PCR analysis of different ladybird species (Coleoptera, Coccinellidae) with universal RAPD primers

Using RAPD PCR Method for Studying DNA Polymorphism of Agricultural Importance Arthropods

“…In this connect researchers should pay particular attention to the preliminary testing of RAPD primers. Choice of primers or use of only reproducible DNA fragments is the basis of effective and successful RAPD analysis of populations, closely related species, and even evaluation of interspecific genetic polymorphism [5][6][7].…”

“…In this case it is necessary to pick up RAPD primers at the same time high-specific to different types of insects. So, for example, we selected the primers suitable for the phylogenetic analysis of Coccinellidae family (OPA02 and OPA07) [7].…”