PBN protects NP cells from AAPH-induced degenerative changes by inhibiting the ERK1/2 pathway

Zhou, Zhenggang; Wang, Yini; Liu, Haifei; Wang, Lu; Liu, Zonghan; Yuan, Hongchun; Liu, Lantao; Guo, Mingbo; Wang, Dechun

doi:10.1080/03008207.2020.1743697

Cited by 7 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, Exenatide regulates ECM anabolic balance and restores disc degeneration by inhibiting MAPK activation and its downstream AP-1 activity. As potent drugs inhibiting the ERK/MAPK pathway, PBN and Exenatide provide therapeutic targets for the treatment of DDD ( 123 , 124 ).…”

Section: Therapymentioning

confidence: 99%

The role of oxidative stress in intervertebral disc cellular senescence

et al. 2022

View full text Add to dashboard Cite

With the aggravation of social aging and the increase in work intensity, the prevalence of spinal degenerative diseases caused by intervertebral disc degeneration(IDD)has increased yearly, which has driven a heavy economic burden on patients and society. It is well known that IDD is associated with cell damage and degradation of the extracellular matrix. In recent years, it has been found that IDD is induced by various mechanisms (e.g., genetic, mechanical, and exposure). Increasing evidence shows that oxidative stress is a vital activation mechanism of IDD. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) could regulate matrix metabolism, proinflammatory phenotype, apoptosis, autophagy, and aging of intervertebral disc cells. However, up to now, our understanding of a series of pathophysiological mechanisms of oxidative stress involved in the occurrence, development, and treatment of IDD is still limited. In this review, we discussed the oxidative stress through its mechanisms in accelerating IDD and some antioxidant treatment measures for IDD.

show abstract

Section: Therapymentioning

confidence: 99%

The role of oxidative stress in intervertebral disc cellular senescence

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Gene chip technology has been used to detect gene expression profiling changes in the process of CESCs cartilage differentiation and to identify potential targets for the treatment of CESCs degeneration; however, whether these gene changes are directly involved in the change in CESCs function needs to be verified by cell and animal experiments 23 . Recent studies have shown that endogenous stem cells such as nucleus pulposus decrease in number and activity with ageing, suggesting that ageing of nucleus pulposus is very important in the degeneration of endogenous stem cells such as mesenchymal stem cell 24‐26 ; however, whether CESCs has similar phenomenon still needs further study. In this study, we performed bioinformatic analysis of http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15227 database using grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration, and we choose CHST3 and CSPG4 as the main targets in the following investigation.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

Guan

Sun

Zou

et al. 2021

J Cellular Molecular Medi

View full text Add to dashboard Cite

To investigate the regulatory effect of carbohydrate sulfotransferase 3 (CHST3) in cartilage endplate‐derived stem cells (CESCs) on the molecular mechanism of intervertebral disc degeneration after nucleus pulposus repair in rats. We performed GO and KEGG analysis of GSE15227 database to select the differential genes CHST3 and CSPG4 in grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration, IHC and WB to detect the protein profile of CHST3 and CSPG4, Co‐IP for the interaction between CHST3 and CSPG4. Then, immunofluorescence was applied to measure the level of CD90 and CD105, and flow cytometry indicated the level of CD73, CD90 and CD105 in CESCs. Next, Alizarin red staining, Alcian blue staining and TEM were performed to evaluate the effects of CESCs into osteoblasts and chondroblasts, respectively, CCK8 for the cell proliferation of osteoblasts and chondroblasts after induction for different times; cell cycle of osteoblasts or chondroblasts was measured by flow cytometry after induction, and WB for the measurement of specific biomarkers of OC and RUNX in osteoblasts and aggrecan, collagen II in chondroblasts. Finally, colony formation was applied to measure the cell proliferation of CESCs transfected with ov‐CHST3 or sh‐CHST3 when cocultured with bone marrow cells, WB for the protein expression of CHST3, CSPG4 and ELAVL1 in CSECs, transwell assay for the migration of CESCs to bone marrow cells, TEM image for the cellular characteristics of bone marrow cells, and WB for the protein profile of VCAN, VASP, NCAN and OFD1 in bone marrow cells. CHST3 and CSPG4 were differentially expressed and interacted in grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration; CD73, CD90 and CD105 were lowly expressed in CESCs, osteogenic or chondroblastic induction changed the characteristics, proliferation, cell cycle and specific biomarkers of osteoblasts and chondroblasts after 14 or 21 days,; CHST3 affected the cell proliferation, protein profile, migration and cellular features of cocultured CESCs or bone marrow cells. CHST3 overexpression promoted CESCs to regulate bone marrow cells through interaction with CSPG4 to repair the grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration.

show abstract

“…Han et al [ 74 ] found that H2O2-induced oxidative stress could trigger apoptosis and calcification in CEP cells through the MAPK/ERK signaling pathway. Zhou et al [ 75 ] found that AAPH, a small molecule widely used as a source of free radicals, could induce excess ROS production and apoptosis, in NPCs, by activating ERK.…”

Section: Introductionmentioning

confidence: 99%

IL-1β-mediated inflammatory responses in intervertebral disc degeneration: Mechanisms, signaling pathways, and therapeutic potential

Li,

Pan,

Xiao

et al. 2023

Heliyon

View full text Add to dashboard Cite

PBN protects NP cells from AAPH-induced degenerative changes by inhibiting the ERK1/2 pathway

Cited by 7 publications

References 41 publications

The role of oxidative stress in intervertebral disc cellular senescence

The role of oxidative stress in intervertebral disc cellular senescence

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

IL-1β-mediated inflammatory responses in intervertebral disc degeneration: Mechanisms, signaling pathways, and therapeutic potential

Contact Info

Product

Resources

About