PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak

Dědečková, K.; Fila, Libor; Skalická, Veronika; Bartošová, Jana; Kucerova, T.; Vávrová, V.; Zemková, D; Kalferstova, Lucie; Melter, Oto; Cinek, Ondřej; Dřevı́nek, Pavel

doi:10.1016/j.jcf.2012.04.005

Cited by 16 publications

(10 citation statements)

References 20 publications

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“…Gram-negative bacteria that, while phenotypically similar, are genetically distinct species (1)(2)(3). Although Bcc strains are commonly found in the environment, Bcc bacteria are a group of opportunistic pathogens associated with immunocompromised patients, such as those with cystic fibrosis (CF) (4,5).…”

Section: T He Burkholderia Cepacia Complex (Bcc) Is Comprised Of 18mentioning

confidence: 99%

Short Palate, Lung, and Nasal Epithelial Clone 1 Has Antimicrobial and Antibiofilm Activities against the Burkholderia cepacia Complex

Ahmad

Tyrrell

Walton

et al. 2016

Antimicrob Agents Chemother

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The opportunistic bacteria of the Burkholderia cepacia complex (Bcc) are extremely pathogenic to cystic fibrosis (CF) patients, and acquisition of Bcc bacteria is associated with a significant increase in mortality. Treatment of Bcc infections is difficult because the bacteria are multidrug resistant and able to survive in biofilms. Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) is an innate defense protein that is secreted by the upper airways and pharynx. While SPLUNC1 is known to have antimicrobial functions, its effects on Bcc strains are unclear. We therefore tested the hypothesis that SPLUNC1 is able to impair Bcc growth and biofilm formation. We found that SPLUNC1 exerted bacteriostatic effects against several Bcc clinical isolates, including B. cenocepacia strain J2315 (50% inhibitory concentration [IC 50 ] ‫؍‬ 0.28 M), and reduced biofilm formation and attachment (IC 50 ‫؍‬ 0.11 M). We then determined which domains of SPLUNC1 are responsible for its antimicrobial activity. Deletions of SPLUNC1's N terminus and ␣6 helix did not affect its function. However, deletion of the ␣4 helix attenuated antimicrobial activity, while the corresponding ␣4 peptide displayed antimicrobial activity. Chronic neutrophilia is a hallmark of CF lung disease, and neutrophil elastase (NE) cleaves SPLUNC1. However, we found that the ability of SPLUNC1 to disrupt biofilm formation was significantly potentiated by NE pretreatment. While the impact of CF on SPLUNC1-Bcc interactions is not currently known, our data suggest that understanding this interaction may have important implications for CF lung disease.

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Section: T He Burkholderia Cepacia Complex (Bcc) Is Comprised Of 18mentioning

confidence: 99%

Short Palate, Lung, and Nasal Epithelial Clone 1 Has Antimicrobial and Antibiofilm Activities against the Burkholderia cepacia Complex

Ahmad

Tyrrell

Walton

et al. 2016

Antimicrob Agents Chemother

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“…B. cenocepacia was historically the most common Bcc species recovered from CF patients attending the Prague CF Centre, with an infection rate of up to 30% in the early 2000s [3]. The strain responsible for this Czech epidemic was identified by multilocus sequence typing (MLST) [4] as sequence type 32 (ST32), and this strain was also recognised as the cause of epidemic outbreaks in other countries (France, Italy, the UK and Canada, as reviewed in [5]).…”

Section: Introductionmentioning

confidence: 99%

“…The strain responsible for this Czech epidemic was identified by multilocus sequence typing (MLST) [4] as sequence type 32 (ST32), and this strain was also recognised as the cause of epidemic outbreaks in other countries (France, Italy, the UK and Canada, as reviewed in [5]). In 2001, the ST32 strain was found in nearly all Prague CF patients who suffered from Bcc infection (93%; 52 out of 56 infected patients) [3]. The spread of the infection was eventually stopped primarily as the result of a systematic infection surveillance program implemented by the CF treatment centre.…”

Section: Introductionmentioning

confidence: 99%

“…The program included regular PCR-based detection of Bcc bacteria directly from sputum isolated from each patient [6] and strain identification of clinical isolates by genotyping techniques including direct-sputum DNA based MLST [7] and random amplified polymorphic DNA (RAPD) [8]. As a consequence of these detection and subsequent clinical management measures, no new ST32-positive patients have been detected in the Prague CF community since 2004 [3].…”

Section: Introductionmentioning

confidence: 99%

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Novel diagnostic PCR assay for Burkholderia cenocepacia epidemic strain ST32 and its utility in monitoring infection in cystic fibrosis patients

Dědečková

Kalferstova

Strnad

et al. 2013

Journal of Cystic Fibrosis

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“…7 In terms of point sources, only half of the outbreak reports indicated a confirmed point source for B. cepacia HAIs. [8][9][10][11] Confirmed point sources included contaminated solutions and medications such as mouthwashes, 12 respiratory equipment and ventilators, 13 disinfectants, 14 hemodialysis machines, 15 intravenous catheters, 16 medication vials 17 and ultrasound gels. 18 The published evidence describing B. cepacia HAIs worldwide is disparate and may present challenges for infection control professionals (ICPs) attempting to source current evidence to plan intervention strategies.…”

mentioning

confidence: 99%

Global burden, point sources, and outbreak management of healthcare-associatedBurkholderia cepaciainfections: An integrative review

Shaban

Sotomayor-Castillo

Nahidi

et al. 2020

Infect. Control Hosp. Epidemiol.

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Objective:To examine the global burden, associated point sources, and successful prevention and control measures for documented outbreaks of Burkholderia cepacia healthcare-associated infections (HAIs).Design:Integrative review.Methods:A review of all outbreaks of Burkholderia cepacia HAIs published in the peer-reviewed literature between January 1970 and October 2019 was conducted to identify the global burden, associated point sources, and successful prevention and control measures using the Guidelines for Outbreak Reports and Intervention Studies of Nosocomial Infections (ORION).Results:In total, we reviewed 125 documented outbreaks of Burkholderia cepacia–related HAIs worldwide. The reported B. cepacia HAIs for this period involved 3,287 patients. The point sources were identified in most outbreaks of B. cepacia HAIs (n = 93; 74.4%); they included medication vials, disinfectants, and antiseptics. Moreover, 95 of the outbreak reports (76%) described effective prevention and control measures, but only 33 reports indicated the use of a combination of environment-, patient- and staff-related measures. None of the outbreak reports used the ORION guidelines.Conclusions:Outbreaks of Burkholderia cepacia HAIs are an ongoing challenge. They are often associated with immunocompromised patients who acquire the infection from exposure to contaminated medications, products, and equipment. These outbreaks are not infrequent, and a range of infection prevention and control measures have been effective in arresting spread. The use of ORION guidelines for outbreak reporting would improve the quality of information and data to generate evidence for translation into practice.

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PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak

Abstract: A likely factor contributing to the end of ST32 epidemic was segregation of Bcc infected patients that included also patients with no culture, but PCR positivity. The diagnostic PCR led to timely identification of cases with 'culture-invisible' infection.

Cited by 16 publications

References 20 publications

Short Palate, Lung, and Nasal Epithelial Clone 1 Has Antimicrobial and Antibiofilm Activities against the Burkholderia cepacia Complex

Short Palate, Lung, and Nasal Epithelial Clone 1 Has Antimicrobial and Antibiofilm Activities against the Burkholderia cepacia Complex

Novel diagnostic PCR assay for Burkholderia cenocepacia epidemic strain ST32 and its utility in monitoring infection in cystic fibrosis patients

Global burden, point sources, and outbreak management of healthcare-associatedBurkholderia cepaciainfections: An integrative review

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