(6)(7)(8)(9)(10).…”

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

“…We calculated the Shannon Wiener Diversity (H) Index of the biodiversity using the previously reported formula (Deng et al. 2012). We also attempted to perform PCR-DGGE analysis of archaea and SRB communities by using group-specific primers as described (Geets et al.…”

Comparison between Bacterial Diversity of Aged and Aging Pit Mud from Luzhou-flavor Liquor Distillery

“…For instance, Deng et al analyzed microbial community evolution in the pit mud of cellars used for different periods of time by PCR-DGGE (Deng et al, 2012;Shi et al, 2011). Although there are many advantages in using PCR-DGGE for microbial community analyses, method limitations should be recognized for correct result interpretations.…”