PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize

Jurado, Miguel; Vázquez, Covadonga; Marı́n, Sonia; Sanchis, Vicente; González-Jaén, M. Teresa

doi:10.1016/j.syapm.2006.01.014

Cited by 119 publications

(97 citation statements)

References 19 publications

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“…In any event, the low level of non-toxigenic strains (less than 20%), the importance of some of the toxins produced, and their wide occurrence in cereals, highlight the potential contribution of F equiseti to the toxin risk associated with the consumption of Spanish cereals, as well as the need to design early detection and control strategies for this species. The PCR-based F equiseti detection protocol (Jurado et al, , 2006b) may be useful in this respect.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, these techniques have provided the basis necessary for developing rapid, specific and accurate diagnostic methods based on PCR. These can be used to predict mycotoxin risk, providing the information necessary for early control strategies to be adopted (Jurado et al, , 2006bKnutsen et al, 2004;Konstantinova and Yli-Mattila, 2004). Several genomic sequences have been used to analyse intraspecific variability in Fusarium, including intron regions of histone coding genes, the P-tubulin gene ((STUB), the calmodulin gene (O'Donnell et al, 1998a;Steenkamp et al, 2002), and the translation elongation factor gene EF-\a (O'Donnell et al, 1998b(O'Donnell et al, , 2000.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

et al. 2012

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Fusarium equiseti and Fusarium acuminatum are toxigenic species that contaminate cereal crops from diverse climatic regions. They are common in Spanish cereals. The information available on their phylogenetics and toxigenic profiles is, however, insufficient to assist risk evaluation. In this work, phylogenetic analyses were performed using partial sequences of the translation elongation factor gene (EF-\a) of F. equiseti and F. acuminatum strains isolated from barley and wheat from Spain and other countries. The Northern and Southern European F. equiseti strains largely separated into two phylogenetically distinct clusters. This suggests the existence of two distinct populations within this species, explaining its presence in these regions of markedly different climate. Production of type A and B trichothecenes by the Spanish strains, examined in wheat cultures using a multitoxin analytical method, indicated that F. equiseti could produce deoxynivalenol and nivalenol and other trichothecenes, at concentrations that might represent a significant risk of toxin contamination for Southern European cereals. F. acuminatum showed low intraspecific genetic variability and 58% of the strains could produce deoxynivalenol at low level. Neither species was found to produce T-2 or HT-2 toxins. The present results provide important phylogenetic and toxigenic information essential for the accurate prediction of toxigenic risk.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

et al. 2012

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“…DNA extraction was then carried out as described previously (Querol et al, 1992). A previously described speciesspecific PCR protocol (Jurado et al, 2006) was used to confirm the identification of the isolates using the primer set Fp3-F/Fp4-R (5'-CGGCCACCAGAGGATGTG-375'-AACACGAATCGCTTCCTGAC-3').…”

Section: Morphological and Molecular Identification Of Isolatesmentioning

confidence: 99%

The effects of storage duration, temperature and cultivar on the severity of garlic clove rot caused by Fusarium proliferatum

Llamas

Patón

Díaz³

et al. 2013

Postharvest Biology and Technology

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Diseases that affect garlic during storage can lead to severe economic losses for farmers worldwide. One causal agent of clove rot is Fusarium proliferatum. Here, the progress of clove rot caused by F. proliferatum and its dependence on different storage conditions and cultivar type were studied. The effect of temperature on mycelial growth, conidial viability, and fungal survival during garlic commercial storage was documented. Samples of 50 bulbs from a randomized field trial with three different clonal generations for purple garlic (F3, F4 and F5) and the F4 clonal generation for white garlic were labeled and stored for two months (short-term storage). In addition, another sample of the F5 clonal generation of purple garlic was stored for 6 months after harvest (long-term storage). The presence of the pathogen and the percentage of symptomatic cloves were evaluated. A notable difference in the rot severity index (RSI) of different garlic varieties was observed. In all studied cases, clove rot increased with storage time at 20 °C, and the white garlic variety had a higher index of rot severity after two months of storage. Additionally, there were clear differences between the growth rates of F. proliferatum isolates.Studies conducted on the temperature responses of the pathogen propagules showed that exposure for at least 20min at 50 °C was highly effective in significantly reducing the viability of fungal conidia.Pathogenicity studies showed that the fungus is pathogenic in all commercial varieties. However, there were significant differences in varietal susceptibility between Chinese and white garlic type cultivars (81.84 ± 16.44% and 87.5 ± 23.19% symptomatic cloves, respectively) and purple cultivars (49.06 ± 13.42% symptomatic cloves).

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“…PCR amplification of a portion of thel IGS region was carried out with primers Gib2-F (5' GAGGCGCGGT GTCGGTGTGCTTG 3') and Fgc-R (5' CTCTCATA TACCCTCCG 3') (Jurado et al 2006). The amplification protocol was 1 cycle of 85 s at 94°C, 25 cycles of 35 s at 95°C (denaturation), 30 s at 58°C (annealing), 30 s at 72°C (extension), and 1 cycle of 10 min at 72°C This PCR assay amplifies an internal fragment within the IGS region of ~1000 bp (approximately from nucleotides 780 to 1780) in all of the species of the Gibberella fujikuroi complex tested.…”

Section: Fungal Isolatesmentioning

confidence: 99%

Divergence of the IGS rDNA in Fusarium proliferatum and Fusarium globosum reveals two strain specific non-orthologous types

et al. 2010

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A phylogenic analysis of Fusarium proliferatum and closely related species was performed using the most variable part within the intergenic spacer of the nuclear ribosomal DNA (IGS) and compared with a previously reported phylogeny performed in the same group of samples with a partial region of the nuclear single copy gene encoding the elongation factor la (EF-la). The phytogenies from both genomic sequences were not concordant and revealed the presence of two nonorthologous IGS types, named types I and II, in F. proliferatum and Fusarium globosum.Two specific PCR assays designed to amplify either IGS type I or type II revealed that only one IGS type was present in each individual in these two species. The presence of both IGS types at the species level indicates that homogenization has not been achieved yet. This might be retarded if panmictic sexual reproduction was affected by certain levels of clonal reproduction and/or by the diverse hosts that these species are able to colonize. This study indicates that taxonomic studies carried out with the IGS rDNA, which has been widely used in Fusarium, should be undertaken with caution.

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PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize

Cited by 119 publications

References 19 publications

Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

The effects of storage duration, temperature and cultivar on the severity of garlic clove rot caused by Fusarium proliferatum

Divergence of the IGS rDNA in Fusarium proliferatum and Fusarium globosum reveals two strain specific non-orthologous types

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