PCaPs, possible regulators of PtdInsP signals on plasma membrane

Kato, Mariko; Nagasaki-Takeuchi, Nahoko; Ide, Yuki; Tomioka, Rie; Maeshima, Masayoshi

doi:10.4161/psb.5.7.11825

Cited by 30 publications

(33 citation statements)

References 22 publications

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“…S2D). Hence, in agreement with previously published data, the N-terminal N23 domain and Gly-2 are required for the PM association of MAP18 (Kato et al, 2010(Kato et al, , 2013. In addition, PM-localized GFP-ROP2 is decreased significantly in MAP18DN23-overexpressing root hairs compared with MAP18-mCherry#1 (overexpression of full-length MAP18 with a similar expression level to MAP18DN23; Fig.…”

Section: The N23 Domain Is Necessary For Map18 To Interact With Rop2supporting

confidence: 81%

“…Additionally, the N23 domain also is sufficient for MAP18 binding to the Ca 2+ -calmodulin complex (Kato et al, 2013). However, the interaction of MAP18 with the Ca 2+ -calmodulin complex weakened the interaction between MAP18 and PtdInsPs (Kato et al, 2010). Previously published data have implicated the N23 domain of MAP18 in the regulation of root hair tip growth.…”

Section: G2amentioning

confidence: 98%

“…According to analyses of amino acid sequences from MAP18/PCaP2 and its homolog PCaP1/MDP25, MAP18 could be divided into highly conserved N-terminal (N23 domain) and C-terminal regions containing seven repeats of a VEEKK motif (Wang et al, 2007;Kato et al, 2010;Li et al, 2011;Zhu et al, 2013). It was reported that the N23 domain of MAP18 is responsible for its binding to PtdInsPs and is necessary for localization to the PM.…”

Section: G2amentioning

confidence: 99%

“…The second Gly residue (Gly-2) has been demonstrated as the site of N-myristoylation, which also is required for membrane anchoring. G-to-A mutation at this site abolished the PM localization of MAP18 (Kato et al, 2010). To examine whether the N23 domain also is required for the interaction between MAP18 and ROP2, we generated a truncated MAP18 mutant protein lacking the N-terminal 23 amino acids (MAP18DN23) and a mutant harboring a G2A point mutation (MAP18 G2A ).…”

Section: The N23 Domain Is Necessary For Map18 To Interact With Rop2mentioning

confidence: 99%

“…Either down-regulation or overexpression of MAP18 could lead to the disorganization of actin filaments in the apex and subapical regions of root hairs, resulting in aberrant root hair growth patterns (Zhang et al, 2015). Similarly, MAP18/PCaP2 has been shown to be expressed in root hairs and affects development (Kato et al, 2010(Kato et al, , 2013.…”

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confidence: 99%

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The Microtubule-Associated Protein MAP18 Affects ROP2 GTPase Activity during Root Hair Growth

et al. 2017

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Establishment and maintenance of the polar site are important for root hair tip growth. We previously reported that Arabidopsis (Arabidopsis thaliana) MICROTUBULE-ASSOCIATED PROTEIN18 (MAP18) functions in controlling the direction of pollen tube growth and root hair elongation. Additionally, the Rop GTPase ROP2 was reported as a positive regulator of both root hair initiation and tip growth in Arabidopsis. Both loss of function of ROP2 and knockdown of MAP18 lead to a decrease in root hair length, whereas overexpression of either MAP18 or ROP2 causes multiple tips or a branching hair phenotype. However, it is unclear whether MAP18 and ROP2 coordinately regulate root hair growth. In this study, we demonstrate that MAP18 and ROP2 interact genetically and functionally. MAP18 interacts physically with ROP2 in vitro and in vivo and preferentially binds to the inactive form of the ROP2 protein. MAP18 promotes ROP2 activity during root hair tip growth. Further investigation revealed that MAP18 competes with RhoGTPase GDP DISSOCIATION INHIBITOR1/SUPERCENTIPEDE1 for binding to ROP2, in turn affecting the localization of active ROP2 in the plasma membrane of the root hair tip. These results reveal a novel function of MAP18 in the regulation of ROP2 activation during root hair growth.

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Section: The N23 Domain Is Necessary For Map18 To Interact With Rop2supporting

confidence: 81%

Section: G2amentioning

confidence: 98%

Section: G2amentioning

confidence: 99%

Section: The N23 Domain Is Necessary For Map18 To Interact With Rop2mentioning

confidence: 99%

mentioning

confidence: 99%

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The Microtubule-Associated Protein MAP18 Affects ROP2 GTPase Activity during Root Hair Growth

et al. 2017

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Comparative proteomics and metallomics studies in Arabidopsis thaliana leaf tissues: Evaluation of the selenium addition in transgenic and nontransgenic plants using two‐dimensional difference gel electrophoresis and laser ablation imaging

et al. 2014

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The main goal of this work is to evaluate some differential protein species in transgenic (T) and nontransgenic (NT) Arabidopsis thaliana plants after their cultivation in the presence or absence of sodium selenite. The transgenic line was obtained through insertion of CaMV 35S controlling nptII gene. Comparative proteomics through 2D-DIGE is carried out in four different groups (NT × T; NT × Se-NT (where Se is selenium); Se-NT × Se-T, and T × Se-T). Although no differential proteins are achieved in the T × Se-T group, for the others, 68 differential proteins (by applying a regulation factor ≥1.5) are achieved, and 27 of them accurately characterized by ESI-MS/MS. These proteins are classified into metabolism, energy, signal transduction, disease/defense categories, and some of them are involved in the glycolysis pathway-Photosystems I and II and ROS combat. Additionally, laser ablation imaging is used for evaluating the Se and sulfur distribution in leaves of different groups, corroborating some results obtained and related to proteins involved in the glycolysis pathway. From these results, it is possible to conclude that the genetic modification also confers to the plant resistance to oxidative stress.

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PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization

et al. 2015

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PCaP2 plays a key role in maintaining the nucleus at a relatively fixed distance from the apex during root hair growth by modulating actin filaments. During root hair growth, the nucleus localizes at a relatively fixed distance from the apex. In Arabidopsis thaliana, the position of the nucleus is mainly dependent on the configuration of microfilaments (filamentous actin). However, the mechanisms underlying the regulation of actin dynamics and organization for nuclear positioning are largely unknown. In the present study, we demonstrated that plasma membrane-associated Ca(2+) binding protein 2 (PCaP2) influences the position of the nucleus during root hair growth. Abnormal expression of PCaP2 in pcap2 and PCaP2 over-expression plants led to the disorganization of actin filaments, rather than microtubules, in the apex and sub-apical regions of root hairs, which resulted in aberrant root hair growth patterns and misplaced nuclei. Analyses using a PCaP2 mutant protein revealed that actin-severing activity is essential for the function of PCaP2 in root hairs. We demonstrated that PCaP2 plays a key role in maintaining nuclear position in growing root hairs by modulating actin filaments.

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PCaPs, possible regulators of PtdInsP signals on plasma membrane

Abstract: Addendum to: Kato M, Nagasaki-Takeuchi N, Ide Y, Maeshima M. An Arabidopsis hydrophilic Ca 2+ -binding protein with a PEVK-rich domain, PCaP2, is associated with the plasma membrane and interacts with calmodulin and phosphatidylinositol phosphates.

Cited by 30 publications

References 22 publications

The Microtubule-Associated Protein MAP18 Affects ROP2 GTPase Activity during Root Hair Growth

The Microtubule-Associated Protein MAP18 Affects ROP2 GTPase Activity during Root Hair Growth

Comparative proteomics and metallomics studies in Arabidopsis thaliana leaf tissues: Evaluation of the selenium addition in transgenic and nontransgenic plants using two‐dimensional difference gel electrophoresis and laser ablation imaging

PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization

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