PBMC MicroRNAs: Promising Biomarkers for the Differential Diagnosis of COVID-19 Patients with Abnormal Coagulation Indices

Kasho, Ammar Khalo Abass; Nahand, Javid Sadri; Salmaninejad, Arash; Mirzaei, Hamed; Moghoofei, Mohsen; Bazmani, Ahad; Aghbash, Parisa Shiri; Rasizadeh, Reyhaneh; Farsad-Akhtar, Nader; Baghi, Hossein Bannazadeh

doi:10.1007/s00284-023-03365-2

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…Moreover, Fisher's exact and Mann–Whitney U tests were utilized to determine the statistical difference between the two groups of COVID‐19 subjects. The correlation between the levels of ncRNAs with laboratory data and the clinical features of COVID‐19 subjects was determined via Spearman's correlation coefficient 48,49 …”

Section: Methodsmentioning

confidence: 99%

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Analyzing the expression pattern of the noncoding RNAs (HOTAIR, PVT‐1, XIST, H19, and miRNA‐34a) in PBMC samples of patients with COVID‐19, according to the disease severity in Iran during 2022–2023: A cross‐sectional study

Khanaliha,

Sadri Nahand,

Khatami

et al. 2024

Health Science Reports

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Background and aimsMicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) are well‐known types of noncoding RNAs (ncRNAs), which have been known as the key regulators of gene expression. They can play critical roles in viral infection by regulating the host immune response and interacting with genes in the viral genome. In this regard, ncRNAs can be employed as biomarkers for viral diseases. The current study aimed to evaluate peripheral blood mononuclear cell (PBMC) ncRNAs (lncRNAs‐homeobox C antisense intergenic RNA [HOTAIR], ‐H19, X‐inactive‐specific transcript [XIST], plasmacytoma variant translocation 1 [PVT‐1], and miR‐34a) as diagnostic biomarkers to differentiate severe COVID‐19 cases from mild ones.MethodsCandidate ncRNAs were selected according to previous studies and assessed by real‐time polymerase chain reaction in the PBMC samples of patients with severe coronavirus disease 2019 (COVID‐19) (n = 40), healthy subjects (n = 40), and mild COVID‐19 cases (n = 40). Furthermore, the diagnostic value of the selected ncRNAs was assessed by analyzing the receiver‐operating characteristic (ROC).ResultsThe results demonstrated that the expression pattern of the selected ncRNAs was significantly different between the studied groups. The levels of HOTAIR, XIST, and miR‐34a were remarkably overexpressed in the severe COVID‐19 group in comparison with the mild COVID‐19 group, and in return, the PVT‐1 levels were lower than in the mild COVID‐19 group. Interestingly, the XIST expression level in men with severe COVID‐19 was higher compared to women with mild COVID‐19. ROC results suggested that HOTAIR and PVT‐1 could serve as useful biomarkers for screening mild COVID‐19 from severe COVID‐19.ConclusionsOverall, different expression patterns of the selected ncRNAs and ROC curve results revealed that these factors can contribute to COVID‐19 pathogenicity and can be considered diagnostic markers of COVID‐19 severe outcomes.

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Section: Methodsmentioning

confidence: 99%

“…The correlation between the levels of ncRNAs with laboratory data and the clinical features of COVID-19 subjects was determined via Spearman's correlation coefficient. 48,49 3 | RESULTS…”

Section: Sequences Namementioning

confidence: 99%

Analyzing the expression pattern of the noncoding RNAs (HOTAIR, PVT‐1, XIST, H19, and miRNA‐34a) in PBMC samples of patients with COVID‐19, according to the disease severity in Iran during 2022–2023: A cross‐sectional study

Khanaliha,

Sadri Nahand,

Khatami

et al. 2024

Health Science Reports

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“…Despite the fact that the majority of miRNAs are found inside the cells, a significant number of them move outside of them and may be identified in body fluids [ 107 – 111 ]. These so-called circulating miRNAs are released into bodily fluids such as blood, urine, saliva, seminal fluid, and breast milk [ 30 , 34 ] as a result of tissue destruction, apoptosis, and necrosis [ 101 ], or by active transit in micro vesicles and exosomes, or by binding to a protein [ 112 , 113 ]. The existence of exogenous miRNA in the blood of healthy patients has also been challenged [ 114 , 115 ], with its origin attributed to bacteria, food, and fungus, predominantly from the gut [ 102 ].…”

Section: Biogenesis and Function Of Mirnasmentioning

confidence: 99%

“…Changes in miRNA levels in patients with respiratory disorders, diabetes, heart, and kidney failure are associated with the severity of COVID-19 [ 17 , 23 ] (Table 1 ). What is more, miRNAs have important functions in host antiviral responses in many viral infections as well, including herpes virus, polyomavirus, retroviruses, pestivirus, hepacivirus, and COVID-19 [ 27 , 30 ]. Likewise, miRNAs can have regulatory actions via multiple pathways in COVID-19 cases [ 5 ], and these small molecules might have significant roles in angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) regulation, which are the receptor and co-receptor of the virus respectively and are responsible for entry and membrane fusion of the virus, thus being a good choice to be inhibited as a therapy.…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2-associated organs failure and inflammation: a focus on the role of cellular and viral microRNAs

Rasizadeh

Aghbash

Nahand

et al. 2023

Virol J

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SARS-CoV-2 has been responsible for the recent pandemic all over the world, which has caused many complications. One of the hallmarks of SARS-CoV-2 infection is an induced immune dysregulation, in some cases resulting in cytokine storm syndrome, acute respiratory distress syndrome and many organs such as lungs, brain, and heart that are affected during the SARS-CoV-2 infection. Several physiological parameters are altered as a result of infection and cytokine storm. Among them, microRNAs (miRNAs) might reflect this poor condition since they play a significant role in immune cellular performance including inflammatory responses. Both host and viral-encoded miRNAs are crucial for the successful infection of SARS-CoV-2. For instance, dysregulation of miRNAs that modulate multiple genes expressed in COVID-19 patients with comorbidities (e.g., type 2 diabetes, and cerebrovascular disorders) could affect the severity of the disease. Therefore, altered expression levels of circulating miRNAs might be helpful to diagnose this illness and forecast whether a COVID-19 patient could develop a severe state of the disease. Moreover, a number of miRNAs could inhibit the expression of proteins, such as ACE2, TMPRSS2, spike, and Nsp12, involved in the life cycle of SARS-CoV-2. Accordingly, miRNAs represent potential biomarkers and therapeutic targets for this devastating viral disease. In the current study, we investigated modifications in miRNA expression and their influence on COVID-19 disease recovery, which may be employed as a therapy strategy to minimize COVID-19-related disorders.

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War or peace: Viruses and metastasis

Bayat,

Golestani,

Motlaghzadeh

et al. 2024

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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PBMC MicroRNAs: Promising Biomarkers for the Differential Diagnosis of COVID-19 Patients with Abnormal Coagulation Indices

Cited by 6 publications

References 55 publications

Analyzing the expression pattern of the noncoding RNAs (HOTAIR, PVT‐1, XIST, H19, and miRNA‐34a) in PBMC samples of patients with COVID‐19, according to the disease severity in Iran during 2022–2023: A cross‐sectional study

Analyzing the expression pattern of the noncoding RNAs (HOTAIR, PVT‐1, XIST, H19, and miRNA‐34a) in PBMC samples of patients with COVID‐19, according to the disease severity in Iran during 2022–2023: A cross‐sectional study

SARS-CoV-2-associated organs failure and inflammation: a focus on the role of cellular and viral microRNAs

War or peace: Viruses and metastasis

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