PcCLCg is involved in the accumulation of Cl− in shoots for osmotic adjustment and salinity resistance in the Cl−-tolerant xerophyte Pugionium cornutum

Cui, Yan-Nong; Lin, Zi‐Ru; Cai, Mei‐Mei; Liu, Rui-Wen; Wang, Suo-Min; Ma, Qing

doi:10.1007/s11104-023-05926-7

Cited by 5 publications

(8 citation statements)

References 50 publications

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“…Interestingly, our transcriptome data showed that the transcript of HKT1;4 was only detective in leaf sheaths of sweet sorghum, and moreover, its expression was upregulated after NaCl treatment for 6 h (Table S7), suggesting that HKT1;4 should also play a key role in Na + accumulation in leaf sheaths of sweet sorghum. The vacuolar sequestration of Cl − mediated by chloride channel CLC dominates the accumulation of Cl − in plant tissues [24,33]. In our transcriptome data, the expression of CLCc in leaf sheaths of sweet sorghum was upregulated after NaCl treatment for both 6 and 48 h (Figure 7A).…”

Section: The Genes Related To Ion Transport Play Key Roles In the Sal...mentioning

confidence: 85%

“…To confirm the reliability of RNA sequencing (RNA-seq) results, we randomly selected 20 genes in transcriptome data, and determined the relative expression levels of these genes using qRT-PCR method with a StepOnePlus Real-Time PCR Thermocycler (ABI PRISM 7500, USA) [33]. Sweet sorghum internal reference gene SbActin1 was used as a control.…”

Section: Validation Of Resultsmentioning

confidence: 99%

“…However, in this study, the expression of NHX1 and NHX2 in leaf blades of sweet sorghum was downregulated after NaCl treatment for 6 and 24 h (Table S9 and S10), suggesting that there might be other molecular components involved in vacuolar sequestration of Na + in leaf blades of sweet sorghum. In Arabidopsis and Pugionium cornutum, CLCg is proven to mediate vacuolar Cl − sequestration in shoots [33,35]. In our transcriptome data, two transcripts of CLCg (named SbCLCg-1 and SbCLCg-2) were identified in sweet sorghum, and the expression of SbCLCg-1 was upregulated, while the expression of SbCLCg-2 was downregulated in leaf blades after NaCl treatment for 48 h (Table S10).…”

Section: The Genes Related To Ion Transport Play Key Roles In the Sal...mentioning

confidence: 87%

“…After that, samples were incubated in 100 mM acetic acid at 90°C for 2 h, then Na + and K + contents in tissues were determined using a flame spectrophotometer (Model 410 Flame; Sherwood Scientific, Ltd., Cambridge, UK) according to Wang et al [46]. Samples were also incubated with deionized water at 100°C for 2 h, then tissue Cl − content was determined using a chloride analyzer (Model 926, Sherwood Scientific Ltd., Cambridge, UK) [33], and tissue NO3 − content was determined by the colorimetric method as described by Drechsler et al [47].…”

Section: Measurement Of Ion Contents In Tissuesmentioning

confidence: 99%

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Physiological and Transcriptional Analyses Provide Insight Into Maintaining Ion Homeostasis of Sweet Sorghum Under Salt Stress

Guo,

Nie,

et al. 2023

Preprint

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Sweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “Lvjuren” and determined its ion accumulation traits under NaCl treatments; then explored key genes involved in Na+, Cl−, K+ and NO3− transport using transcriptome profiling and qRT-PCR method. The results showed that the growth and photosynthesis of sweet sorghum were unaffected by 50 and 100 mM NaCl treatments, indicative of a strong tolerance of this species to salt stresses. Under NaCl treatments, sweet sorghum could efficiently exclude Na+ from shoots and accumulate Cl− in leaf sheaths to avoid their overaccumulation in leaf blades; meanwhile, it possessed a prominent ability to sustain NO3− homeostasis in leaf blades. Transcriptome profiling identified several differentially expressed genes associated with Na+, Cl−, K+ and NO3− transport in roots, leaf sheaths and leaf blades of sweet sorghum after 200 mM NaCl treatment for 6 and 48 h. Moreover, transcriptome data and qRT-PCR results indicated that HKT1;5, CLCc and NPF7.3-1 should be key genes involved in Na+ retention in roots, Cl− accumulation in leaf sheaths and maintenance of NO3− homeostasis in leaf blades, respectively. Many TFs were also identified after NaCl treatment, which should play important regulatory roles in salt tolerance of sweet sorghum. This work lays a preliminary foundation for clarifying the molecular basis underlying the adaptation of sweet sorghum to adverse environments.

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Section: The Genes Related To Ion Transport Play Key Roles In the Sal...mentioning

confidence: 85%

Section: Validation Of Resultsmentioning

confidence: 99%

Section: The Genes Related To Ion Transport Play Key Roles In the Sal...mentioning

confidence: 87%

Section: Measurement Of Ion Contents In Tissuesmentioning

confidence: 99%

See 2 more Smart Citations

Physiological and Transcriptional Analyses Provide Insight Into Maintaining Ion Homeostasis of Sweet Sorghum Under Salt Stress

Guo,

Nie,

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Interestingly, our transcriptome data showed that the transcript of HKT1;4 was only detected in leaf sheaths of sweet sorghum; moreover, its expression was upregulated after NaCl treatment for 6 h ( Table S7 ), suggesting that HKT1;4 should also play a key role in Na + accumulation in leaf sheaths of sweet sorghum. The vacuolar sequestration of Cl − mediated by chloride channel CLC dominates the accumulation of Cl − in plant tissues [ 39 , 40 ]. In our transcriptome data, the expression of CLCc encoding gene in leaf sheaths of sweet sorghum was upregulated after NaCl treatment for both 6 and 48 h ( Figure 7 A).…”

Section: Discussionmentioning

confidence: 99%

Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress

Guo,

Nie,

et al. 2023

IJMS

Self Cite

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Sweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “Lvjuren” and determined its ion accumulation traits under NaCl treatments; then, we explored key genes involved in Na+, Cl−, K+ and NO3− transport using transcriptome profiling and the qRT-PCR method. The results showed that growth and photosynthesis of sweet sorghum were unaffected by 50 and 100 mM NaCl treatments, indicative of a strong salt tolerance of this species. Under NaCl treatments, sweet sorghum could efficiently exclude Na+ from shoots and accumulate Cl− in leaf sheaths to avoid their overaccumulation in leaf blades; meanwhile, it possessed a prominent ability to sustain NO3− homeostasis in leaf blades. Transcriptome profiling identified several differentially expressed genes associated with Na+, Cl−, K+ and NO3− transport in roots, leaf sheaths and leaf blades after 200 mM NaCl treatment for 6 and 48 h. Moreover, transcriptome data and qRT-PCR results indicated that HKT1;5, CLCc and NPF7.3-1 should be key genes involved in Na+ retention in roots, Cl− accumulation in leaf sheaths and maintenance of NO3− homeostasis in leaf blades, respectively. Many TFs were also identified after NaCl treatment, which should play important regulatory roles in salt tolerance of sweet sorghum. In addition, GO analysis identified candidate genes involved in maintaining membrane stability and photosynthetic capacity under salt stresses. This work lays a preliminary foundation for clarifying the molecular basis underlying the adaptation of sweet sorghum to adverse environments.

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Integrated metabolomic and transcriptomic analyses reveal the roles of alanine, aspartate and glutamate metabolism and glutathione metabolism in response to salt stress in tomato

Liu,

Zheng,

et al. 2024

Scientia Horticulturae

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PcCLCg is involved in the accumulation of Cl− in shoots for osmotic adjustment and salinity resistance in the Cl−-tolerant xerophyte Pugionium cornutum

Cited by 5 publications

References 50 publications

Physiological and Transcriptional Analyses Provide Insight Into Maintaining Ion Homeostasis of Sweet Sorghum Under Salt Stress

Physiological and Transcriptional Analyses Provide Insight Into Maintaining Ion Homeostasis of Sweet Sorghum Under Salt Stress

Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress

Integrated metabolomic and transcriptomic analyses reveal the roles of alanine, aspartate and glutamate metabolism and glutathione metabolism in response to salt stress in tomato

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