pBINPLUS: An improved plant transformation vector based on pBIN19

Engelen, F.A. van; Molthoff, Jos; Conner, A. J.; Nap, Jan-Peter; Pereira, Andy; Stiekema, Willem J.

doi:10.1007/bf01969123

Cited by 473 publications

(355 citation statements)

References 6 publications

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“…The BamHI insert of EpYVV clone pGEM-EYVVAM1 isolated from Eupatorium makinoi (Saunders et al, 2003) was cloned into BamHI/BglII-digested pIC19H (Marsh et al, 1984), and the insert was subsequently cloned into this BamHI-digested intermediate to produce pIC-EYVV2AM. The tandem repeat of the EpYVV genomic DNA was excised with HindIII and cloned into pBinPlus (van Engelen et al, 1995) to produce pBin-EYVVA. The BamHI/SalI fragment of the EpYVV satellite clone pGEM-EYVVbM10 (Saunders et al, 2003) was inserted into pIC19H and the full-length NcoI insert was subsequently cloned into this intermediate to produce pIC-EYVV1.9bM.…”

Section: Methodsmentioning

confidence: 99%

Replication promiscuity of DNA-β satellites associated with monopartite begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA-β satellite localizes sequences involved in replication

Saunders

Briddon

Stanley

2008

Journal of General Virology

102

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Pseudorecombination studies in Nicotiana benthamiana demonstrate that Ageratum yellow vein virus (AYVV) and Eupatorium yellow vein virus (EpYVV) can functionally interact with DNA-β satellites associated with AYVV, EpYVV, cotton leaf curl Multan virus (CLCuMV) and honeysuckle yellow vein virus (HYVV). In contrast, CLCuMV shows some specificity in its ability to interact with distinct satellites and HYVV is able to interact only with its own satellite. Using an N. benthamiana leaf disk assay, we have demonstrated that HYVV is unable to trans-replicate other satellites. To investigate the basis of trans-replication compatibility, deletion mutagenesis of AYVV DNA-β has been used to localize the origin of replication to approximately 360 nt, encompassing the ubiquitous nonanucleotide/stem–loop structure, satellite conserved region (SCR) and part of the intergenic region immediately upstream of the SCR. Additional deletions within this intergenic region have identified a region that is essential for replication. The capacity for DNA-β satellites to functionally interact with distinct geminivirus species and its implications for disease diversification are discussed.

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Section: Methodsmentioning

confidence: 99%

Replication promiscuity of DNA-β satellites associated with monopartite begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA-β satellite localizes sequences involved in replication

Saunders

Briddon

Stanley

2008

Journal of General Virology

102

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“…In total six different combinations of promoter and terminator sequences were constructed (p1.2MdRbcS-gus-tMdRbcS; p1.2MdRbcS-gus-tnos; p1.6MdRbcS-gus-tMdRbcS; p1.6MdRbcS-gus-tnos; pCaMV35S-gus-tMdRbcS, and CaMV35S-gus-tnos (see Fig. 1)), and these were introduced as AscI-PacI fragments in the multiple cloning site of the binary vector pBINplus (van Engelen et al 1995). The ultimate constructs were transferred to the supervirulent A. tumefaciens strain Agl0 (Lazo et al 1991).…”

Section: Construction Of Transformation Vectorsmentioning

confidence: 99%

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

Schaart

Tinnenbroek-Capel

Krens

2010

Tree Genetics & Genomes

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For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically.

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“…To generate transgenic plants expressing the AtTHIC promoter-GUS, a 1.5-kb genomic fragment upstream of the AtTHIC transcription start condon was amplified by PCR using the primers 5′-GGT ACC CAC ACA CGA AAT GAT CAA AG-3′ and 5′-CCA TGG AGC TGG AGA CAA ACG AAA AT-3′, and cloned at the NcoI/KpnI sites of pJIT166 (http://www.pgreen.ac.uk) to generate the AtTHIC promoter-GUS plasmid. Then the expression cassette of the AtTHIC promoter -GUS was cut out from pJIT166 by XhoI/KpnI and cloned into pBINPLUS [43]. The constructs were introduced into the GV3101 strain of A. tumefaciens for Arabidopsis transformation according to the floral dip protocol [44].…”

Section: Plasmid Construction and Transformationmentioning

confidence: 99%

AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

Kong

Zhu

et al. 2008

Cell Res

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Thiamine (vitamin B1) is an essential compound for organisms. It contains a pyrimidine ring structure and a thiazole ring structure. These two moieties of thiamine are synthesized independently and then coupled together. Here we report the molecular characterization of AtTHIC, which is involved in thiamine biosynthesis in Arabidopsis. AtTHIC is similar to Escherichia coli ThiC, which is involved in pyrimidine biosynthesis in prokaryotes. Heterologous expression of AtTHIC could functionally complement the thiC knock-out mutant of E. coli. Downregulation of AtTHIC expression by T-DNA insertion at its promoter region resulted in a drastic reduction of thiamine content in plants and the knock-down mutant thic1 showed albino (white leaves) and lethal phenotypes under the normal culture conditions. The thic1 mutant could be rescued by supplementation of thiamine and its defect functions could be complemented by expression of AtTHIC cDNA. Transient expression analysis revealed that the AtTHIC protein targets plastids and chloroplasts. AtTHIC was strongly expressed in leaves, flowers and siliques and the transcription of AtTHIC was downregulated by extrinsic thiamine. In conclusion, AtTHIC is a gene involved in pyrimidine synthesis in the thiamine biosynthesis pathway of Arabidopsis, and our results provide some new clues for elucidating the pathway of thiamine biosynthesis in plants.

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pBINPLUS: An improved plant transformation vector based on pBIN19

Cited by 473 publications

References 6 publications

Replication promiscuity of DNA-β satellites associated with monopartite begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA-β satellite localizes sequences involved in replication

Replication promiscuity of DNA-β satellites associated with monopartite begomoviruses; deletion mutagenesis of the Ageratum yellow vein virus DNA-β satellite localizes sequences involved in replication

Isolation and characterization of strong gene regulatory sequences from apple, Malus × domestica

AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana

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