PBC Screen: An IgG/IgA dual isotype ELISA detecting multiple mitochondrial and nuclear autoantibodies specific for primary biliary cirrhosis

Liu, Haiying; Norman, Gary L.; Shums, Zakera; Worman, Howard J.; Krawitt, Edward L.; Bizzaro, Nicola; Vergani, Diego; Bogdanos, Dimitrios P.; Dalekos, George Ν.; Milkiewicz, Piotr; Czaja, Albert J.; Heathcote, E. Jenny; Hirschfield, Gideon M.; Tan, Eng M.; Miyachi, Kiyomitsu; Bignotto, Monica; Battezzati, Pier Maria; Lleo, Ana; Leung, Patrick S.C.; Podda, Mauro; Gershwin, M. Eric; Invernizzi, Pietro

doi:10.1016/j.jaut.2010.09.005

Cited by 128 publications

(70 citation statements)

References 41 publications

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“…The antibody has also been detected in patients with AMA -negative PBC and thus is considered a useful marker for serological diagnosis of PBC 40) . The anti -PML antibody has been detected in PBC patients positive for anti -Sp100 antibody, and patients positive for these antibodies have been shown to show faster progression of PBC than those negative for these antibodies 41,42) .…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies by Line Immunoassay in Patients With Primary Biliary Cirrhosis

Saito¹,

Takahashi²,

Abe³

et al. 2012

Fukushima J. Med. Sci.

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: Objectives : We attempted to measure multiple autoantibodies simultaneously using line immunoassay (LIA) in patients with primary biliary cirrhosis (PBC) with or without anti -mitochondrial antibody (AMA) and patients with PBC -autoimmune hepatitis (AIH) overlap, and we examined the clinical significance of measuring these autoantibodies. Methods : The study population consisted of 80 patients with PBC (including 12 AMA -negative patients), 16 patients with PBC -AIH overlap and 40 patients with AIH as controls. Nine antibodies (AMA -M2, M2 -3E, Sp100, PML, gp210, Ro -52, LKM -1, LC -1 and SLA/LP) were detected by LIA, and AMA -M2 and anti -centromere antibody (ACA) were detected by ELISA. We examined the relationship between these autoantibodies and clinical findings. Results : The positive prevalence of each autoantibody and ACA in the PBC group, as determined by LIA, was as follows : 13.8% for anti -Sp100, 8.7% for anti -PML, 40% for anti -gp210 and 27.5% for anti -Ro -52 antibodies and 32.5% for ACA. In the PBC -AIH overlap group, the prevalence of anti -gp210 antibody (68.7%) and that of anti -Ro -52 antibody (81.2%) were significantly higher than those in the PBC and AIH groups. Only a few patients were positive for 2 or more autoantibodies. Nine patients were determined to be negative for all autoantibodies by LIA, of whom 7 were positive for ACA. Patients positive for anti -gp210 antibody included more patients classified as stage 4 on histology than did the negative group. Those positive for ACA included more patents with varices than did the negative group. Conclusion : LIA can measure multiple autoantibodies simultaneously and thus is considered useful in diagnosing PBC and PBC -AIH overlap. In addition, ACA is a useful marker for identifying AMA -negative PBC.

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Section: Discussionmentioning

confidence: 99%

Autoantibodies by Line Immunoassay in Patients With Primary Biliary Cirrhosis

Saito¹,

Takahashi²,

Abe³

et al. 2012

Fukushima J. Med. Sci.

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“…Early studies reported positive MIT3 AMA results in approximately 90% of PBC sera that tested negative for AMA by conventional immunofluorescence techniques [16]. These findings have been confirmed by subsequent studies in large cohorts and from various groups [17,18]. These findings put in question what has been described as AMA-negative PBC and strongly indicate that the vast majority of biopsy-proven PBC contain AMA if reliable, highly sensitive and specific tests are used for their detection.…”

Section: What Is New In Pbc: Serological Diagnosis – Are There Any ‘Tmentioning

confidence: 84%

“…microtiter plate dot blot or line blots, and even fluorescent bead-based assays, have been developed with up to 97% sensitivity at 98% specificity for PBC [17,18,19]. These methods are now widely available as commercial kits and are based on preparations of PDC from porcine or bovine heart mixed with recombinantly produced human E2 subunits, designer antigens composed of all three E2 subunits, or a mixture of native and designer antigens.…”

Section: What Is New In Pbc: Serological Diagnosis – Are There Any ‘Tmentioning

confidence: 99%

What Is New in Primary Biliary Cirrhosis?

Bogdanos

Gershwin²

2012

Dig Dis

Self Cite

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Primary biliary cirrhosis is a chronic cholestatic liver disease characterized by immune-mediated destruction of the small- and medium-sized intrahepatic bile ducts. The disease mainly affects middle-aged women who frequently suffer from concomitant autoimmune diseases. This review discusses the most recent findings in the epidemiology, natural history and approaches used for the accurate diagnosis of the disease. We critically review emerging data reporting on the role of genetics such as those of genome-wide association studies. Special attention is given to recent studies reporting on the immunopathogenesis of the disease and the role of plasma cells, apotopes, immunosenescence, autophagy and the involvement of components of the innate immunity in the induction of this enigmatic disease.

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“…An enormous effort has been made to define the genetics and immuobiology of human autoimmue liver diseases in both human and mouse models [59][60][61][62][63][64][65][66][67][68][69][70][71][72]. Yet there remains an enormous gap between these investigative efforts and clinical translation.…”

Section: Discussionmentioning

confidence: 99%