PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

AL-Kaisse, Asmaa A.; Al-Thwani, Amina N.; AL-Segar, Rabab Q.

doi:10.24126/jobrc.2015.9.2.439

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…In this study the percentage of resistance for Cephems (Cephalosporin IV) including (Cefepime and Ceftazidime) Cefepime agree with [32] rate resistance of cefepime was (100%) and In this study disagree with [33] the rate was (23.5%) resistance. In this study the percentage of resistance for Ceftazidime agree with the results of [34] in Basrah, that P. aeruginosa were resistant to ceftazidime, (100%).…”

Section: Antibiotic Susceptibility Of P Aeruginosamentioning

confidence: 48%

Effect nanoparticles zirconium on bacteria growth multidrug resistance pseudomonas aerginosa isolated from burns patients

Jasim,

Hussein

2023

BIO Web Conf.

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A total of 153 samples (swabs) were taken from burned patients between the ages of 2 and 75, representing a variety of age groups and body regions. All samples were obtained from Baghdad’s government hospitals. Beginning in early September 2022 and ending in early January 2023, (48/153) isolates (31.37%) were identified as P. aeruginosa Testing for antibiotic susceptibility It was conducted using various antibiotic classes (8 antibiotics), which were assessed using the VITEK 2 compact system. The results revealed that P. aeruginosa isolates were resistant to ceftazidime and cefepime (100%) in the same rate, the resistance to Tobramycin and Ciprofloxacin was (77.08), and the maximum sensitivity to Colistin was )79.1) and resistance to imipenem, meropenem, and Levofloxacin was (52.08%), (79.18%), and (72.92%), respectively. In this study used zirconium nanoparticles for inhibition growth bacteria. The efficacy of the synthetic nanoparticles against P. aeruginosa was tested as five different concentrations (1,2.5,5,10 and 15) mg/ml were adopted, and concentrations of (5,10 and 15) mg/ml showed efficacy in inhibiting bacterial growth while (1 and 2.5) mg/ml was not given any effectiveness, the results of minimum inhibitory concentration was (5) mg\ml.

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Section: Antibiotic Susceptibility Of P Aeruginosamentioning

confidence: 48%

Effect nanoparticles zirconium on bacteria growth multidrug resistance pseudomonas aerginosa isolated from burns patients

Jasim,

Hussein

2023

BIO Web Conf.

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“…As well as, Nasih et al [2014] recorded (17%) P. aeruginosa from the patients. Another study showed that (38%) P. aeruginosa isolates among 100 burn samples in Baghdad hospitals 21 . P. aeruginosa is currently one of the major bacterial pathogens responsible for nosocomial infections and due to resistance to various antibiotics the treatment of these infections has become very diffi cult.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic detection of AmpC ?-lactamase in Pseudomonas aerogenosa isolated from Burns and Wounds in Al-Rumetha Hospitals

Abeer Mohammed Ali Al-garawyi

2020

Indian Journal of Forensic Medicine & Toxicology

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Ampicillin hydrolyzing class C β-lactamase (AmpC) producing Pseudomonas aeruginosa has been recognized to be a serious opportunistic infection and due to the wide ability to create β-lactamases made this bacteria resistance to many antibiotics.The results of the current study showed that 36(27.7%) isolates of P. aeruginosa from 130 isolates examined for cefoxitin susceptibility by disk diffusion method. Out of thirty six P. aeruginosa isolates , 29(80.6%) were cefoxitin resistant . The modified three dimension test and AmpC disck test were used confirm the production AmpC β-lactamase in all cefoxitin resistant isolates , only 23(79.3%) isolates were phenotypically confirmed to produce AmpC beta-lactamase. The result of this study showed that 12/36 ( 33.3%) isolates were positive with blaAmpC gene by conventional PCR technique .

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Distribution of blaOXA-10, blaPER-1, and blaSHV genes in ESBL-producing Pseudomonas aeruginosa strains isolated from burn patients

Polse,

Khalid,

Mero

2023

Sci Rep

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Pseudomonas aeruginosa is resistant to a wide range of extended spectrum-lactamases (ESBLs) antibiotics because it produces several kinds of ESBLs. The goal of the current investigation was to identify the bacteria that produce extended spectrum -lactamases and the genes that encode three different ESBLs, such as blaOXA-10, blaPER-1 and blaSHV genes in Pseudomonas aeruginosa isolated from burn patients. In this investigation, 71 Pseudomonas aeruginosa isolates were isolated from burn wounds in Burn and Plastic Surgery Hospital, Duhok City between July 2021 to June 2022. For the purpose of finding the blaOXA-10, blaPER-1, and blaSHV ESBL expressing genes, Polymerase Chain Reaction (PCR) was used. Among 71 Pseudomonas aeruginosa isolates, 26.36% (29/71) were isolated from males and 38.18% (42/71) from females, and 76.06% (54/71) of the isolates were multidrug resistant. They exhibited higher resistance against Piperacillin with resistance rates of 98.59%. Among the ESBL-producing isolates tested, blaOXA-10 was found in 59.26% (32), blaPER-1 was found in 44.44% (24), and blaSHV was found in 11.11% (6). All isolates must undergo antimicrobial susceptibility testing because only a few numbers of the available antibiotics are effective for the treatment of this bacterium. This will prevent the development of resistance in burn units and aids in the management of the treatment plan.

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PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

Cited by 4 publications

References 12 publications

Effect nanoparticles zirconium on bacteria growth multidrug resistance pseudomonas aerginosa isolated from burns patients

Effect nanoparticles zirconium on bacteria growth multidrug resistance pseudomonas aerginosa isolated from burns patients

Phenotypic detection of AmpC ?-lactamase in Pseudomonas aerogenosa isolated from Burns and Wounds in Al-Rumetha Hospitals

Distribution of blaOXA-10, blaPER-1, and blaSHV genes in ESBL-producing Pseudomonas aeruginosa strains isolated from burn patients

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