PCR-amplified microsatellites as markers in plant genetics

Morgante, Michèle; Olivieri, A. M.

doi:10.1111/j.1365-313x.1993.tb00020.x

Cited by 636 publications

(332 citation statements)

References 29 publications

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“…Simple sequence repeats (SSRs) (Morgante and Olivieri 1993), also known as microsatellites, are very powerful and informative markers for fingerprinting genotypes and determining genetic relationships, due to their abundance, co-dominant status, high reproducibility (Wani et al 2010), and high polymorphism (Singh et al 2008).…”

Section: Peak Of Molecular Studies With Ssrs From the Late 2000smentioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

153

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Persian or English walnut (Juglans regia L.), the walnut species cultivated for nut production, is one of the oldest food sources known. Persian walnuts, native to the mountain valleys of Central Asia, are grown worldwide in temperate areas. World production exceeds three million tons since 2012, mostly provided by China, the USA, and Iran. Despite very ancient culture of walnut species (Juglans spp.), breeding actually started in the twentieth century. Using a range of methodologies, from morphological markers to the most recent advances in genome analysis, many genetic studies of walnut have been conducted during the past 30 years, including examination of diversity, determination of relationships within or among germplasm collections and populations, phylogenetic and origin elucidation, genetic map construction, and biotic or abiotic stress investigations. The genetic improvement of walnut has undergone great evolution. The producing countries of the Middle East have widely studied morphological characteristics of walnut. The USA and France, for example, are behind important cultivar releases such as BChandler^and BFranquette.^Finally, genomics represents a major breakthrough in walnut improvement, in particular by recent sequencing of both chloroplast and nuclear genomes. This review summarizes worldwide molecular and Bomics^studies and gives an overview of the main walnut breeding programs.

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Section: Peak Of Molecular Studies With Ssrs From the Late 2000smentioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

153

View full text Add to dashboard Cite

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“…These markers are polymorphic, abundant in eukaryotic organisms and well distributed throughout the genome (Tautz 1989;Morgante and Olivieri 1993). The SSRs are most suitable for rice because of their reproducibility, multiallelic nature, hypervariablility, codominant inheritance, relative abundance, and genome-wide coverage (Powell et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Genetic Diversity Analysis of Rice (Oryza sativaL.) Using SSR Markers

Rahmana

Rasaulc

Hossain

et al. 2012

Journal of Crop Improvement

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Assessment of genetic diversity and molecular characterization among elite rice varieties of (RM10713, RM279, RM424, RM6266, RM1155, RM289, RM20224 and RM5371) were identified that produced specific alleles only in the aromatic rice varieties and were useful for varietal identification and DNA fingerprinting of these aromatic rice varieties.The findings of this study sould be useful for varietal identification and could help in background selection in backcross breeding programs.

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“…This therefore implies that many of the indel-type markers developed in this study contained non-SSR indels. Generally, SSR sites are highly polymorphic, even within a species, and are often used for genotyping at the individual level (Morgante and Olivieri 1993), although several SSR markers showing species specificity have been reported (Momotaz et al 2004). Festulolium cultivars are often bred as polyploids .…”

Section: Species Specificities Of the Markers Within Combinations Of mentioning

confidence: 99%

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

et al. 2009

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DNA markers able to distinguish species or genera with high specificity are valuable in the identification of introgressed regions in interspecific or intergeneric hybrids.Intergeneric hybridization between the genera of Lolium and Festuca, leading to the reciprocal introgression of chromosomal segments, can produce novel forage grasses with unique combinations of characteristics. To characterize Lolium/Festuca introgressions, novel PCR-based expression sequence tag (EST) markers were developed. These markers were designed around intronic regions which show higher polymorphism than exonic regions. Intronic regions of the grass genes were predicted from the sequenced rice genome. Two hundred and nine primer sets were designed from Lolium/Festuca ESTs that showed high similarity to unique rice genes dispersed uniformly throughout the rice genome. We selected 61 of these primer sets as insertion-deletion (indel)-type markers and 82 primer sets as cleaved amplified polymorphic sequence (CAPS) markers to distinguish between Lolium perenne and Festuca pratensis. Specificity of these markers to each species was evaluated by the genotyping of four cultivars and accessions (32 individuals) of L. perenne and F. pratensis respectively. Evaluation using specificity indices proposed in this study suggested that many indel-type markers had high species specificity to L. perenne and F. pratensis, including 15 markers completely specific to both species. Forty-nine of the CAPS markers completely distinguish between the two species at bulk level.Chromosome mapping of these markers using a Lolium/Festuca substitution line 3 revealed syntenic relationships between Lolium/Festuca and rice largely consistent with previous reports. This intron-based marker system that shows a high level of polymorphisms between species in combination with high species specificity will consequently be a valuable tool in Festulolium breeding. Keywordsintergeneric (interspecific) hybrid -intron polymorphismsinsertion-deletion (indel) -specificity to species -rice genome -comparative genomics

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PCR-amplified microsatellites as markers in plant genetics

Cited by 636 publications

References 29 publications

Walnut: past and future of genetic improvement

Walnut: past and future of genetic improvement

Molecular Characterization and Genetic Diversity Analysis of Rice (Oryza sativaL.) Using SSR Markers

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information

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