PCR-detected hepatitis C virus RNA associated with human zona-intact oocytes collected from infected women for ART

Papaxanthos-Roche, Aline; Trimoulet, Pascale; Commenges-Ducos, M.; Hocké, C.; Fleury, Hervé; Mayer, Gal

doi:10.1093/humrep/deh220

Cited by 12 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hepatitis C virus (HCV) has been found in the FF of infected women (45) and was found to be associated with zona-intact oocytes from hepatitis C virus RNA-positive women undergoing IVF (including oocytes fertilized through ICSI) (46). But no cross-contamination of either of these viruses in embryo cryostorage has been reported.…”

Section: Evidence Of Risksmentioning

confidence: 99%

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Pomeroy

Harris

Conaghan

et al. 2010

Fertility and Sterility

View full text Add to dashboard Cite

Section: Evidence Of Risksmentioning

confidence: 99%

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Pomeroy

Harris

Conaghan

et al. 2010

Fertility and Sterility

View full text Add to dashboard Cite

“…Therefore, the authors concluded that follicular fluid must be considered as potentially infected, but all oocytes can be inseminated by ICSI resulting in virus-free embryos in culture. In a smaller study, Papaxanthos-Roche et al (2004) found HCV associated with unfertilized oocytes denuded of follicular cells.…”

Section: Virus-positive Femalementioning

confidence: 90%

Guidelines for risk reduction when handling gametes from infectious patients seeking assisted reproductive technologies

Jindal

Rawlins

Müller

et al. 2016

Reproductive BioMedicine Online

View full text Add to dashboard Cite

According to the Americans with Disabilities Act (1990), couples with blood-borne viruses that lead to infectious disease cannot be denied fertility treatment as long as the direct threat to the health and safety of others can be reduced or eliminated by a modification of policies or procedures. Three types of infectious patients are commonly discussed in the context of fertility treatment: those with human immunodeficiency virus (HIV), hepatitis C or hepatitis B. Seventy-five per cent of hepatitis C or HIV positive men and women are in their reproductive years, and these couples look to assisted reproductive techniques for risk reduction in conceiving a pregnancy. In many cases, only one partner is infected. Legal and ethical questions about treatment of infectious patients aside, the question most asked by clinical embryologists and andrologists is: "What are the laboratory protocols for working with gametes and embryos from patients with infectious disease?" The serostatus of each patient is the key that informs appropriate treatments. This guidance document describes protocols for handling gametes from seroconcordant and serodiscordant couples with infectious disease. With minor modifications, infectious patients with stable disease status and undetectable or low viral load can be accommodated in the IVF laboratory.

show abstract

“…The aim of Papaxanthos-Roche's study [14] was to investigate the susceptibility of human oocytes from hepatitis C virus (HCV) RNA-positive women to HCV contamination during assisted reproductive technology (ART). A reverse transcriptase PCR assay was used to test for the presence of HCV RNA associated with 24 unfertilized oocytes 48 h after follicular fluid aspiration in 10 IVF attempts (seven conventional IVF and three ICSI).…”

Section: Discussionmentioning

confidence: 99%

“…A weak correlation was found between plasma and follicular fluid HCV RNA loads (r = 0.73, P \ 0.001). HCV associated with unfertilized oocytes surrounded by their intact zona pellucida from anti-HCV antibody-positive and viraemic women undergoing ART raises questions concerning the safe management of medically assisted procreation for these women and good practice of oocyte/ embryo cryopreservation and donation [14].…”

Section: Discussionmentioning

confidence: 99%

Viral Hepatitis and Assisted Reproduction

Allahbadia

2015

J Obstet Gynecol India

View full text Add to dashboard Cite

Infertility treatment in couples where one or both parents are infected with hepatitis raises many concerns about transmission of the infection to the baby, laboratory technicians, and medical staff, and contamination of other gametes/embryos that are from virus-free parents in the same laboratory. Exposure to the other partner is only a risk when the couple's hepatitis status is discordant. The management of infertility, in association with HBV or HCV, has sparked debates about the potential risk of spread of infection to virus-free individuals, embryos, and/or semen. This risk can only be minimized or eliminated by the use of SOPs for safety in fertility clinics and by the use of proper initial detection and segregation of potentially hazardous materials. HBV may interfere with the development of embryos and even result in abortion and other adverse outcomes. Although sexual transmission of HCV is very low, in subfertile or infertile couples sperm washing should be used to treat HCV-positive semen before ART.Testing for HBsAg and HCV should be offered to high-risk infertile couples seeking fertility therapy to reduce the potential risk of transmission to an uninfected partner, baby, staff members, and disease-free gametes and embryos in the same laboratory. Testing for HIV, HBsAg,

show abstract

PCR-detected hepatitis C virus RNA associated with human zona-intact oocytes collected from infected women for ART

Cited by 12 publications

References 23 publications

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Guidelines for risk reduction when handling gametes from infectious patients seeking assisted reproductive technologies

Viral Hepatitis and Assisted Reproduction

Contact Info

Product

Resources

About