PbCOP1.1 Contributes to the Negative Regulation of Anthocyanin Biosynthesis in Pear

Wu, Meng; Su, Min; Li, Xieyu; Song, Linyan; Liu, Jianlong; Zhai, Rui; Cong, Liu; Yue, Rongrong; Yang, Chengquan; Ma, Fengwang; Xu, Lingfei; Wang, Zhigang

doi:10.3390/plants8020039

Cited by 27 publications

(22 citation statements)

References 47 publications

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“…It has been reported that PIF3 degrades rapidly after irradiation of dark-grown seedlings in a process controlled by phytochromes (Bauer et al, 2004). Transgenic pears overexpressing of PbCOP1.1 showed decreased expression levels of anthocyanin-related synthetic genes and negatively regulated the coloration of fruits (Wu et al, 2019). Through ubiquitination, COP1 degrades HY5, a promoter of anthocyanin biosynthesis, and thereby, reduces the accumulation of anthocyanin in apple (An et al, 2017).…”

Section: Sunlight Stimulates Coloration Of Apple Fruit By Down-regulating the Expression Of Light-responsive Genesmentioning

confidence: 99%

“…PIFs were found to interact with phytochromes and participate in anthocyanin biosynthesis by binding to the promoters of anthocyanin biosynthesis-related genes (Liu et al, 2015;Shin et al, 2007). The functional of COP1 on the expression of anthocyanin biosynthetic genes was recently studied in Arabidopsis (Maier et al, 2013), pear (Wu et al, 2019), and apple (An et al, 2017;Li et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

Giap

Kim

Lee

et al. 2021

International Journal of Fruit Science

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Anthocyanin accumulation is responsible for the red color of the skin of apple fruits (Malus × domestica Borkh.). Environmental stimuli, such as light, temperature, soil factors, tree factors, and the application of chemicals can alter the synthesis of anthocyanins. Herein, we examined the expression of genes related to anthocyanin biosynthesis in the fruit of apple cultivars, 'Summer Prince' and 'Arisoo' at the early stage of fruit development 60 DAFB (days after full bloom). The expression of genes, which included structural and regulatory genes involved in anthocyanin biosynthesis, and light-responsive genes was determined in fruits developed under the effect of sunlight exposure for seven days. Apple fruits were divided in to: 'reflected sunlight', 'bagged', and 'control' groups. The expression levels of the anthocyanin synthesis-related genes were significantly different among the groups and between the shaded and reflected sides. We also determined the difference in coloration patterns in the different groups using the colorimetric coordinates method. The shaded side of apple fruits quickly turned more redder after exposure to reflected sunlight and the coloration was almost similar to that on the exposed side after the treatment. Strong correlation coefficients between anthocyanin-related gene expression levels and coloration patterns were obtained. This study shows that sunlight plays an important role in stimulating the coloration of apple fruit by regulating the expression of genes involved in anthocyanin accumulation during the early stage of fruit development. The present study assumes importance because redder fruits, generated through a non-transgenic approach would be more acceptable to consumers.

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Section: Sunlight Stimulates Coloration Of Apple Fruit By Down-regulating the Expression Of Light-responsive Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

Giap

Kim

Lee

et al. 2021

International Journal of Fruit Science

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“…The transient overexpression method referred to previous studies [16,17]. The complete coding sequences (CDS) of PbLAC4-like and PbMYB26 were ampli ed by PCR from 'Red Zaosu' cDNA and then fused into the multiple cloning site (MCS) of pGreen 0029-62SK vector respectively to form 62SK-PbLAC4-like and 62SK-PbMYB26 plasmids.…”

Section: Transient Overexpression Assay In Pear Fruitlet Peelmentioning

confidence: 99%

“…MYB10, MYB10b, ERF22, REVEILLE promoted the biosynthesis of anthocyanin by activating the expression of anthocyanin biosynthesis genes in pear [14][15][16]. COP1.1 and MYB120 were identi ed as inhibitory regulators of anthocyanin biosynthesis in pear [17,18]. bHLH3, MYB88, MYB124, NAC52 and in apple [19][20][21], MYB75, MYB90, MYB113 and MYB114 in Arabidopsis thaliana [22][23][24] also were shown to promote anthocyanin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

PbLAC4-like, activated by PbMYB26, related to the degradation of anthocyanin during color fading in pear

Zhao

Xiang

Zhang

et al. 2021

Preprint

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Background Anthocyanin degradation results in the loss of red color in leaves, petals and receptacles during development. But the degradation mechanism is not fully investigated. It is vital to study the degradation mechanism of anthocyanin in pear for promoting the accumulation of anthocyanin and inhibiting the red fading in pear. Results Here, we reported that laccase encoded by PbLAC4-like was associated with anthocyanin degradation in pear. The expression pattern of PbLAC4-like was negatively correlated with the content of anthocyanin during the color fading process of pear leaves, petals and receptacles. Phylogenetic analysis and sequence alignment revealed that PbLAC4-like played a vital role in anthocyanin degradation. Thus, the degradation of anthocyanin induced by PbLAC4-like was further verified by transient assays and prokaryotic expression. More than 80% of anthocyanin compounds were degraded by transiently over-expressed PbLAC4-like in pear fruitlet peel. The activity of crude enzyme to degrade anthocyanin in leaves at different stages was basically consistent with the expression of PbLAC4-like. The anthocyanin degradation ability of prokaryotic induced PbLAC4-like protein was also verified by enzyme activity assay. Besides, we also identified PbMYB26 as a positive regulator of PbLAC4-like. Yeast one-hybrid and dual luciferase assay results showed that PbMYB26 activated PbLAC4-like expression by directly binding to the PbLAC4-like promoter. Conclusions Taken together, the PbLAC4-like activated by PbMYB26, was involved in the degradation of anthocyanin, resulting in the redness fading in different pear tissues.

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“…Quercetin and kaempferol derivates accumulate in Arabidopsis seedlings ( Stracke et al, 2010b ) acting both as UV protectants and free-radical scavengers ( Agati and Tattini, 2010 ). UV-B mediated flavonoid accumulation is UVR8 dependent ( Demkura and Ballare, 2012 ; Morales et al, 2013 ), leading to an induction of CHS, chalcone flavanone isomerase (CHI), flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) ( Hideg et al, 2013 ), all of which are HY5 inducible ( Shin et al, 2013 ; Wu et al, 2019 ), Interestingly dihydroflavonol reductase (DFR) was only shown to be induced in UV-A conditions ( Morales et al, 2013 ). However, the role of UVR8 in the induction of DFR transcript is not COP1 regulated under UV-B conditions ( Oravecz et al, 2006 ) and therefore hints at an interaction between photoreceptors ( Morales et al, 2013 ) below 350 nm ( Morales et al, 2013 ; Rai et al, 2020 ).…”

Section: Uv Regulated Specialized Metabolites and Hormones Leading Tmentioning

confidence: 99%

Ultraviolet Radiation From a Plant Perspective: The Plant-Microorganism Context

et al. 2020

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Ultraviolet (UV) radiation directly affects plants and microorganisms, but also alters the species-specific interactions between them. The distinct bands of UV radiation, UV-A, UV-B, and UV-C have different effects on plants and their associated microorganisms. While UV-A and UV-B mainly affect morphogenesis and phototropism, UV-B and UV-C strongly trigger secondary metabolite production. Short wave (<350 nm) UV radiation negatively affects plant pathogens in direct and indirect ways. Direct effects can be ascribed to DNA damage, protein polymerization, enzyme inactivation and increased cell membrane permeability. UV-C is the most energetic radiation and is thus more effective at lower doses to kill microorganisms, but by consequence also often causes plant damage. Indirect effects can be ascribed to UV-B specific pathways such as the UVR8-dependent upregulated defense responses in plants, UV-B and UV-C upregulated ROS accumulation, and secondary metabolite production such as phenolic compounds. In this review, we summarize the physiological and molecular effects of UV radiation on plants, microorganisms and their interactions. Considerations for the use of UV radiation to control microorganisms, pathogenic as well as non-pathogenic, are listed. Effects can be indirect by increasing specialized metabolites with plant pre-treatment, or by directly affecting microorganisms.

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PbCOP1.1 Contributes to the Negative Regulation of Anthocyanin Biosynthesis in Pear

Cited by 27 publications

References 47 publications

Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

PbLAC4-like, activated by PbMYB26, related to the degradation of anthocyanin during color fading in pear

Ultraviolet Radiation From a Plant Perspective: The Plant-Microorganism Context

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