PC12 Cells as a Model of Neuronal Differentiation

Guroff, Gordon

doi:10.1007/978-1-4613-2473-7_8

Cited by 77 publications

(50 citation statements)

References 94 publications

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“…The PC12 cell line is a useful and widespread model for studying neuronal differentiation into sympathetic neurons and other neurobiochemical and neurobiological events [36]. H 2 O 2 has been extensively used as an inducer of oxidative stress in vitro model [3].…”

Section: Discussionmentioning

confidence: 99%

Attenuation of NF-κB and activation of Nrf2 signaling by 1,2,4-triazine derivatives, protects neuron-like PC12 cells against apoptosis

et al. 2010

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Oxidative stress has been implicated in the etiology of neurodegenerative diseases and aging. Indeed, accumulation of reactive oxygen species, such as hydrogen peroxide, generated by inflammatory cells, leads to oxidative stress, which may contribute to the neuronal degeneration observed in a wide variety of neurodegenerative disorders of the central nervous system, such as Alzheimer's disease. The present study indicates that H(2)O(2)-induced cell death can be inhibited in the presence of 1,2,4-triazine derivatives, as measured by MTT and caspase-3 activity. We further show that these compounds exert their protective effect by up-regulation of hemeoxygenase-1, glutamylcysteine synthetase, glutathione peroxidase and nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), while they inhibit NF-kappaB and decrease lipid peroxidation. It shows that there is a potential cross talk between NF-kappaB and Nrf2, an important cytoprotective transcription factor in the presence of these compounds. Moreover, in order for drugs to be effective in the treatment of neurodegenerative diseases, they must be capable of penetrating the blood-brain barrier, whereas more than 98% of all potential central nervous system drugs don't cross. Using a reliable model based on the artificial neural network indicated that these compounds satisfy this requirement.

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Section: Discussionmentioning

confidence: 99%

Attenuation of NF-κB and activation of Nrf2 signaling by 1,2,4-triazine derivatives, protects neuron-like PC12 cells against apoptosis

et al. 2010

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“…PC12 cells, an established cell line derived from a rat pheochromocytoma (Greene and Tischler, 1976) respond to treatment with NGF by acquiring many of the properties of sympathetic neurons (Greene and Tischler, 1982;Guroff, 1985) notably the ability to extend neurites on suitable substrata (Schubert and Whitlock, 1977;Greene, 1978;Vlodavsky et al, 1982;Heidemann et al, 1985), including laminin (Tomaselli et al, 1987;Turner et al, 1987). We showed (Turner et al, 1987) that PC1 2 cells attach by an "active" mechanism (Grinnell, 1978) to dishes coated with collagen and laminin; active attachment requires MgZ+ and is inhibited at low temperatures and in the presence of sodium azide.…”

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confidence: 94%

Identification of a cell-surface protein involved in PC12 cell- substratum adhesion and neurite outgrowth on laminin and collagen

1989

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On substrata coated with laminin or native collagen (Types I/III), PC12 cells employ an active adhesion mechanism (i.e., one inhibited at low temperature, by azide or in the absence of divalent cations) to attach and extend neurites; on substrata coated with wheat germ agglutinin (WGA) or polylysine, by contrast, PC12 cells attach via a passive mechanism and fail to extend neurites (Turner et al., 1987). This paper reports the isolation of 2 monoclonal antibodies (3A3 and 1B1) that promote retraction of neurites extended on laminin and collagen. In studies of initial cell attachment, 3A3 inhibited active attachment to laminin or collagen but not passive attachment to WGA or polylysine, whereas 1B1 inhibited both active and passive attachment. The more potent of the antibodies, 3A3, precipitates 2 radioactive protein bands (of approximately 185 and 125 kDa) from 1% Nonidet P-40 extracts of metabolically labeled PC12 cells. The properties of these proteins suggest that the antigen recognized by 3A3 is a member of the integrin family of matrix receptors. The other monoclonal antibody, 1B1, reacts with many PC12 proteins, including both bands precipitated by 3A3. The available data strongly suggest that an integrin with specificity for both laminin and collagen mediates PC12 adhesion to the substratum at both the cell body and the neurite growth cone.

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“…Since the derivation of a clonal cell line of rat pheochromocytoma, designated PC12 (Greene and Tischler, 1976), this line has become an established system for the study of neuronal differentiation in response to extracellular agonists, such as nerve growth factor (NGF) (reviewed in Greene and Tischler, 1982;Guroff, 1985;Fujita et al, 1989), sodium butyrate (Byrd and Alho, 1987), fibroblast growth factor (Togari et al, 1985), cyclic AMP (Richter-Landsberg and Jastorff, 1986;Katoh-Semba et al, 1987), cyclic AMP analogues (Peraldi and Vanobberghen, 1994;Young et al, 1994), and a number of other agents (reviewed in Fujita et al, 1989).…”

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confidence: 99%

Class III β-Tubulin isotype (β III) in the adrenal medulla: III. Differential expression of neuronal and glial antigens identifies two distinct populations of neuronal and glial-like (sustentacular) cells in the PC12 rat pheochromocytoma cell line maintained in a gelfoam matrix system

et al. 1998

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Background: The rat PC12 pheochromocytoma cell line provides an established system for the study of neuronal differentiation. To our knowledge, glial differentiation has not been reported in this cell line.Methods: We have studied, by immunohistochemistry and immunoblotting, the presence of neuronal cytoskeletal antigens [class III ␤-tubulin isotype (␤ III), microtubule associated proteins MAP2, MAP1B and tau, and different neurofilament (NF) protein components], and synaptophysin in comparison with the glial fibrillary acidic protein (GFAP) and S-100 protein in the PC12 cell line. In three different experiments, PC12 cells were maintained in a three-dimensional gelatin foam (Gelfoam) matrix system for up to 34 days with and without treatment with 1 mM dibutyryl cyclic (dc)AMP. Immunohistochemistry was performed on explants ranging from 2 to 32 days-in vitro, which were fixed in either Bouin's solution, 70% ethanol, or 10% neutral-buffered formalin and embedded in paraffin. Immunoblotting was performed on Gelfoam explants with a panel of antibodies against all aforementioned neuronal and glial markers. Additional immunoblot experiments using anti-GFAP and anti-␤ III monoclonal antibodies in cell suspensions and homogenates from PC12 monolayer cultures were carried out to compare growth conditions in relation to the expression of these proteins.

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PC12 Cells as a Model of Neuronal Differentiation

Cited by 77 publications

References 94 publications

Attenuation of NF-κB and activation of Nrf2 signaling by 1,2,4-triazine derivatives, protects neuron-like PC12 cells against apoptosis

Attenuation of NF-κB and activation of Nrf2 signaling by 1,2,4-triazine derivatives, protects neuron-like PC12 cells against apoptosis

Identification of a cell-surface protein involved in PC12 cell- substratum adhesion and neurite outgrowth on laminin and collagen

Class III β-Tubulin isotype (β III) in the adrenal medulla: III. Differential expression of neuronal and glial antigens identifies two distinct populations of neuronal and glial-like (sustentacular) cells in the PC12 rat pheochromocytoma cell line maintained in a gelfoam matrix system

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