PBMC-Derived T Cells

Lozano‐Ojalvo, Daniel; López‐Fandiño, Rosina; López-Expósito, Iván

doi:10.1007/978-3-319-16104-4_16

Cited by 12 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strength and polarity of GABA-A R-activated chloride currents depend on the intracellular chloride concentration set by CCCs [ 17 ]. In murine T cells, only NKCC1 has been detected to date, but in human PBMCs (T cells constitute ~ 45–70% of PBMCs), NKCC1, KCC1, KCC3, KCC4 were detected [ 52 , 95 , 96 ]. One single subunit of GABA-B Rs (B1) was detected in human PBMCs and it remains enigmatic if functional GABA-B R homodimers can be formed in T cells [ 95 ].…”

Section: T Cellsmentioning

confidence: 99%

GABAergic signaling by cells of the immune system: more the rule than the exception

Bhandage

Barragán

2021

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Gamma-aminobutyric acid (GABA) is best known as an essential neurotransmitter in the evolved central nervous system (CNS) of vertebrates. However, GABA antedates the development of the CNS as a bioactive molecule in metabolism and stress-coupled responses of prokaryotes, invertebrates and plants. Here, we focus on the emerging findings of GABA signaling in the mammalian immune system. Recent reports show that mononuclear phagocytes and lymphocytes, for instance dendritic cells, microglia, T cells and NK cells, express a GABAergic signaling machinery. Mounting evidence shows that GABA receptor signaling impacts central immune functions, such as cell migration, cytokine secretion, immune cell activation and cytotoxic responses. Furthermore, the GABAergic signaling machinery of leukocytes is implicated in responses to microbial infection and is co-opted by protozoan parasites for colonization of the host. Peripheral GABA signaling is also implicated in inflammatory conditions and diseases, such as type 1 diabetes, rheumatoid arthritis and cancer cell metastasis. Adding to its role in neurotransmission, growing evidence shows that the non-proteinogenic amino acid GABA acts as an intercellular signaling molecule in the immune system and, as an interspecies signaling molecule in host–microbe interactions. Altogether, the data raise the assumption of conserved GABA signaling in a broad range of mammalian cells and diversification of function in the immune system.

show abstract

Section: T Cellsmentioning

confidence: 99%

GABAergic signaling by cells of the immune system: more the rule than the exception

Bhandage

Barragán

2021

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…T cells, classically described as the main mediators of beta cell destruction in T1D [ 2 ], are the most abundant cell type in peripheral blood mononuclear cells (PBMCs), making up approximately 45–70% [ 3 ]. The study of PBMCs provides important insight into the immunological status of patients with T1D [ 4 ], and circulating leukocytes are sensors of metabolic stress in patients and act as bioenergetic biomarkers [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial redox impairment and enhanced autophagy in peripheral blood mononuclear cells from type 1 diabetic patients

Canet¹,

Díaz-Pozo²,

Luna-Marco³

et al. 2022

Redox Biology

View full text Add to dashboard Cite

“…T cells comprise 45-70% of isolated mononuclear cells from human peripheral blood 55 . T cells that primarily rely on mitochondrial OXPHOS for ATP generation are resting naïve T cells, antigenspecific memory T cells, and FoxP3+ regulatory T cells, whereas proliferative helper (CD4+) and cytotoxic (CD8+) effector T cells utilize glycolysis to a higher extent [56][57][58][59] .…”

Section: Discussionmentioning

confidence: 99%

Peripheral blood mononuclear cells exhibit increased mitochondrial respiration after adjuvant chemo- and radiotherapy for early breast cancer

Christensen

Ribas

Buch-Larsen

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Adjuvant chemo- and radiotherapy cause cellular damage not only to cancerous but also to healthy dividing cells. Antineoplastic treatments have been shown to cause mitochondrial respiratory dysfunction in non-tumorous tissues, but the effects on circulating human peripheral blood mononuclear cells (PBMCs) remain unknown. Aim: We aimed to identify changes in mitochondrial respiration of PBMCs after adjuvant chemo- and radiotherapy in postmenopausal early breast cancer (EBC) patients and relate these to metabolic parameters of the patients. Methods: Twenty-three postmenopausal women diagnosed with EBC were examined before and shortly after chemotherapy treatment often administered in combination with radiotherapy (n=18). Respiration (O2 flux per million PBMCs) was assessed by high-resolution respirometry of intact and permeabilized PBMCs. Clinical metabolic characteristics were furthermore assessed. Results: Respiration of intact and permeabilized PBMCs from EBC patients was significantly increased after adjuvant chemo- and radiotherapy (p=6x10-5 and p=1x10-7, respectively). The oxygen flux attributed to specific mitochondrial complexes and respiratory states increased by 17-43% compared to before therapy commencement. Leukocyte counts (p=1x10-4), hemoglobin levels (p=0.0003), and HDL cholesterol (p=0.003) decreased while triglyceride (p=0.01) and LDL levels (p=0.02) increased after treatment suggesting a worsened metabolic state. None of the metabolic parameters correlated significantly with PBMC respiration. Conclusion: This study shows that mitochondrial respiration in circulating PBMCs is significantly increased after adjuvant chemo- and radiotherapy in postmenopausal EBC patients. The increase might be explained by a shift in PBMC subpopulation proportions towards cells relying on oxidative phosphorylation rather than glycolysis or a generally increased mitochondrial content in PBMCs. Both parameters might be influenced by therapy-induced changes to the bone marrow or vascular microenvironment wherein PBMCs differentiate and reside.

show abstract

PBMC-Derived T Cells

Cited by 12 publications

References 30 publications

GABAergic signaling by cells of the immune system: more the rule than the exception

GABAergic signaling by cells of the immune system: more the rule than the exception

Mitochondrial redox impairment and enhanced autophagy in peripheral blood mononuclear cells from type 1 diabetic patients

Peripheral blood mononuclear cells exhibit increased mitochondrial respiration after adjuvant chemo- and radiotherapy for early breast cancer

Contact Info

Product

Resources

About