PCR Detection Method of <i>Clostridium scindens</i> and <i>C. hiranonis</i> in Human Fecal Samples

Kitahara, Maki; Sakamoto, Mitsuo; Benno, Yoshimi

doi:10.1111/j.1348-0421.2001.tb02616.x

Cited by 23 publications

(18 citation statements)

References 16 publications

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“…, Streptococcus spp. , E. coli, Blautia spp., Fusobacterium spp., Clostridium Hiranonis )33 in the fecal microbiota were analyzed by qPCR assays, as described previously for canine fecal samples 18, 34…”

Section: Methodsmentioning

confidence: 99%

Effect of an extruded animal protein‐free diet on fecal microbiota of dogs with food‐responsive enteropathy

Bresciani

Minamoto

Suchodolski

et al. 2018

Veterinary Internal Medicne

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BackgroundDietary interventions are thought to modify gut microbial communities in healthy individuals. In dogs with chronic enteropathies, resolution of dysbiosis, along with remission of clinical signs, is expected with treatment.Hypothesis/ObjectiveTo evaluate changes in the fecal microbiota in dogs with food‐responsive chronic enteropathy (FRE) and in healthy control (HC) dogs before and after an elimination dietary trial with an animal protein‐free diet (APFD).AnimalsDogs with FRE (n = 10) and HC (n = 14).MethodsDogs were fed the APFD for 60 days. Fecal microbiota was analyzed by Illumina 16S rRNA sequencing and quantitative polymerase chain reaction (PCR).ResultsA significantly lower bacterial alpha‐diversity was observed in dogs with FRE compared with HC dogs at baseline, and compared with FRE dogs after the trial. Distinct microbial communities were observed in dogs with FRE at baseline compared with HC dogs at baseline and compared with dogs with FRE after the trial. Microbial communities still were different in FRE dogs after the trial compared with HC dogs at baseline. In HC dogs, the fecal microbiota did not show a significant modification after administration of the APFD.Conclusion and Clinical ImportanceOur results suggest that, in FRE dogs, treatment with the APFD led to a partial recovery of the fecal microbiota by significantly increasing microbiota richness, which was significantly closer to a healthy microbiota after the treatment. In contrast, no changes were detected in the fecal microbiota of HC dogs fed the same APFD.

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Section: Methodsmentioning

confidence: 99%

Effect of an extruded animal protein‐free diet on fecal microbiota of dogs with food‐responsive enteropathy

Bresciani

Minamoto

Suchodolski

et al. 2018

Veterinary Internal Medicne

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“…Early molecular studies showed that C. scindens and Eg. lenta, which both belong to the functional group of biliary steroid-metabolising bacteria, are common members of the human intestinal microbiota (Bokkenheuser et al, 1979;Doerner et al, 1997;Schwiertz et al, 2000;Kitahara et al, 2001). Besides, as in the case of SECO demethylation by P. productus, the dehydroxylation activity was observed for several strains of Eg.…”

Section: Identification Of Seco-dehydroxylating Bacteriamentioning

confidence: 99%

Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside

Clavel¹,

Henderson²,

Engst³

et al. 2006

FEMS Microbiology Ecology

172

134

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The human intestinal microbiota is essential for the conversion of the dietary lignan secoisolariciresinol diglucoside (SDG) via secoisolariciresinol (SECO) to the enterolignans enterodiol (ED) and enterolactone (EL). However, knowledge of the species that catalyse the underlying reactions is scant. Therefore, we focused our attention on the identification of intestinal bacteria involved in the conversion of SDG. Strains of Bacteroides distasonis, Bacteroides fragilis, Bacteroides ovatus and Clostridium cocleatum, as well as the newly isolated strain Clostridium sp. SDG-Mt85-3Db, deglycosylated SDG. Demethylation of SECO was catalysed by strains of Butyribacterium methylotrophicum, Eubacterium callanderi, Eubacterium limosum and Peptostreptococcus productus. Dehydroxylation of SECO was catalysed by strains of Clostridium scindens and Eggerthella lenta. Finally, the newly isolated strain ED-Mt61/PYG-s6 catalysed the dehydrogenation of ED to EL. The results indicate that the activation of SDG involves phylogenetically diverse bacteria, most of which are members of the dominant human intestinal microbiota.

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“…scindens is an indigenous bacteria in human intestine (18) and has high activity compared to other bile acid 7α-dehydroxylating bacteria (5, 16), it follows from these results that C. scindens might be the most important species in bile acid 7α-dehydroxylation.…”

Section: Quantification Of C Scindensmentioning

confidence: 67%

“…Species-specific primers for C. scindens were used as previously described (18). Realtime PCR was performed with the LightCycler TM system (Roche Molecular Biochemicals, Mannheim, Germany) and the double-stranded DNA binding dye SYBR ® Green I (Roche Molecular Biochemicals) according to Sakamoto et al (23).…”

Section: Bacterial Strain C Scindens Jcm 6567mentioning

confidence: 99%

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Comparison among Fecal Secondary Bile Acid Levels, Fecal Microbiota and Clostridium scindens Cell Numbers in Japanese

Kitahara

Sakata

Sakamoto

et al. 2004

Microbiology and Immunology

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Bile acid 7α‐dehydroxylation by intestinal bacteria, which converts cholic acid and chenodeoxycholic acid to deoxycholic acid (DCA) and lithocholic acid (LCA), respectively, is an important function in the human intestine. Clostridium scindens is one of the most important bacterial species for bile acid 7α‐dehydroxylation because C. scindens has high levels of bile acid 7α‐dehydroxylating activity. We quantified C. scindens and secondary bile acids, DCA and LCA, in fecal samples from 40 healthy Japanese and investigated their correlation. Moreover, we used terminal restriction fragment length polymorphism (T‐RFLP) analysis to investigate the effect of fecal microbiota on secondary bile acid levels. There was no correlation between C. scindens and secondary bile acid in fecal samples. On the other hand, T‐RFLP analysis demonstrated that fecal microbiota associated with high levels of DCA were different from those associated with low levels of DCA, and furthermore that fecal microbiota in the elderly (over 72 years) were significantly different from those in younger adults (under 55 years). These results suggest that intestinal microbiota have a stronger effect on DCA level than does the number of C. scindens cells.

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PCR Detection Method of Clostridium scindens and C. hiranonis in Human Fecal Samples

Cited by 23 publications

References 16 publications

Effect of an extruded animal protein‐free diet on fecal microbiota of dogs with food‐responsive enteropathy

Effect of an extruded animal protein‐free diet on fecal microbiota of dogs with food‐responsive enteropathy

Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside

Comparison among Fecal Secondary Bile Acid Levels, Fecal Microbiota and Clostridium scindens Cell Numbers in Japanese

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