PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth

Çoğulu, Dilşah; Uzel, Ataç; Önçağ, Özant; Eronat, Cemal

doi:10.1016/j.tripleo.2008.03.004

Cited by 61 publications

(99 citation statements)

References 40 publications

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“…4,5 In addition, there are no reports in the literature that compare the microbial composition of PCs with that of RCs of infected primary teeth. Many anaerobic microorganisms are difficult to culture and identify precisely; thus, molecular methods have been used to identify microorganisms from endodontic infections.…”

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confidence: 99%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

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An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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“…28 Among different bacteria existing in infected pulp tissue, E. faecalis (facultative anaerobic gram positive cocci) is a common endodontic pathogen in teeth with failure in pulpectomy. 7,[28][29][30] The results of the study by Cogulu et al show that E. faecalis is strongly related to periapical radiolucency and pain in primary and permanent teeth. 7 Therefore, elimination of bacteria such as E. faecalis from the root canal is considered as a challenge in pulpectomy of primary and permanent teeth.…”

Section: Discussionmentioning

confidence: 99%

“…7,[28][29][30] The results of the study by Cogulu et al show that E. faecalis is strongly related to periapical radiolucency and pain in primary and permanent teeth. 7 Therefore, elimination of bacteria such as E. faecalis from the root canal is considered as a challenge in pulpectomy of primary and permanent teeth. 31 In order to solve this problem, laser has been introduced recently for disinfecting root canals.…”

Section: Discussionmentioning

confidence: 99%

“…It should be considered that the proper amount of energy for elimination of different species of bacteria may be different, for example according to the results of the study by Noiri et al on the effects of Er:YAG laser, the amount of energy that is adequate for complete elimination of gram negative species, can only decrease the growth of gram positive species. 26 Therefore, despite the fact that 100 mJ energy is not adequate for complete elimination of E. faecalis from the root canal, it is possible that regarding the multi-bacterium nature of pulp disease, 7,55 this laser with the investigated parameters is suitable for disinfection of root canal from other bacteria. In addition to mechanical methods and new tools such as laser, chemical materials such as NaOCl are used for disinfecting the canal.…”

Section: Anti-bacterial Effect Of Er:yag Laser On Enterococcus Faecalismentioning

confidence: 99%

“…[4][5][6] Moreover, the results of the study by Cogulu et al indicate strong relationship of E. faecalis bacterium with periapical radiolucency and pain in primary teeth. 7 The existence of this bacterium in root canal increases the probability of failure in root canal therapy and also in retreatment. 8,9 According to another study E. faecalis may remain in the canal after mechanical and chemical preparation of the canal.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

et al. 2017

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Introduction: Successful root canal therapy depends on the complete elimination of microorganisms such as Entroccocus faecalis, which is impossible to achieve with the traditional methods. Lasers are recently introduced as a new method to solve the problem. The present study is planned and performed to examining the antibacterial effect of Er: YAG laser. Methods: Sixty extracted anterior primary teeth were prepared and sterilized. E. faecalis bacterium was cultured in canals. Samples were randomly divided into two groups. The first group was disinfected by NaOCl 5/25% and Er: YAG laser and the second group just by NaOCl 5/25%. Samples of canal contents were cultured and colony counts were calculated. The results were analyzed statistically by SPSS software and Mann Whitney test. Results: There was no significant difference between colony counts in both groups (P = 0.142). But the number of colonies in the first group was lower than in the second group. Conclusion: Although, Er: YAG laser cannot completely eliminate E. faecalis bacterium, its simultaneous use with NaOCl decreases E. faecalis.

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Fungi in Endodontic Infections

Şen¹,

Baksı²

2017

Endodontic Microbiology

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PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth

Cited by 61 publications

References 40 publications

An investigation of the presence of specific anaerobic species in necrotic primary teeth

An investigation of the presence of specific anaerobic species in necrotic primary teeth

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

Fungi in Endodontic Infections

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