PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk

Rall, Vera Lúcia Mores; Vieira, Fredson; Rall, Ricardo; Vieitis, R.L.; Júnior, Ary Fernandes; Candeias, J. A. N.; Cardoso, K. F. G.; Araújo, João Pessoa

doi:10.1016/j.vetmic.2008.05.011

Cited by 145 publications

(121 citation statements)

References 28 publications

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“…It was found, that sea gene is the most frequent gene among isolates studied by Rall et al (2008) and Ertas et al (2010). However, Normanno et al (2007) and Ote et al (2011) noticed the majority of strains with sed gene.…”

Section: Resultsmentioning

confidence: 89%

“…These results are consistent with the findings of Pelisser et al (2008) who identified 45.5% of S. aureus in 200 dairy and meat samples. Rall et al (2008) and Rosengren et al (2010) identified about 70% of strains as S. aureus in milk and fresh cheeses samples. On the other hand, in some works (Normanno et al 2007;Ertas et al 2010;Trnčíková et al 2010;Zigo et al 2011), the contamination of mostly raw milk, dairy products, but also meat and meat products by S. aureus was bellow 20%.…”

Section: Resultsmentioning

confidence: 99%

“…The oligonucleotides used in this work, their sequences, annealing temperature, number of cycles needed and size of fragments are summarised in Table 1. PCR amplification was conducted in a solution containing 5 µl HotMaster taq buffer, 1.5 µl dNTP (all deoxyribonucleotides in equimolar concentrations), 0.5 µl of HotMaster taq polymerase (5prime, Hamburg, Germany), 1 µl of each primer (Ecoli, Bratislava, Slovakia), and the DNA template -a 24 h old colony, in a final volume of 50 µl according to Pinto et al (2005), Kérouanton et al (2007), and Rall et al (2008). The negative control of amplification was performed with 5 µl of water instead of DNA template.…”

Section: Methodsmentioning

confidence: 99%

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Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Medveďová¹,

Studeničová²,

Valı́k³

et al. 2014

Czech J. Food Sci.

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Medveďová A., Studeničová A., Valík Ľ., Horváthová Z. (2014): Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products. Czech J. Food Sci., 32: 337-341.We investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Staphylococcus, and PCR detection. The PCR results revealed that 32% of S. aureus isolates possessed the selected gene for SEs enterotoxins (SEA-SEE), with sea as the most predominant gene. Neither seb nor sed genes were found throughout the collection. Additionally, the growth analysis of the isolates was performed in milk at 15°C. The growth parameters were very close to one another and were also compared with the data described in the global databases. These results provided evidence that the growth rate and lag phase duration can be determined with a high degree of reproducibility without regardless of the strain origin.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Medveďová¹,

Studeničová²,

Valı́k³

et al. 2014

Czech J. Food Sci.

View full text Add to dashboard Cite

show abstract

“…The PCR was performed with master mixture: 1 μL of template DNA, 10 μL of primer mixture (0.5 μM of each forward and reverse primer), and 25 μL of double distilled water with final volume of 50 μL (33). Thermo cycle program was adjusted asan initial denaturation for three minutes at 94°C.…”

Section: Methodsmentioning

confidence: 99%

“…Most strains of S. aureus are capable of producing enterotoxin. Staphylococcal enterotoxins are single-chain, low-molecular weight (27)(28)(29)(30)(31)(32)(33)(34) proteins. Although enterotoxins are mainly producedby some S. aureus strains, they might be produced by Staphylococcusintermedius, Staphylococcus hyicus, Staphylococcus xylosus, and Staphylococcus epidermidis.…”

Section: Introductionmentioning

confidence: 99%

Enterotoxin A Gene Barrier Staphylococcus aureus Within Traditionally Dairy Products of Tehran

Hassani¹,

Doust²,

Mobarez³

2014

Int J Enteric Pathog

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Background: Staphylococcus aureusis a serious agent that often colonize dairy products all over the world. Staphylococcal enterotoxins are the essential causes of food poisoningin human societies. Enterotoxin type A is an importantstaphylococcal exotoxin. Objectives: The aim of present study was to detect the enterotoxin producing Staphylococcus.aureus within different dairy products collected from Tehran, Iran. Materials and Methods: Two hundreds twenty dairy products samples were collected from local dealers across the city. The samples were first screened for S. aureus contaminations. All isolated strains of S. aureus were then investigated for enterotoxin a gene, usind spesific primer sets. Results: Staphylococcus aureus was isolated from 43% of dairy samples: 22% from milk and 18% from cheese samples. The SEA genes were detected in 10 isolates (22%) originated from raw milk and in two isolates (25%) from domestic cheese. Conclusions: Since, the staphylococcal enterotoxins are heat stable, heat had no effect on the toxicity of the enterotoxins within positive samples. Our primer stets confirmed previous studies that introduced PCR as rapid, sensitive, and specific method for dairy products screening system. Our data showed that routine screening and surveillance is vital for different food materials including dairy products.

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Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Forouzani‐Moghaddam,

Habibi,

Hosseini‐Safa

et al. 2024

Veterinary Medicine & Sci

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IntroductionRaw milk is a nutrient‐rich food, but it may harbour harmful bacteria, such as enterotoxigenic Staphylococcus aureus (S. aureus), which can cause staphylococcal food poisoning. Antibiotic resistance of S. aureus in raw milk can increase the risk of such infections, particularly among susceptible individuals.ObjectiveThis study aimed to investigate the prevalence of enterotoxin genes a, d, g, i and j and the antibiotic resistance of S. aureus isolated from raw milk samples.MethodsDuring a 6‐month sampling period, 60 raw milk specimens were obtained from diverse locations in Yazd province, Iran. Antibiogram profiling was conducted via the disc diffusion method. In addition, staphylococcal enterotoxin (SE) genes a, d, g, i, and j were detected through real‐time PCR analysis.ResultsBacteriological assays confirmed the presence of S. aureus in 11 samples (18.3%). All isolates demonstrated 100% resistance to penicillin G but exhibited sensitivity to vancomycin, while resistance to other antibiotics ranged from 36.4% to 45.5%. The prevalence of enterotoxin genes in these strains showed variable distribution, with sea being the predominant SE (45.5%), followed by sed (36.4%), seg (18.2), sej and sei (9.1% each).ConclusionsThis study discovered the presence of multiple enterotoxins in S. aureus strains obtained from raw milk samples. These strains also demonstrated resistance to a variety of antibiotics. Since enterotoxigenic S. aureus is known to cause human food poisoning, monitoring food hygiene practices, especially during raw milk production, is critical.

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PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk

Cited by 145 publications

References 28 publications

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Enterotoxin A Gene Barrier Staphylococcus aureus Within Traditionally Dairy Products of Tehran

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

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