PCR-based identification of methicillin–resistant Staphylococcus aureus strains and their antibiotic resistance profiles

Pournajaf, Abazar; Ardebili, Abdollah; Goudarzi, Leyla; Khodabandeh, Mahmoud; Narimani, T; Abbaszadeh, Hassan

doi:10.12980/apjtb.4.2014c423

Cited by 82 publications

(63 citation statements)

References 18 publications

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“…PCR based identification of methicillin resistant S. aureus strains and their antibiotic resistance patterns were evaluated by Pournajaf et al (2014). Among antibiotics used in their study, penicillin showed the least activity against S. aureus and vancomycin was the most effective.…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of antibiotic resistance genes in multidrug-resistant Staphylococcus aureus isolates from dog dental plaque

Gharajalar¹,

Onsori²

2019

BJVM

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Multidrug resistant Staphylococcus aureus strains are a major health care problem both in humans and animals. In this work we described three multiplex PCR assays for detection of clinically relevant antibiotic resistance genes in S. aureus isolated from dog dental plaques. Thirty dental plaque samples were collected; then cultural, biochemical and molecular tests performed for isolation and identification of S. aureus from samples. The antibiotic susceptibility of the isolates were checked by Kirby Bauer disc diffusion method and the prevalence of antibiotic resistance genes determined using multiplex PCR assay. As a result S. aureus was isolated from 18 dog plaque samples. Fifteen of these isolates were resistant to penicillin. The mecA gene was more prevalent than blaZ among penicillin-resistant bacteria. Ten of the isolates were resistant to tetracycline. The percentage of tetM was higher than tetK among them. Also, 10 of the isolates were resistant to cefazolin among them bla TEM detected in higher rate than blaSHV and blaOXA-1. Hence multiplex PCR assay is a suitable method for detection of antibiotic resistance patterns of S. aureus isolates.

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Section: Discussionmentioning

confidence: 99%

“…There are several phenotypic methods for detection of microorganisms' antimicrobial susceptibility patterns, which however are not discriminating enough. So, it is essential to use more rapid and accurate methods like PCR for identification of staphylococci and their resistance patterns (Pournajaf et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of antibiotic resistance genes in multidrug-resistant Staphylococcus aureus isolates from dog dental plaque

Gharajalar¹,

Onsori²

2019

BJVM

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“…Two tests were used to identify the type of bacteria namely; coagulase and catalase [14]. The identification of S. aureus suspicious grown colonies was based on gram staining and standard biochemical reactions, including catalase and coagulase [15].…”

Section: Methodsmentioning

confidence: 99%

Conquering methicillin resistant Staphylococcus aureus: perspectives and treatment options

Rashid

Qureshi

et al. 2015

Int Arch Med

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Exploring optimal cure of Methicillin Resistant Staphylococcus aureus (MRSA), has been consistently challenged by clinical physicians and microbiologist. The fatal bacterial infection, Methicillin Resistant Staphylococcus aureus (MRSA), shows unique resistance; when selected drugs are tested on human patients. If holistic approach is overlooked, less possibilities are left for medication to curtail this lethal toxicity. If single range of drug treatment is exercised only, the associated complications and consequences of infections may proliferate beyond threshold levels. The research article is an outcome from primary microbial data collection (from urine and blood) in hospital. The focus of experiment is to scrutinize the highest risk vulnerable hospital area including sampled fluid. The preventive and therapeutic measures are discussed along with multiple substitutes of medication with prudent selection to achieve targeted and optimum outcome.

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“…The multiplex PCR method is a rapid, accurate, and useful assay to detect the mecA gene in MRSA strains, particularly in a hospital setting (25).…”

Section: Multiplex Pcr Assay For Assignment Of the Mec Element Typementioning

confidence: 99%

A Frequency and Molecular Typing Study of Methicillin-Resistant Staphylococcus aureus Isolates in Teaching Hospitals in Shahrekord, SouthWestern Iran

Taghaddosi

Gholipour

Zeraatpisheh

et al. 2016

Jundishapur J Microbiol

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PCR-based identification of methicillin–resistant Staphylococcus aureus strains and their antibiotic resistance profiles

Abstract: This study is suggestive that the PCR for detection of mecA gene is a fast, accurate and valuable diagnostic tool, particularly in hospitals in areas where methicillin-resistant S. aureus is endemic.

Cited by 82 publications

References 18 publications

Molecular detection of antibiotic resistance genes in multidrug-resistant Staphylococcus aureus isolates from dog dental plaque

Molecular detection of antibiotic resistance genes in multidrug-resistant Staphylococcus aureus isolates from dog dental plaque

Conquering methicillin resistant Staphylococcus aureus: perspectives and treatment options

A Frequency and Molecular Typing Study of Methicillin-Resistant Staphylococcus aureus Isolates in Teaching Hospitals in Shahrekord, SouthWestern Iran

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