PCR-based molecular diagnosis of botulism (types C and D) outbreaks in aquatic birds

Chellapandi, P.; Prisilla, A.

doi:10.1007/s13213-018-1390-7

Cited by 5 publications

(1 citation statement)

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“…Approximately 200 closely related species are found within the Clostridium genus, which limits to differentiate them from each other and for accurate identification of individual strains (Chellapandi and Prisilla, 2018;Lindstrom and Korkeala, 2006). We observed phylogenetic proximity between strain PCP07 and C. botulinum type C isolated from the geographic regions of Georgia and Sweden.…”

Section: Resultsmentioning

confidence: 83%

A human–food web–animal interface on the prevalence of food-borne pathogens (Clostridia and Enterococcus) in mixed veterinary farms

Prisilla

Remin

Roja

et al. 2019

Food Sci Biotechnol

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In the present work, we addressed the impact of a human-food web-animal interface on the prevalence of food-borne pathogens in mixed farms of Tamil Nadu, India. We have isolated and identified six strains of Clostridium sp. and five strains of Enterococcus sp. from food and animal sources disposed near to the veterinary and poultry farms. Phylogenetic relationships of these strains were inferred from their homologies in 16S rDNA sequences and rRNA secondary structures. The strain PCP07 was taxonomically equivalent to C. botulinum confirmed by neurotoxin-specific PCR primers, followed by mouse bioassay. Other Clostridial and Enterococcal isolates have shown a phylogenetic similarity to the C. bifermentans and E. durans isolated from veterinary farms, respectively. Results of our study revealed that a humanfood web-animal interface has influenced the disease incidence and prevalence of these isolates in the poultry to veterinary farms, where human food acted as a likely transmittance vehicle for their infections.

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Section: Resultsmentioning

confidence: 83%