PCR detection and PFGE genotype analyses of streptococcal clinical isolates from tilapia in China

Development of Streptococcus agalactiae vaccines for tilapia

“…com/ articles/ 812/ streptococcosis-in-tilapiaa-more-complex-problem/). In China, more than 90% of the clinical bacterial isolates from infected tilapia since 2009 have been S. agalactiae (Chen et al 2012a). To date, S. agalactiae infection in tilapia outbreaks have been reported in several countries, including the USA (Evans et al 2006), China (Liu et al 2012), Kuwait (Evans et al 2002), Israel (Eldar et al 1994), Thailand (Suanyuk et al 2008), Honduras (Delannoy et al 2012), and Brazil (Mian et al 2009), resulting in serious annual economic losses.…”

Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China

“…China, the largest tilapia producer in the world (Amal and Zamri-Saad, 2011), has suffered from a continuously severe and extensive outbreak of streptococcosis in cultured tilapia for years, particularly in the south provinces including Guangdong, Guangxi, Hainan, and Fujian, for years, causing very serious economic losses yearly since 2009. The cumulative mortality were 30-80% (Chen et al, 2012a(Chen et al, , 2012b, and some ponds suffered from total crop failure because of this disease. Furthermore no chemotherapeutic or immunological measures have been developed to prevent or control this disease effectively so far.…”

“…Furthermore no chemotherapeutic or immunological measures have been developed to prevent or control this disease effectively so far. The causative agent has been identified in many studies as S. agalactiae (Chen et al, 2012b;Suanyuk et al, 2008;Ye et al, 2011). However, despite the significant impact of S. agalactiae as an infectious agent, few reports on the genetic characterization of clinical tilapia GBS isolates circulating in China exist, and few epidemiological studies on the effects of this bacterium on infected fish have been published.…”

“…The characterization of S. agalactiae strains from human and mammal sources has been studied using a broad range of methods, such as molecular serotyping, random amplified polymorphic DNA, amplified fragment length polymorphism (AFLP), enterobacterial repetitive intergenic consensus, pulsed field gel electrophoresis, multilocus sequence typing (MLST), and multiple-locus variant-repeat assay (MLVA) (Chen et al, 2012b;Delannoy et al, 2013;Evans et al, 2008;Hernández et al, 2009;Imperi et al, 2010;Olivares-Fuster et al, 2008;Pereira et al, 2010;Suanyuk et al, 2008;Ye et al, 2011). Recently, phage typing was used in epidemiological investigations because lysogeny was specific to each intraspecific lineage of GBS strain (Salloum et al, 2011).…”

Complete Genome Sequence of Streptococcus agalactiae GD201008-001, Isolated in China from Tilapia with Meningoencephalitis

“…However, most studies have been focused on S. agalactiae isolated from humans and cows. Since 2009, an outbreak of serious GBS infectious disease in tilapia cultural farms in the south of China has occurred, causing high mortalities and large economic losses (1). S. agalactiae strain GD201008-001 was isolated from moribund cultural tilapia with meningoencephalitis in the Guangdong province of China in 2010 and belongs to serotype Ia, multilocus sequence type 7 (ST7).…”