PBIS-based system integrated with zinc–silver nanocomposite for the detection of Chikungunya virus

Sharma, Pramod; Hassan, Homa; Hasan, Mohd. Rahil; Fatima, Tarab; Mohan, Hari; Khanuja, Manika; Kaushik, Samander; Narang, Jagriti

doi:10.1016/j.biosx.2022.100303

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…After crushing the powder, nanorods were synthesized [ 34 , 35 ]. Both the silver nanoparticles and the zinc oxide nanorods were mixed in a 1:2 ratio and were further sonicated for about 1 h. Characterization was carried out in order to confirm the successful synthesis of the nanocomposite [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

“…First, 30 µL of the Ag/ZnO nanocomposite was placed on a working electrode of a paper-based sensor. The sensor was then dried using a hot plate set at approximately 60 • C. The next step involved the immobilization of the aptamer (20 µL) on the working electrode, which was adorned with the Ag/ZnO nanocomposite and dried for an hour at room temperature [36][37][38]. The detection of methamphetamine was made possible through this sensor modification.…”

Section: Immobilization Of Ag/zno Nanocomposite and The Deposition Of...mentioning

confidence: 99%

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Paper-Based Electrodes Decorated with Silver and Zinc Oxide Nanocomposite for Electro-Chemical Sensing of Methamphetamine

Anzar

Suleman

Bano

et al. 2023

Sensors

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We present the development of an electrochemical paper-based analytical device (ePAD) for the detection of methamphetamine. Methamphetamine is a stimulant that young people use as an addictive narcotic, and it must be detected quickly since it may be hazardous. The suggested ePAD has the advantages of being simple, affordable, and recyclable. This ePAD was developed by immobilizing a methamphetamine-binding aptamer onto Ag-ZnO nanocomposite electrodes. The Ag-ZnO nanocomposites were synthesized via a chemical method and were further characterized via scanning electron microscopy, Fourier transform infrared spectroscopy, and UV-vis spectrometry in terms of their size, shape, and colloidal activity. The developed sensor showed a limit of detection of about 0.1 μg/mL, with an optimum response time of about 25 s, and its extensive linear range was between 0.01 and 6 μg/mL. The application of the sensor was recognized by spiking different beverages with methamphetamine. The developed sensor has a shelf life of about 30 days. This cost-effective and portable platform might prove to be highly successful in forensic diagnostic applications and will benefit those who cannot afford expensive medical tests.

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Section: Methodsmentioning

confidence: 99%

Section: Immobilization Of Ag/zno Nanocomposite and The Deposition Of...mentioning

confidence: 99%

Paper-Based Electrodes Decorated with Silver and Zinc Oxide Nanocomposite for Electro-Chemical Sensing of Methamphetamine

Anzar

Suleman

Bano

et al. 2023

Sensors

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“…Recently, many reports have been developed on paper-based biosensors for the detection of various diseases. Sharma et al 84 developed a paper-based aptasensor for the detection of Chikungunya virus with detection limit of 1 ng/mL. In another study, Hasan et al 85 used two different setups of paper electrodes (two-to three-electrode setups) for the construction of an aptasensor to diagnose dengue virus antigen with the detection limit of 0.1 μg mL.…”

Section: Sensors and Point-of-care (Poc) Devicesmentioning

confidence: 99%

Papertronics: Marriage between Paper and Electronics Becoming a Real Scenario in Resource-Limited Settings

Hasan

Sharma

Suleman

et al. 2023

ACS Appl. Bio Mater.

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Integrating electronic applications with paper, placed next to or below printed images or graphics, can further expand the possible uses of paper substrates. Consuming paper as a substrate in the field of electronics can lead to significant innovations toward papertronics applications as paper comprises various advantages like being disposable, inexpensive, biodegradable, easy to handle, simple to use, and easily available. All of these advantages will definitely spur the advancement of the electronics field, but unfortunately, putting electronics on paper is not an easy task because, compared to plastics, the paper surface is not just rough but also porous. For example, in the case of lateral flow assay testing the sensor response is delayed if the pore size of the paper is enormous. This might be a disadvantage for most electrical devices printed directly on paper. Still, some methods make it compatible when fit with a rough, absorbent surface of the paper. Building electronic devices on a standard paper substrate have sparked much interest because of its lightweight, environmental friendliness, minimal cost, and simple fabrication. A slew of improvements have been achieved in recent years to make paper electronics perform better in various applications, including transistors, batteries, and displays. In addition, flexible electronics have gained much interest in human−machine interaction and wireless sensing. This review briefly examines the origins and fabrication of paper electronics and then moves on to applications and exciting possible paths for paper-based electronics.

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“…[9,10] Cell culture, [11,12] serological test, [13] and enzyme-linked immunosorbent assay (ELISA) [14][15][16] have been commonly applied for detecting the virus biomarker and its subsequent immunologic response of the host. Despite their well-known and established methods, the assays, as mentioned earlier, involve the untrustworthy result of detection, [17] laborious procedures with a complex, high risk of infection, expensive instruments, [18] and require highly trained health staff, [19] resulting in a relatively long detection time. To overcome this problem, developing a simple disposable point-of-care (POC) biosensor seems to be the best option for their advantages of a simple, cost-effective, and scalable platform in on-site implementation.…”

Section: Introductionmentioning

confidence: 99%

Development of disposable electrode for the detection of mosquito‐borne viruses

Nasrin

Khoris

Chowdhury

et al. 2023

Biotechnology Journal

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Development of disposable, rapid, and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen‐printed carbon electrode (SPCE) for the detection of two mosquito‐borne viruses. The biosensor fabrication has step‐wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)‐polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP‐PAni/PAni‐SPCE via the streptavidin‐biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody–antigen interaction, this disposable electrode has designed to detect mosquito‐borne infectious viruses, Chikungunya virus (CHIKV), and Zika virus (ZIKV) separately in a wide linear range of 100 fg mL−1 to 1 ng mL−1 with a low detection limit of 1.33 and 12.31 fg mL−1, respectively.

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PBIS-based system integrated with zinc–silver nanocomposite for the detection of Chikungunya virus

Cited by 4 publications

References 26 publications

Paper-Based Electrodes Decorated with Silver and Zinc Oxide Nanocomposite for Electro-Chemical Sensing of Methamphetamine

Paper-Based Electrodes Decorated with Silver and Zinc Oxide Nanocomposite for Electro-Chemical Sensing of Methamphetamine

Papertronics: Marriage between Paper and Electronics Becoming a Real Scenario in Resource-Limited Settings

Development of disposable electrode for the detection of mosquito‐borne viruses

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