PCAT-1: A Novel Oncogenic Long Non-Coding RNA in Human Cancers

Xiong, Tiefu; Li, Jianfa; Chen, Fangfang; Zhang, Fangting

doi:10.7150/ijbs.30970

Cited by 45 publications

(29 citation statements)

References 96 publications

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“…Recently, it is reported that lncRNAs play important roles in cellular functions, such as cell proliferation, apoptosis, invasion, gene regulation and chromatin remodeling 8. Moreover, large proportions of lncRNAs are involved in the cancer progression and metastasis 9, 10. The expression of lncRNAs is obviously tissue and cell type specific.…”

Section: Introductionmentioning

confidence: 99%

LncARSR sponges miR-129-5p to promote proliferation and metastasis of bladder cancer cells through increasing SOX4 expression

et al. 2020

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Emerging evidences have indicated that long non-coding RNAs (lncRNAs) are potential biomarkers, playing important roles in the development of cancer. LncRNA Activated in RCC with Sunitinib Resistance (lncARSR) is a novel lncRNA that functions as a potential biomarker and is involved in the progression of cancers. However, the clinical significance and molecular mechanism of lncARSR in bladder cancer (Bca) remains unknow. In this study, we discovered that lncARSR was significantly up-regulated in bladder cancer. In addition, increased expression of lncARSR was positively correlated with higher histological grade and larger tumor size. Further experiments demonstrated that suppression of lncARSR attenuated the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process of Bca cells. Mechanistically, lncARSR was mainly located in the cytoplasm and acted as a miRNA sponge to positively modulate the expression of Sex-determining region Y-related high-mobility-group box transcription factor 4 (SOX4) via sponging miR-129-5p and subsequently promoted the proliferation and metastasis of Bca cells, thus playing an oncogenic role in Bca pathogenesis. In conclusion, our study indicated that lncARSR plays a critical regulatory role in Bca cells and lncARSR may serve as a potential diagnostic biomarker and therapeutic target for bladder cancer.

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Section: Introductionmentioning

confidence: 99%

LncARSR sponges miR-129-5p to promote proliferation and metastasis of bladder cancer cells through increasing SOX4 expression

et al. 2020

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“…2 Mounting evidence exists on the important participation of non-coding RNAs (ncRNAs), especially long ncRNAs (lncRNAs) and microRNAs (miRNAs), in the initiation and progression in the variety of tumors. 3 , 4 , 5 , 6 Efficient biomarkers among them have been identified for cancer diagnosis and prognosis evaluation. Thus, insight into molecular biomarkers for CRC could considerably facilitate clinical diagnosis and treatment and promote the survival time of cancer patients.…”

Section: Introductionmentioning

confidence: 99%

LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer

et al. 2020

Molecular Therapy - Nucleic Acids

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A growing number of studies have focused on the involvement of non-coding RNAs (ncRNAs) in the internal ribosome entry site (IRES)-mediated translation in tumorigenesis; however, the underlying mechanisms in colorectal cancer (CRC) remain elusive. In this study, we show that LINC00473 (LNC473) exerted its functions as a tumor suppressor in promoting apoptotic protease-activating factor 1 ( APAF1 ) IRES activity through competitively sponging miR574-5p and miR15b-5p in CRC initiation and pathogenesis. Specifically, LNC473 and its downstream target APAF1 were significantly downregulated accompanied by upregulated miR574-5p and miR15b-5p in CRC cells and tissues, which had a significant prognostic impact on clinical outcomes in our CRC cohort (n = 157). Furthermore, ectopic LNC473 significantly sponged endogenous miR574-5p or miR15b-5p and thereby inhibited cell proliferation and colony formation capacity, and it accelerated cell apoptosis through activating the APAF1-CASP9-CASP3 pathway. Notably, LNC473 overexpression resulted in dramatic promotion of APAF1 IRES activity and translation, whereas rescue experiments confirmed the recovery by the existence of LNC473 and miR574/15b-5p. Mechanistically, LNC473 overexpression promoted IRES binding domain exposure and removed the constraints controlling from miR574-5p and miR15b-5p, and subsequently enhanced IRES-mediated APAF1 expression in vitro and in vivo . Therefore, our results uncover a novel LNC473-miR574/miR15b- APAF1 signaling axis, which provides new targets and crosstalk regulation mechanism for CRC prevention and treatment.

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“…Long non-coding RNAs (lncRNAs), are a group of RNAs which identified as more than 200 nucleotides in length with limited protein coding potential [5]. A large number of research studies have demonstrated that more than 16 000 lncRNAs in the human transcriptome were frequently spliced, polyadenylated, and principally transcribed by RNA polymerase II, which might play multitudinous roles in cancer cellular life circles as proliferation, metastasis, epithelial-mesenchymal transition (EMT), apoptosis, and differentiation [6].…”

Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA (LncRNA) UFC1/miR-34a Contributes to Proliferation and Migration in Breast Cancer

et al. 2019

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BackgroundAt present, a number of long non-coding RNAs (lncRNAs) have been realized as the critical regulators of breast cancers. Current evidence indicates that dysregulation of UFC1 contributes to the tumorigenesis and progression of various types of human cancer. However, the roles of UFC1 in breast cancer are still unclear.Material/MethodsFirstly, we measured the expression of UFC1 in breast cancer tissues and cells lines compared with corresponding controls. Then, cell functional assays were performed to determine the roles of UFC1 in breast cancer progression in vitro. Moreover, the correlation between UFC1 and miR-34a was determined by luciferase reporter assays. Further, the role of miR-34a in regulating biological function of breast cancer and its downstream target CXCL10 was applied by a series of functional assays.ResultsIn present study, we found that UFC1 was highly expressed in breast tissue and cells lines compared with normal tissues and cell lines. Silenced UFC1 suppressed multiple biological activities of breast cancer cells, which also functioned as a miR-34a sponge in breast cancer. Furthermore, over-expressing miR-34a could prominently suppress cell growth, invasion, migration and inducing apoptosis in breast cancer cells. In addition, we verified that miR-34a was a target of CXCL10 by bioinformatics analysis and luciferase reporter assay.ConclusionsLncRNA UFC1 regulated biological activity of breast cancer via miR-34a/CXCL10 axis, providing a novel diagnosis biomarker and potential therapeutic target for breast cancer.

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PCAT-1: A Novel Oncogenic Long Non-Coding RNA in Human Cancers

Cited by 45 publications

References 96 publications

LncARSR sponges miR-129-5p to promote proliferation and metastasis of bladder cancer cells through increasing SOX4 expression

LncARSR sponges miR-129-5p to promote proliferation and metastasis of bladder cancer cells through increasing SOX4 expression

LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer

Long Non-Coding RNA (LncRNA) UFC1/miR-34a Contributes to Proliferation and Migration in Breast Cancer

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