PCR-based microsatellite analysis for differentiation and genetic                monitoring of nine inbred SENCAR mouse strains

Benavides, Fernando; Glasscock, Edward; Coghlan, Lezlee G; Stern, Mariana C.; Weiss, Deborah A.; Conti, Claudio J.

doi:10.1258/0023677011911534

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some strains are long-lived with little spontaneous early pathology, while others develop diseases such as cancer, diabetes, obesity, and atherosclerosis and can be used as models of these conditions. Some strains were selected before they were fully inbred for characters like hypertension (de Jong 1984), “emotionality” (Broadhurst 1975), learning ability (Amit and Smith 1992), and sensitivity to carcinogens (Benavides et al 2000; Fischer et al 1987; Stern et al 1998). A small collection of inbred strains of rats or mice will usually differ for nearly all biological characteristics as a result of the chance fixation of the genes present in the stock from which they were derived.…”

Section: Definition and Properties Of Inbred Strainsmentioning

confidence: 99%

Inbred Strains Should Replace Outbred Stocks in Toxicology, Safety Testing, and Drug Development

Festing¹

2010

Toxicol Pathol

113

View full text Add to dashboard Cite

Methods of toxicity testing, barely changed for several decades, need to be improved. One way forward would be to use a small battery of inbred strains instead of the single outbred stock currently used in toxicity screening. Inbred strains are more stable, more uniform, more repeatable, and better defined than outbred stocks. Genetic variation would be observed as the difference between strains. Safety could be based on the most susceptible strain. Sometimes it may be possible to identify the genes involved. Mechanisms could be explored using gene expression profiling of susceptible and resistant strains. Two committees of toxicologists have concluded that the use of inbred strains, by controlling interindividual variability, would reduce the number of animals needed in toxicity screening, although both ''preferred'' outbred stocks. This preference appears to have been based on intuition rather than scientific principles. Data from a previously published study on the response to chloramphenicol in an outbred stock and four inbred strains is used to explain the advantages of the multistrain design. Toxicologists, safety pharmacologists, regulatory authorities, and pharmaceutical companies should take a critical look at the types of animals they use if they want to reduce the attrition rate of new drugs.

show abstract

Section: Definition and Properties Of Inbred Strainsmentioning

confidence: 99%

Inbred Strains Should Replace Outbred Stocks in Toxicology, Safety Testing, and Drug Development

Festing¹

2010

Toxicol Pathol

113

View full text Add to dashboard Cite

show abstract

“…The PCR products were resolved by 2% agarose gel and ethidium bromide staining. The genotyping for the microsatellite markers linked to Idd loci (Idd1-Idd15) was performed using genomic DNA from the fourth generation of mice from the aforementioned NOD and NOR backcrosses (20,21), with a set of 11 primers specific for polymorphic loci between NOD/CD-1 and NOR/CD-1: D6Mit52, D3Nds36, D2Mit107, D4Mit202, D1Mit24, D5Mit69, D11Mit320, D7Mit20, D7Nds6, D14Nds3, and D3Nds6. The chosen markers were evenly spaced along the genome.…”

Section: Genotypingmentioning

confidence: 99%

IFNβ Accelerates Autoimmune Type 1 Diabetes in Nonobese Diabetic Mice and Breaks the Tolerance to β Cells in Nondiabetes-Prone Mice

Alba

Puertas

Carrillo

et al. 2004

The Journal of Immunology

View full text Add to dashboard Cite

Genetic and environmental factors are decisive in the etiology of type 1 diabetes. Viruses have been proposed as a triggering environmental event and some evidences have been reported: type I IFNs exist in the pancreata of diabetic patients and transgenic mice expressing these cytokines in β cells develop diabetes. To determine the role of IFNβ in diabetes, we studied transgenic mice expressing human IFNβ in the β cells. Autoimmune features were found: MHC class I islet hyperexpression, T and B cells infiltrating the islets and transfer of the disease by lymphocytes. Moreover, the expression of β2-microglobulin, preproinsulin, and glucagon in the thymus was not altered by IFNβ, thus suggesting that the disease is caused by a local effect of IFNβ, strong enough to break the peripheral tolerance to β cells. This is the first report of the generation of NOD (a model of spontaneous autoimmune diabetes) and nonobese-resistant (its homologous resistant) transgenic mice expressing a type I IFN in the islets: transgenic NOD and nonobese-resistant mice developed accelerated autoimmune diabetes with a high incidence of the disease. These results indicate that the antiviral cytokine IFNβ breaks peripheral tolerance to β cells, influences the insulitis progression and contributes to autoimmunity in diabetes and nondiabetes- prone mice.

show abstract

“…Microsatellite markers, also known as Simple Sequence Length Polymorphisms (SSLPs) or Short Tandem Repeats (STRs), are still used in modern GeMo programmes because they are inexpensive and easy to type. 38,39 Animals are genotyped by analysing PCR-products amplified from short, tandemly arranged, repeating DNA sequences. These repeats are typically 2–6 bp long and are repeated a few to dozens of times creating allelic diversity among stains.…”

Section: Genetic Quality Control Programmesmentioning

confidence: 99%

Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report

et al. 2019

View full text Add to dashboard Cite

Genetic quality assurance (QA), including genetic monitoring (GeMo) of inbred strains and background characterization (BC) of genetically altered (GA) animal models, should be an essential component of any QA programme in laboratory animal facilities. Genetic quality control is as important for ensuring the validity of the animal model as health and microbiology monitoring are. It should be required that studies using laboratory rodents, mainly mice and rats, utilize genetically defined animals. This paper, presented by the FELASA Working Group on Genetic Quality Assurance and Genetic Monitoring of Laboratory Murines, describes the objectives of and available methods for genetic QA programmes in rodent facilities. The main goals of any genetic QA programme are: (a) to verify the authenticity and uniformity of inbred stains and substrains, thus ensuring a genetically reliable colony maintenance; (b) to detect possible genetic contamination; and (c) to precisely describe the genetic composition of GA lines. While this publication focuses mainly on mouse and rat genetic QA, the principles will apply to other rodent species some of which are briefly mentioned within the context of inbred and outbred stocks.

show abstract

PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

Cited by 8 publications

References 7 publications

Inbred Strains Should Replace Outbred Stocks in Toxicology, Safety Testing, and Drug Development

Inbred Strains Should Replace Outbred Stocks in Toxicology, Safety Testing, and Drug Development

IFNβ Accelerates Autoimmune Type 1 Diabetes in Nonobese Diabetic Mice and Breaks the Tolerance to β Cells in Nondiabetes-Prone Mice

Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report

Contact Info

Product

Resources

About