PCR Amplification of a Superoxide Dismutase Gene (pasod) from Psychrophilic Bacteria (KOPRI22215) without Genome Information

Expression and Purification of Recombinant Superoxide Dismutase (PaSOD) from Psychromonas arctica in Escherichia coli

“…20 Our group has previously reported the existence of SOD in P. arctica Abbreviations: SOD; superoxide dismutase, PCR; polymerase chain reaction, IPTG; isopropyl-β-D-1-thiogalactopyranoside, NBT; nitro blue tetrazolium, WST-1; 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (PaSOD), whose genomic sequence has not yet been elucidated. 18 We were able to identify the coding gene for sod from P. arctica using a combination of gradient PCR with homologous primers and LA PCR in vitro cloning technology. Overexpression of PaSOD in Escherichia coli and the purification of recombinant PaSOD were carried out to examine the functional role of PaSOD in cold adaptation.…”

“…NJ522 and Psychromonas arctica. [17][18][19] Recently, the first crystal structure of FeSOD from the cold-adapted and fish-pathogenic bacterium Aliivibrio salmonicida (AsFeSOD) was determined. 20 Our group has previously reported the existence of SOD in P. arctica Abbreviations: SOD; superoxide dismutase, PCR; polymerase chain reaction, IPTG; isopropyl-β-D-1-thiogalactopyranoside, NBT; nitro blue tetrazolium, WST-1; 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (PaSOD), whose genomic sequence has not yet been elucidated.…”