PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut

Müller, Jens; Schulze, Frank; Müller, Wolfgang; Hänel, Ingrid

doi:10.1016/j.vetmic.2005.10.029

Cited by 74 publications

(46 citation statements)

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“…Os genes flaA, cadF e sodB foram encontrados em 100% das linhagens de Campylobacter coli estudadas (Tabela 12), o que corrobora com dados da literatura que relatam que estes genes são essenciais no processo de colonização (Muller et al,2006;Wieczoreck;Osek, 2008;Fernandes et al,2010). A enzima codificada pelo gene sodB é uma metaloenzima que proporciona proteção contra os efeitos tóxicos dos derivados reativos de oxigênio, pois se os radicais superóxidos não são eficientemente eliminados podem vir a causar danos a componentes vitais das células bacterianas (Fridovich, 1986;Purdy et al, 1999).…”

Section: Discussionunclassified

“…No entanto, alguns fatores são reconhecidos como importantes no que se refere à virulência desse gênero e estão associados tanto a genes plasmidiais, contidos no plasmídeo pVir, quanto a genes cromossômicos. Esses fatores incluem: motilidade, quimiotaxia, capacidade de colonização, adesão as células intestinais, invasão e translocação epitelial, sobrevivência intracelular e produção de toxinas (Ketley, 1997;Muller et al, 2006;Osek, 2008;Fernandes et al, 2010).…”

Section: Genes Relacionados à Virulênciaunclassified

“…Embora as estruturas que estão normalmente envolvidas com o processo de adesão não tenham sido identificadas, muitas outras moléculas são capazes de desempenhar o papel de uma adesina, como por exemplo, as proteínas codificadas pelos genes cadF, docA, racR e dnaJ (Muller et al, 2006;Biswas et al, 2011). Estudos utilizando mutantes demonstraram que a ausência das proteínas codificadas por esses genes reduziu a capacidade de colonização dessas bactérias Ziprin et al, 1999;Muller et al, 2006).…”

Section: Genes Relacionados à Virulênciaunclassified

“…Estudos utilizando mutantes demonstraram que a ausência das proteínas codificadas por esses genes reduziu a capacidade de colonização dessas bactérias Ziprin et al, 1999;Muller et al, 2006).…”

Section: Genes Relacionados à Virulênciaunclassified

“…A pesquisa dos genes relacionados à virulência por PCR foi realizada para as 63 Os genes pesquisados foram: flaA Newell, 2000), flhA, iamA, docA (Muller et al, 2006), ciaB (Rivera-Amill et al, 2001), cdtA (Hichey et al,2000), cdtB, cdtC, virB11, racR dnaJ, pldA, (Datta et al,2003, cadF , wlaN (Wassenaar et …”

Section: Pesquisa Dos Genes Relacionados à Virulência Por Pcrunclassified

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Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Gomes¹

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Este espaço é dedicado àqueles que, de alguma forma, contribuíram para que esta dissertação fosse realizada. Não sendo viável nomeá-los a todos, há no entanto alguns a quem não posso deixar de manifestar o meu apreço e agradecimento sincero.Á Deus pela benção da vida, proteção e força para superar os obstáculos ao longo da minha jornada.À Profª Drª Juliana Pfrimer Falcão, pela orientação, pelo carinhoso apoio, partilha do saber e as valiosas contribuições para a realização desse trabalho. Acima de tudo, obrigada por continuar a acompanhar-me nesta jornada e por estimular o meu interesse pelo conhecimento. Agradeço pela confiança em mim depositada, pelo empenho, paciência e o exemplo profissional e moral durante todo esse período. Minha profunda admiração. Muito Obrigada por tudo! Á minha querida e amada Mãe pelo amor sem reservas, pelos momentos de carinho e por tornar a minha vida mais colorida. Ao meu Pai pela figura firme e segura que me empurra nos momentos mais difíceis, por tornar possível os meus sonhos e conquistas. Não tenho palavras para descrever esse Amor! Aos meus queridos irmãos Gabriela, Marcos, Carina e ao meu sobrinho João Vitor pelo incentivo, apoio e carinho. Vocês são parte de mim! Á aquele que não leva jeito com as palavras, mas tem um abraço que me acalma, um olhar que me alegra, um sorriso que conforta e um amor que me transforma. Alessandro, "Não me lembro mais qual foi nosso começo. Sei que não começamos pelo começo. Já era amor antes de ser"! strains; the flhA gene was detected in 11 (17.5%) strains; the dnaJ gene was detected in 10 (15.9%) strains; the pldA gene was detected in 7 (11.1%) strains ; the iamA gene was detected in three (4.8%) strains; the cdtC and docA genes were found in two (3.2%) strains; the cdtA and crsA were found in one (1.6%) strain and the ciaB, wlaN, virB11 and racR genes were not detected. Among the 63 strains studied, 42 were susceptible to all antimicrobials tested. Of the 21 resistant strains, 10 (15.9%) were resistante to tetracycline and doxaciclyne, six (9.5%) showed resistance to ciprofloxacin, and one (1.6%) was resistant to erythromycin. Only four (6.3%) strains were simultaneously resistant to at least two different classes of the antibiotics tested. The dendrogram of genetic similarity of Pulsed field gel electrophoresis (PFGE) grouped the 63 strains studied into two groups namely PFGE-A and PFGE-B with a genomic similarity of 44.9% among them. However, some strains isolated from humans, animals, the environment and food presented a high genotypic similarity above 80% and were subdivided into seven groups designated as PFGE-A1 to PFGE-A7. The dendrogram of genetic similarity of the SRV-flaA gene sequences grouped the strains studied into two groups namely SVR-A and SVR-B, with similarity above 83.1% among them. Besides, the deposit of the SVR sequences of the flaA gene in the online database showed that the alleles 30 and 1647 were the iv most frequently found and allowed the comparison between the strains studied with the alleles described in the d...

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Section: Discussionunclassified

Section: Genes Relacionados à Virulênciaunclassified

Section: Pesquisa Dos Genes Relacionados à Virulência Por Pcrunclassified

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Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Gomes¹

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Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources

Fındık

Ica

Onuk

et al. 2010

Trop Anim Health Prod

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The genetic diversity of 168 Campylobacter jejuni isolates originating from human (n=30), cattle (n=36), sheep (n=44), dog (n=35), and poultry (n=21) and cdt genes prevalence of the isolates were investigated. To determine the genetic diversity of these strains, random amplified polymorphic DNA-polymerase chain reaction (PCR) using a random primer (M13) was performed. The numbers of genotypes determined in human, cattle, sheep, dog, and poultry isolates were 19, 18, 17, 18, and 6, respectively. To find out the prevalence of cdt genes in C. jejuni isolates simultaneously, a multiplex PCR was performed. The prevalence of the separate cdt genes was found to vary from 69% to 100% for cdtA, 92% to 100% for cdtB, and 39% to 98% for cdtC. These rates without host discriminating were 95%, 98%, and 93% for cdtA, cdtB, and cdtC, respectively. The prevalence of all three cdt genes in strains originating from human, cattle, sheep, dog, and poultry were 87%, 67%, 84%, 89%, and 39%, respectively. These results showed the relatively high genetic heterogeneity and variation of cdt genes among C. jejuni isolates from various sources except for poultry isolates. This study gives baseline data on molecular characterization of C. jejuni strains from different sources.

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fla-typing, RAPD analysis, isolation rate and antimicrobial resistance profile of Campylobacter jejuni and Campylobacter coli of human origin collected from hospitals in Tehran, Iran

et al. 2010

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The purpose of the current study was to investigate the isolation rate, antimicrobial resistance profile and molecular typing of Campylobacter spp. recovered from patients with diarrhea in hospitals in Tehran, Iran. Over a period of 13 months, from 562 diarrheal samples 49 (8.7%) Campylobacter spp. were isolated, of which 34 (69.5%) were Campylobacter jejuni and 12 (24.5%) were identified as Campylobacter coli. Antimicrobial susceptibility and typing of the Campylobacter spp. isolates was carried out using the Kirby-Bauer disk diffusion method, fla-typing and RAPD analysis. The highest resistance in the collected Campylobacter isolates was to ofloxacin (77.5%) followed by ciprofloxacin (73.4%), nalidixic acid (69.3%), ceftazidime (53%), cefotaxime (51%) and carbenicillin (40.8%) while all the isolates were susceptible to imipenem. The results of RAPD analysis and fla-typing showed a relative high diversity and weak clonality amongst Campylobacter spp. isolates from patients with diarrhea in two hospitals in Tehran, Iran. In addition, during the current study, fla-typing proved to be more reliable and reproducible for typing of isolates. To our knowledge, this is the first study providing structural data for RAPD analysis and fla-typing of Campylobacter spp. isolates recovered from Iranian patients.

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PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut

Cited by 74 publications

References 29 publications

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources

fla-typing, RAPD analysis, isolation rate and antimicrobial resistance profile of Campylobacter jejuni and Campylobacter coli of human origin collected from hospitals in Tehran, Iran

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