PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA

Hong, Xiao-Kun; Zhang, Xuan; Fusco, Octavio Alejandro; Lan, You-Gen; Lun, Zhao‐Rong; Lai, De‐Hua

doi:10.1016/j.actatropica.2017.04.007

Cited by 12 publications

(13 citation statements)

References 27 publications

(35 reference statements)

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“…musculi [ 95 ]. Moreover, these two species appear also difficult to distinguish on a molecular point of view [ 95 , 96 ]. We first tested the molecular methods used in our study for T .…”

Section: Discussionmentioning

confidence: 99%

“…lewisi from T . musculi [ 96 ] was also tested. The results obtained in our assays were non reproducible, non-specific and not sensitive enough ( S4C and S4D Fig ).…”

Section: Discussionmentioning

confidence: 99%

“…musculi samples were extracted independently from the same blood sample of an infected mouse. Primers used were TM1F, TM1R [ 96 ]. In the lower part, amplification of the same samples with the 18S rRNA PCR showed that the T .…”

Section: Supporting Informationmentioning

confidence: 99%

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Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal

et al. 2018

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Bioinvasion is a major public health issue because it can lead to the introduction of pathogens in new areas and favours the emergence of zoonotic diseases. Rodents are prominent invasive species, and act as reservoirs in many zoonotic infectious diseases. The aim of this study was to determine the link between the distribution and spread of two parasite taxa (Leishmania spp. and Trypanosoma lewisi) and the progressive invasion of Senegal by two commensal rodent species (the house mouse Mus musculus domesticus and the black rat Rattus rattus). M. m. domesticus and R. rattus have invaded the northern part and the central/southern part of the country, respectively. Native and invasive rodents were caught in villages and cities along the invasion gradients of both invaders, from coastal localities towards the interior of the land. Molecular diagnosis of the two trypanosomatid infections was performed using spleen specimens. In the north, neither M. m. domesticus nor the native species were carriers of these parasites. Conversely, in the south, 17.5% of R. rattus were infected by L. major and 27.8% by T. lewisi, while very few commensal native rodents were carriers. Prevalence pattern along invasion gradients, together with the knowledge on the geographical distribution of the parasites, suggested that the presence of the two parasites in R. rattus in Senegal is of different origins. Indeed, the invader R. rattus could have been locally infected by the native parasite L. major. Conversely, it could have introduced the exotic parasite T. lewisi in Senegal, the latter appearing to be poorly transmitted to native rodents. Altogether, these data show that R. rattus is a carrier of both parasites and could be responsible for the emergence of new foci of cutaneous leishmaniasis, or for the transmission of atypical human trypanosomiasis in Senegal.

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“…musculi [ 95 ]. Moreover, these two species appear also difficult to distinguish on a molecular point of view [ 95 , 96 ]. We first tested the molecular methods used in our study for T .…”

Section: Discussionmentioning

confidence: 99%

“…lewisi from T . musculi [ 96 ] was also tested. The results obtained in our assays were non reproducible, non-specific and not sensitive enough ( S4C and S4D Fig ).…”

Section: Discussionmentioning

confidence: 99%

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Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal

et al. 2018

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“…Due to these desirable attributes which are shared with mtDNA, phylogenies based on the complete maxicircle genome should inexplicably improve the robustness of the phylogenetic trees generated, making them ideal markers for phylogenetic inference. The superiority of maxicirclebased phylogenies was demonstrated in the analyses of trypanosomes, providing novel insights into the biological features of this genus (Botero et al, 2018;Hong et al, 2017;Lin et al, 2015;Messenger et al, 2012;Simpson and Simpson, 1980). It is proposed that the use of the entire coding region of the maxicircle genome will provide a much-needed framework for the taxonomic classification of the Trypanosomatidae, specifically the Leishmania genus which is studied here.…”

Section: Introductionmentioning

confidence: 99%

The complete coding region of the maxicircle as a superior phylogenetic marker for exploring evolutionary relationships between members of the Leishmaniinae

Kaufer

Barratt

Stark

et al. 2019

Infection, Genetics and Evolution

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The mitochondrial DNA (mtDNA) is a potentially valuable phylogenetic marker given its presence across all eukaryotic taxa and its relative conservation in structure and sequence. In trypanosomatids, a homologue of the mtDNA referred to as the maxicircle DNA, is located within a specialised structure in the single mitochondrion of the trypanosomatids called the kinetoplast; a high molecular weight network of DNA composed of thousands of catenated minicircles and a smaller number of larger maxicircles. Unique to the kinetoplastid protists, the maxicircle component of this complex network could represent a desirable target for taxonomic inquiry that may also facilitate exploration of the evolutionary history of this important group of parasites. The aim of this study was to investigate the phylogenetic value of the trypanosomatid maxicircle for these applications. Maxicircle sequences were obtained either by assembling raw sequence data publicly accessible in online databases (i.e., NCBI), or by amplification of novel maxicircle sequences from trypanosomatid DNA using long-range (LR) PCR with subsequent Illumina sequencing. This procedure facilitated the generation of nearly complete maxicircle sequences (i.e., excluding the divergent region) for numerous dixenous and monoxenous trypanosomatid species. Annotation of each maxicircle sequence confirmed that their structure was conserved across all taxa examined. Phylogenetic analyses confirmed that Z. australiensis showed a greater genetic relatedness with the dixenous trypanosomatids of the genera Leishmania and Endotrypanum, as opposed to members of the monoxenous genera Crithidia and Leptomonas. Additionally, molecular clock analysis supported that the dixenous Leishmaniinae appeared approximately 75 million years ago during the breakup of Gondwana. In line with previous studies, our results support the Supercontinents hypothesis regarding the origin of dixenous Leishmaniinae. Ultimately, we demonstrate that the maxicircle represents an excellent phylogenetic marker for studying the evolutionary history of trypanosomatids, resulting in trees with very high bootstrap support values.

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“…Os roedores têm importante papel na transmissão de zoonoses, como reservatórios de parasitas, entre eles: Leishmania spp, T. cruzi, T. evansi, T. rangeli e T. conorhini (DEANE; DEANE, 1961;PINTO et al, 2006;NAIFF;BARRETT, 2013;HONG et al, 2017).…”

Section: Pequenos Mamíferos Terrestresunclassified

>b<Descrição morfológica e Filogenia de parasitas do gênero >i<Trypanosoma>/i< em pequenos mamíferos silvestres do Brasil>/b<

Ferreira¹

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Tenho a impressão de ter sido uma criança brincando à beira-mar, divertindome em descobrir uma pedrinha mais lisa ou uma concha mais bonita que as outras, enquanto o imenso oceano da verdade continua misterioso diante de meus olhos."Isaac Newton RESUMO FERREIRA, J. I. G. S. Descrição morfológica e Filogenia de parasitas do gênero Trypanosoma em pequenos mamíferos silvestres do Brasil. 64 f. Tese

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PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA

Cited by 12 publications

References 27 publications

Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal

Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal

The complete coding region of the maxicircle as a superior phylogenetic marker for exploring evolutionary relationships between members of the Leishmaniinae

>b<Descrição morfológica e Filogenia de parasitas do gênero >i<Trypanosoma>/i< em pequenos mamíferos silvestres do Brasil>/b<

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