PCC4, a new cell surface antigen common to multipotential embryonal carcinoma cells, spermatozoa, and mouse early embryos

Gachelin, Gabriel; Kemler, Rolf; Kelly, Françoise; Jacob, François

doi:10.1016/0012-1606(77)90365-7

Cited by 63 publications

(20 citation statements)

References 14 publications

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“…But, in contrast to other human and mouse teratocarcinoma cell lines (Artzt et al, 1973;Hogan et al, 1977;Holden et al, 1977), they had no detectable F9 antigen, which is known to be present in preimplantation mouse embryos from the two-cell stage on (Jacob, 1977). The PCC4 antigen (Gachelin et al, 1977) is expressed in mouse embryos only after the blastocyst stage and is coexpressed with the F9 antigen in many mouse teratocarcinoma cell lines. The detection of an antigen on PA 1 cells that cross-reacts with antiserum against the mouse PCC4 embryonic antigen suggests the presence of an analogous, evolutionally conserved determinant on human embryonic cells.…”

Section: Discussionmentioning

confidence: 79%

“…The F9 antigen (Artzt et al, 1973;Jacob, 1977), known to be expressed on both mouse embryonal carcinoma cells and some human teratocarcinoma cell lines (Hogan et ticular teratocarcinoma cell line Susa (Hogan et al, 1977) was used in parallel as a positive control in these studies. A second embryonic antigen, the PCC4 antigen (Gachelin et al, 1977) was detected on about 50% of the late-passage cells by indirect immunofluorescence.…”

Section: Surface Antigensmentioning

confidence: 99%

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Characterization of a human ovarian teratocarcinoma‐derived cell line

Zeuthen

Nörgaard

Avner

et al. 1980

Intl Journal of Cancer

149

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A cell line (PA I), derived from human ovarian teratocarcinoma cells, was obtained by culturing ascitic fluid cells from a patient with recurrence of malignant ovarian teratoma. During early passages the cultured cells showed a variable morphology, a long doubling time, and a low plating efficiency (2%). After about 50 passages in vitro, a cell population which was more homogeneous and resembled embryonal carcinoma cells were obtained. These cells had a shorter doubling time (26 h), and increased plating efficiency (77%). The early-passage cells were aneuploid (P 24) whereas the late-passage cells had a normal diploid karyotype with one balanced translocation between chromosomes No. 15 and No. 20 (P 224). Details of the karyotype suggest that the cells are heterozygous, i.e. derived from a stage before the first meiotic division. One of the two X chromosomes were inactive, and the cells expressed HLA antigens (A28 and B12), and beta 2-microglobulin. Expression of F9 antigen, characteristic of two-cell and later preimplantation embryos, was absent, while expression of PCC4 antigen, expressed also by blastocysts, was present. This finding suggests that the line might express some embryonic characteristics. The PA I cell line maintained in monolayer cultures showed several characteristics of malignant cells. The proportion of malignant cells increased with successive passages in vitro. The late-passage cells represented a fairly homogenous population of malignant cells similar to embryonal carcinoma cells. Late-passage PA I cells, when seeded under conditions that prevented attachment of cells to the substratum, formed embryoid bodies consisting of an inner core of cells similar to embryonal carcinoma cells, surrounded by a rind of endoderm-like cells. These two cell layers were separated by a basement membrane-like structure containing fibronectin. The core embryonal carcinoma cells expressed high alkaline phosphatase activity whereas the endoderm-like cells had low alkaline phosphatase activity. Embryoid bodies seeded on an adhesive substratum formed polycystic structures divided by layers of epithelial-like cells and containing extracellular fibrils similar to collagen type I or III. In these cultures, further limited differentiation into endoderm-like, epithelial-like cells and pigmented cells was observed. Morphological differenciation of undifferentiated PA I cells into endoderm-like cells in monolayer cultures could be obtained by treatment with BrdUrd or by plating in low serum concentration and at low density. Cells with characteristic fibrillar distribution of fibronectin and actin microfilament bundles were then observed, indicating formation of cells lacking properties of malignant cells. As indicated by these results, the PA I cell line, in spite of a limited capacity to differentiate in vitro, shares some of the properties of mouse teratocarcinoma cell lines and might therefore serve as a useful model for studies on some developmental mechanisms in human cells.

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Section: Discussionmentioning

confidence: 79%

Section: Surface Antigensmentioning

confidence: 99%

Characterization of a human ovarian teratocarcinoma‐derived cell line

Zeuthen

Nörgaard

Avner

et al. 1980

Intl Journal of Cancer

149

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“…The cells inside the blastocyst (inner cell mass, or ICM) give rise to all cells of the adult body and some extraembryonic tissues, while the trophectoderm gives rise to the outer layer of the placenta. Mouse EC cells express antigens and proteins that are similar to cells present in the ICM (Gachelin et al 1977;Solter and Knowles 1978), which led to the concept that EC cells are an in vitro counterpart of the pluripotent cells present in the ICM (Martin 1980). Indeed, some EC cell lines are able to contribute to various somatic cell types upon injection into mouse blastocysts (Brinster 1974).…”

Section: Embryonal Carcinoma (Ec) Sellsmentioning

confidence: 99%

Pluripotent stem cell lines

Yu¹,

Thomson²

2008

Genes Dev.

311

203

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The derivation of human embryonic stem cells 10 years ago ignited an explosion of public interest in stem cells, yet this achievement depended on prior decades of research on mouse embryonic carcinoma cells and embryonic stem cells. In turn, the recent derivation of mouse and human induced pluripotent stem cells depended on the prior studies on mouse and human embryonic stem cells. Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in vitro while maintaining the ability to differentiate into advanced derivatives of all three germ layers, features very useful for understanding the differentiation and function of human tissues, for drug screen and toxicity testing, and for cellular transplantation therapies. Here we review the family of pluripotent cell lines derived from early embryos and from germ cells, and compare them with the more recently described induced pluripotent stem cells.

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“…developmental time, site, murine strain) on the success of such grafts is described. The (Martin et Nicolas et al, 1975 (Wada et al, 1976 (Gachelin et al,1977) (Morello et al, 1978). Plus générale-ment, toutes les tentatives pour mettre en évidence la présence de H-2 à la surface du tératocarcinome se sont soldées par la négative (Artzt et Nicolas et al, 1975 ;Stern, Martin et Evans, 1976 ;Isa, 1976 ; revue dans Gachelin, 1978 (Segal, Levine et Khoury, 1979 (Stevens, 1964 ;Mintz, Cronmiller et Custer, 1978 …”

unclassified

Le tératocarcinome de la souris : origine et relation avec les cellules embryonnaires précoces

Condamine¹

1980

Reprod. Nutr. Dévelop.

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PCC4, a new cell surface antigen common to multipotential embryonal carcinoma cells, spermatozoa, and mouse early embryos

Cited by 63 publications

References 14 publications

Characterization of a human ovarian teratocarcinoma‐derived cell line

Characterization of a human ovarian teratocarcinoma‐derived cell line

Pluripotent stem cell lines

Le tératocarcinome de la souris : origine et relation avec les cellules embryonnaires précoces

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