PCR-Based Genetic Markers for Detection and Infection Frequency Analysis of the Biocontrol FungusChondrostereum purpureumon Sitka Alder and Trembling Aspen

Becker, Elisa; Ball, L. Andrew; Hintz, William E.

doi:10.1006/bcon.1999.0693

Cited by 33 publications

(34 citation statements)

References 22 publications

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“…SCAR markers were originally developed for markerassisted selection in plant breeding programs (Paran and Michelmore 1993;Lahogue et al 1998). They have proven to be of great value for identifying organisms in complex samples (Toth et al 1998;Becker et al 1999). SCAR markers were obtained to identify truffles of economic value at the species level.…”

Section: Scar Markers To Identify Laccaria Bicolor S238nmentioning

confidence: 99%

“…SCAR markers have been of great value already for identifying organisms in complex samples, e.g. pathogenic microorganisms or fungi used for biocontrol in plants (Toth et al 1998;Becker et al 1999). In particular, SCAR markers enable the identification of truffle species in roots of inoculated seedlings (Gandeboeuf et al 1997;Bertini et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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SCAR markers to detect mycorrhizas of an American Laccaria bicolor strain inoculated in European Douglas-fir plantations

et al. 2002

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The American strain S238N of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton has been used to inoculate Douglas-fir [Pseudotsuga menziesii (Mir.) Franco] plantations in France over the last two decades. Laccaria fruit bodies are scarce in mature plantations, which precludes further assessment of its persistence by fruit body surveys. Our objective was to develop new markers to identify this strain and its eventual non-fruiting progeny on root tips. We converted nine random amplified polymorphic DNA markers into sequence characterized amplified region (SCAR) markers. Two of these SCAR markers enabled us to detect S238N on roots of seedlings and mature trees. No amplification of non-fungal (host plant, bacterial, etc.) DNA was observed. Moreover, both SCARs were amplified from Laccaria-like mycorrhizas in a Douglas-fir plantation inoculated 14 years ago, demonstrating the long-term persistence of the inoculant strain. We also obtained a SCAR marker to detect one strain of European origin (L. bicolor 81306), indicating that SCARs are potential markers to type the naturally occurring genets. Thus, SCAR markers are of great value in studying the persistence of inoculant strains and the effects on local populations of introducing foreign strains.

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Section: Scar Markers To Identify Laccaria Bicolor S238nmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

SCAR markers to detect mycorrhizas of an American Laccaria bicolor strain inoculated in European Douglas-fir plantations

et al. 2002

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“…Genomic DNA of C. purpureum isolate 2139 was amplified using APD13F + R primers (Becker et al, 1999a). A 1 ll (1.0 lg/ll) sample of the amplified fragments was shotgun cloned into the pGEM-T vector, using the protocols of the T-vector cloning system (Promega).…”

Section: Cloning Of Apd13 Bands and Sequence Analysismentioning

confidence: 99%

“…This program required a reliable, molecular-based method of strain identification that permitted both species-and strain-specific identification of any C. purpureum isolates collected in the field. Genetic marker development is described in Becker et al (1999a). The method uses SCAR (sequence characterized amplified region) PCR primers to amplify a collection of variable-length fragments from the target genomic DNA of any C. purpureum strain and provides a unique, reproducible polymorphic banding pattern for each genetic individual.…”

Section: Introductionmentioning

confidence: 99%

A retrotransposon-like element and its occurrence in British Columbia populations of Chondrostereum purpureum

Becker

Bastide

Hintz

2004

Fungal Genetics and Biology

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“…Another approach is to track the BCA based on sequence-characterised amplified region (SCAR) PCR methodology. This approach has been applied to monitor potential bacterial biocontrol strains of for example the Pseudomonas genus, such as Pseudomonas fluorescens (Pujol et al 2005) and Pseudomonas brassicacearum (Holmberg et al 2009) but also various fungi such as Chondrostereum purpureum (Becker et al 1999), Gliocladium catenulatum (Paavanen-Huhtala et al 2000), Colletotrichum coccodes (Dauch et al 2003), Pichia anomala (De Clercq et al 2003), Beauvaria bassiana (Castrillo et al 2003), Rhizoctonia solani (Grosch et al 2007), among them several Trichoderma spp. (Abbasi et al 1999;Hermosa et al 2001;Dodd et al 2004;Rubio et al 2005;Cordier et al 2007;Savazzini et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples

et al. 2011

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Several strains from the genus Trichoderma (Ascomycetes, Hypocreales) are commercially used as biocontrol agents, e.g. in formulations containing the two Trichoderma strains IMI206039 (Hypocrea parapilulifera B.S. Lu, Druzhinina & Samuels) and IMI206040 (T. atroviride P. Karst). To quantify the presence of the two isolates after application, we developed primers for SCAR markers (Sequence-Characterised Amplified Region). In order to quantify both fungal strains simultaneously, we also designed fluorophore-labelled probes distinguishing the two strains, to be used in combination with the SCAR primers. In incubations of two different soils, artificially inoculated and maintained under controlled conditions, the quantification through amplification with the SCAR markers in qPCR and through colony-forming units from plate counting correlated well. Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish, either on the golf green or in the surrounding area.

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PCR-Based Genetic Markers for Detection and Infection Frequency Analysis of the Biocontrol FungusChondrostereum purpureumon Sitka Alder and Trembling Aspen

Cited by 33 publications

References 22 publications

SCAR markers to detect mycorrhizas of an American Laccaria bicolor strain inoculated in European Douglas-fir plantations

SCAR markers to detect mycorrhizas of an American Laccaria bicolor strain inoculated in European Douglas-fir plantations

A retrotransposon-like element and its occurrence in British Columbia populations of Chondrostereum purpureum

Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples

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