PCR-based assay to detect the EPSPS TAP-IVS substitution in Amaranthus hybridus

Mathioni, Sandra M.; Oliveira, C.; Lemes, Lúcio N.; Ozório, Eduardo G.; Rosa, Daniel Dias

doi:10.51694/advweedsci/2022;40:amaranthus003

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…The chromatogram of the CAMAQ-R biotype presents double peaks in the first nucleotide of the TCA 106 codon (Figure 2A) that were also verified in the different self-fertilized generations G1 to G2, showing that the locus was not heterozygotes (Supplementary Figure 4). Similarly, the double peaks were found in a previous study with A. hybridus in Brazil (Mathioni et al 2022). The analysis of relative genomic EPSPS gene copy number indicates that there is more than one EPSPS locus in ARRGR-R, SAOJER-R, and CAMAQ-R biotypes (Figure 2B).…”

Section: Target-site Mutation and Increase In Copy Numbersupporting

confidence: 84%

“…In 2018, a population of A. hybridus was found in Brazil with multiple resistance to glyphosate and chlorimuron (Heap 2023). However, in those cases, the mechanism(s) of resistance has not been characterized (Mathioni et al 2022; Resende et al 2020). Recently, a study identified and characterized multiple resistance to ALS and EPSPS inhibitors for the first time in populations of A. hybridus in Argentina (García et al 2020).…”

Section: Resultsmentioning

confidence: 99%

“…In Paraguay, the replacement of Thr-102-Ile and Pro-106-Thr (TIPT) was identified in greater beggarticks ( Bidens subalternans DC) and confers high-level resistance to glyphosate (Takano et al 2020). The triple mutation (TAP-IVS) was identified in biotypes of A. hybridus in Argentina and Brazil (García et al 2020; Mathioni et al 2022; Perotti et al 2019). Due to the difficulty of occurrence of triple mutation in the same gene, because it is a rare event, it is suggested that the resistant biotypes existing in southern Brazil are due to the dissemination of seeds of resistant biotypes from Argentina.…”

Section: Resultsmentioning

confidence: 99%

“…However, the mechanism of resistance to ALS-inhibiting herbicides in biotypes from Brazil has not been characterized. Interestingly, glyphosate-resistant biotypes from southern Brazil have the same triple amino acid substitution Thr-102-IIe, Val-103-Ala, and Pro-106-Ser (TAP-IVS) (Mathioni et al 2022) that was reported for the first time in Argentina (Perotti et al 2019). However, limited information is available about the dispersion of the EPSPS triple mutation in Brazil populations.…”

Section: Introductionmentioning

confidence: 99%

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Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

Sulzbach,

Turra,

Cutti

et al. 2023

Weed Sci

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The presence of glyphosate-resistant smooth pigweed (Amaranthus hybridus L.) biotypes has increased in southern Brazil in the last years, presenting the triple amino acid substitution TAP-IVS in the 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), as previously found in Argentina. Some of these biotypes have morphological characteristics of smooth pigweed and redroot amaranth (Amaranthus retroflexus L.). The present study aimed to identify, through molecular markers, the herbicide-resistant species of Amaranthus from Brazil that have the TAP-IVS substitution and to analyze the occurrence of pollen-mediated gene flow (PMGF) as the source of TAP-IVS substitution in these biotypes. Six biotypes were evaluated by the internal transcribed spacer (ITS) sequences, of which two (AMACHY-S and CAMAQ-R) were molecularly classified as A. hybridus, and four (AMACRET-S, AMACVI-S, ARRGR-R, and SAOJER-R) were unclassified. Interestingly, all the glyphosate-resistant biotypes (ARRGR-R, SAOJER-R, and CAMAQ-R) had the TAP-IVS substitution, and an increase in EPSPS relative copy number; however, only CAMAQ-R was confirmed as A. hybridus. Although the biotypes ARRGR-R and SAOJER-R are closely related to A. hybridus and green pigweed (Amaranthus powellii S. Watson), their species identity could not be resolved. The biotype SAOJER-R also was resistant to ALS-inhibiting herbicides due to a tryptophan to leucine substitution at position 574 in acetolactate synthase (ALS). The evaluation of 119,746 seedlings in an intraspecific hybridization study of A. hybridus indicated an outcrossing frequency of 0.09%. In contrast, an absence of interspecific hybridization (A. hybridus × unclassified biotype – AMACVI-S) was found after screening 111,429 offspring. Unclassified biotypes might be derived from one or more ancient hybridization events and subsequently evolved the triple mutation independently. Alternatively, such biotypes could have evolved from recent hybridization events, which occur at a frequency below the level of detection in our study.

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Section: Target-site Mutation and Increase In Copy Numbersupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

Sulzbach,

Turra,

Cutti

et al. 2023

Weed Sci

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“…As a result, the mutated EPSPS gene at the Pro-106 position becomes less sensitive to glyphosate, allowing the EPSPS to maintain proper functionality in the route of shikimic acid in R plants when the herbicide is present. The selection pressure with glyphosate should be reduced in the R S. laxum population that presents the mutation Pro106Ser, and in other putative resistant populations, there is a great risk of double (TIPS and TIPT) and triple (TAP-IVS) mutations [ 25 , 42 , 43 , 44 ], which increase the degree of glyphosate resistance, being present in these species.…”

Section: Discussionmentioning

confidence: 99%

Divergence in Glyphosate Susceptibility between Steinchisma laxum Populations Involves a Pro106Ser Mutation

Hoyos,

Plaza,

Palma-Bautista

et al. 2023

Plants

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The characterization of the mechanisms conferring resistance to herbicides in weeds is essential for developing effective management programs. This study was focused on characterizing the resistance level and the main mechanisms that confer resistance to glyphosate in a resistant (R) Steinchisma laxum population collected in a Colombian rice field in 2020. The R population exhibited 11.2 times higher resistance compared to a susceptible (S) population. Non-target site resistance (NTSR) mechanisms that reduced absorption and impaired translocation and glyphosate metabolism were not involved in the resistance to glyphosate in the R population. Evaluating the target site resistance mechanisms by means of enzymatic activity assays and EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene sequencing, the mutation Pro106Ser was found in R plants of S. laxum. These findings are crucial for managing the spread of S. laxum resistance in Colombia. To effectively control S. laxum in the future, it is imperative that farmers use herbicides with different mechanisms of action in addition to glyphosate and adopt Integrate Management Programs to control weeds in rice fields of the central valleys of Colombia.

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Resistance of Amaranthus hybridus to glyphosate: detection, mechanisms involved and alternatives for integrated management

Lamego,

Nachtigall,

Machado

et al. 2024

Advances in Weed Science

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PCR-based assay to detect the EPSPS TAP-IVS substitution in Amaranthus hybridus

Cited by 3 publications

References 27 publications

Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

Smooth pigweed (Amaranthus hybridus) and unresolved Amaranthus spp. from Brazil resistant to glyphosate exhibit the EPSPS TAP-IVS substitution

Divergence in Glyphosate Susceptibility between Steinchisma laxum Populations Involves a Pro106Ser Mutation

Resistance of Amaranthus hybridus to glyphosate: detection, mechanisms involved and alternatives for integrated management

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