PCR-Based Detection of Traces of Potentially Allergenic Soybean (&lt;i&gt;Glycine max&lt;/i&gt;) in Food Matrices

Wang, Bang Ping; Li, Zhen Xing; Pawar, R. A.; Wang, Xiao Fei; Lin, Hong

doi:10.4028/www.scientific.net/amr.343-344.618

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…Consequently, they were applied in multiplex PCR analysis. The detected allergen-specific PCR fragments can be considered as novel DNA markers as they are distinguished from the allergen DNA markers described previously for soybean and GMO [ 9 , 19 , 20 , 21 ]. These DNA markers are 86 bp and 98 bp amplicons for Gly m Bd 28K; 129 bp, 118 bp, and 122 bp amplicons for Gly m Bd 30K; 135 bp amplicon for lectin as well as 114 bp and 172 bp amplicons for epsps gene.…”

Section: Discussionmentioning

confidence: 99%

“…The main advantage of the PCR technique is its specificity, reliability, and rapidity allowing the detection of trace amounts of allergens in processed products. The end-point and real-time PCR methods have been reported for the detection of soybean allergens, namely Gly m Bd 28K [ 19 ], Gly m Bd 30K [ 20 ], lectin [ 21 ]. The comparison of ELISA and PCR methods using soya food products showed that the sandwich ELISA had the lowest detection limit of 0.05 ppm, but only identified soy in five out of the eight products.…”

Section: Introductionmentioning

confidence: 99%

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Development of Multiplex PCR Coupled DNA Chip Technology for Assessment of Endogenous and Exogenous Allergens in GM Soybean

Kutateladze¹,

Bitskinashvili

Sapojnikova

et al. 2021

Biosensors

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Allergenicity assessment of transgenic plants and foods is important for food safety, labeling regulations, and health protection. The aim of this study was to develop an effective multi-allergen diagnostic approach for transgenic soybean assessment. For this purpose, multiplex polymerase chain reaction (PCR) coupled with DNA chip technology was employed. The study was focused on the herbicide-resistant Roundup Ready soya (RRS) using a set of certified reference materials consisting of 0, 0.1%, 0.5%, and 10% RRS. Technically, the procedure included design of PCR primers and probes; genomic DNA extraction; development of uniplex and multiplex PCR systems; DNA analysis by agarose gel electrophoresis; microarray development, hybridization, and scanning. The use of the asymmetric multiplex PCR method is shown to be very efficient for DNA hybridization with biochip probes. We demonstrate that newly developed fourplex PCR methods coupled with DNA-biochips enable simultaneous identification of three major endogenous allergens, namely, Gly m Bd 28K, Gly m Bd 30K, and lectin, as well as exogenous 5-enolppyruvyl shikimate-phosphate synthase (epsps) expressed in herbicide-resistant roundup ready GMOs. The approach developed in this study can be used for accurate, cheap, and fast testing of food allergens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Multiplex PCR Coupled DNA Chip Technology for Assessment of Endogenous and Exogenous Allergens in GM Soybean

Kutateladze¹,

Bitskinashvili

Sapojnikova

et al. 2021

Biosensors

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show abstract

“…Genetic markers for identifying Gly m Bd 28 K free cultivars have not been applied so far. However, both cDNA sequence of the allergen gene (TSUJI et al, 2001) as well as a PCR primer for allergen detection (WANG et al, 2012) have been published which could support the development of an efficient marker system for selection purposes.…”

Section: Gly M Bd 28 Kmentioning

confidence: 99%

From proteomics to ionomics: Soybean genetic improvement for better food safety

2018

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Soybean is a major protein and oilseed crop for food and livestock feed production, which is increasingly utilized in the food industry due to its favorable protein content and a superior overall seed composition with a high nutritional value. However, some of the soybean seed components have the potential to reduce the value of soy-food products as they are posing different food safety risks. Therefore, the objective of the present review was to evaluate options of soybean genetic improvement for the development of food-grade soybeans with a focus on food safety traits. To date, useful genetic variation in soybean germplasm collections and breeding materials has been described for protein components such as allergens or anti-nutritional factors, for fatty acid composition relevant to food safety, and for toxic heavy metal accumulation. Due to the progress in genomic research, genetic markers are available for assisting the introgression of major food safety traits into breeding populations, and the genetic mechanisms behind particular food safety traits have been clarified. Moreover, analytical methods from the fields of proteomics or ionomics are helpful for validating selection response and for monitoring quality features across genotypes. As consumer demand for food safety is steadily increasing, plant breeding approaches are gaining in importance as they can provide high-quality soybean raw materials to the food industry. For implementing better food safety on the consumer level, however, it appears that coordinated action between plant breeding and genetic research, food processing and marketing of products needs to be developed.

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PCR-Based Detection of Traces of Potentially Allergenic Soybean (<i>Glycine max</i>) in Food Matrices

Cited by 2 publications

References 16 publications

Development of Multiplex PCR Coupled DNA Chip Technology for Assessment of Endogenous and Exogenous Allergens in GM Soybean

Development of Multiplex PCR Coupled DNA Chip Technology for Assessment of Endogenous and Exogenous Allergens in GM Soybean

From proteomics to ionomics: Soybean genetic improvement for better food safety

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