PbbHLH4 regulates floral monoterpene biosynthesis in Phalaenopsis orchids

Chuang, Yu Chen; Hung, Yi Chu; Tsai, Wen Chieh; Chen, Wen Huei; Chen, Hong Hwa

doi:10.1093/jxb/ery246

Cited by 74 publications

(63 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The functions of these regulators with PeMYB11 for anthocyanin accumulation and pigmentation patterning were further investigated by a transient overexpression approach in Phalaenopsis orchids. 2015; Chuang et al, 2018). Agrobacterium tumefaciens containing various regulatory genes were infiltrated into white petals of Phalaenopsis Sogo Yukidian 'V39 alone or with various bHLH transcription factors and/or MYB27-and MYBx-like repressors.…”

Section: Other Regulatory Factors Related To Harlequin Flower Formatimentioning

confidence: 99%

A HORT1 Retrotransposon Insertion in the PeMYB11 Promoter Causes Harlequin/Black Flowers in Phalaenopsis Orchids

Hsu

Jeng

et al. 2019

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

The harlequin/black flowers in Phalaenopsis orchids contain dark purple spots and various pigmentation patterns, which appeared as a new color in 1996. We analyzed this phenotype by microscopy, HPLC, gene functional characterization, genome structure analysis, and transient overexpression system to obtain a better understanding of the black color formation in Phalaenopsis orchids. Most mesophyll cells of harlequin flowers showed extremely high accumulation of anthocyanins as well as a high expression of Phalaenopsis equestris MYB11 (PeMYB11) as the major regulatory R2R3-MYB transcription factor for regulating the production of the black color. In addition, we analyzed the expression of basic helix-loop-helix factors, WD40 repeat proteins, and MYB27-and MYBx-like repressors for their association with the spot pattern formation. To understand the high expression of PeMYB11 in harlequin flowers, we isolated the promoter sequences of PeMYB11 from red and harlequin flowers. A retrotransposon, named Harlequin Orchid RetroTransposon 1 (HORT1), was identified and inserted in the upstream regulatory region of PeMYB11. The insertion resulted in strong expression of PeMYB11 and thus extremely high accumulation of anthocyanins in the harlequin flowers of the Phalaenopsis Yushan Little Pearl variety. A dual luciferase assay showed that the insertion of HORT1 enhanced PeMYB11 expression by at least 2-fold compared with plants not carrying the insertion. Furthermore, the presence of HORT1 explains the high mutation rates resulting in many variations of pigmentation patterning in harlequin flowers of Phalaenopsis orchids.

show abstract

Section: Other Regulatory Factors Related To Harlequin Flower Formatimentioning

confidence: 99%

A HORT1 Retrotransposon Insertion in the PeMYB11 Promoter Causes Harlequin/Black Flowers in Phalaenopsis Orchids

Hsu

Jeng

et al. 2019

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In orchids, PbGDPS , which encodes GPP synthase, may play a key role in regulating the biosynthesis of monoterpenes (geraniol and linalool) in P. bellina [ 14 ]. In addition, the transcript levels of two TPS genes ( PbTPS5 and PbTPS10 ) are consistent with the production of geraniol and linalool in P. bellina [ 15 ], although their functionality has not yet been verified. Although geraniol is an important floral volatile compound in D. officinale , a medicinal orchid [ 7 ], the GES gene responsible for geraniol biosynthesis in D. officinale has not yet been characterized.…”

Section: Introductionmentioning

confidence: 76%

“…From D. officinale genomic annotation data, three candidate GES sequences with best matches to known GES proteins [ 6 , 12 , 13 , 15 ] were retrieved by BLASTN, and named DoGES1, DoGES2, and DoGES3 ( Table S1 ). Multiple sequence alignment demonstrated that three DoGES proteins had highly conserved aspartate-rich motifs (DDxxD) and NSE/DTE motifs at the C -terminal, and an RRX 8 W domain at the N -terminal ( Figure 2 ), suggesting that DoGES1-3 were all TPSs.…”

Section: Resultsmentioning

confidence: 99%

“…The GES gene, which was first cloned and functionally identified in sweet basil ( O. basilicum ), was shown to have the function of geraniol catalysis [ 6 ]. Subsequently, GES has been fairly extensively studied in many plant species, such as C. sinensis [ 12 ], L. odoratus [ 13 ], Gardenia jasminoides [ 30 ], N. tabacum [ 31 ], and P. bellina [ 15 ]. The second pathway for the production of geraniol is a phosphatase-based non-canonical pathway for monoterpene biosynthesis that involves NUDX [ 9 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Zhao

Silva³

et al. 2020

IJMS

View full text Add to dashboard Cite

Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids.

show abstract

“…De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for 11 diverse P. equestris tissue performed by Niu et al (2016), and obtained transcriptomes from root, stem, seed and floral organs, and found 24, 21, 22 and 7 disease resistance (R) genes in the flower bud, root, stem and in the 7-day-seeds, respectively and did not observe the YABBY gene family (use in determining leaf polarity) in roots and seeds. Eight transcripts have been identified during the comparative transcriptome analysis between scented and scentless orchids, among them PbbHLH4 regulates floral monoterpene biosynthesis in Phalaenopsis orchid (Chuang et al 2018). Phalaenopsis flowering locus VE (PaFVE) gene has been characterized by Koh et al (2018) via spatial and temporal expression studies.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Balilashaki

Zakizadeh

Olfati

et al. 2019

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

With recent advances in high-throughput sequencing (HTS) technologies to improve plants, there is a need to release orchid specific genomic resources and platforms that are crucial for managing omics elements in systematic manner. Authors provide details about the recent developments in biotechnological techniques, genomics, transcriptomics, proteomics, metabolomics and their applications for the industrial production, propagation, conservation and manipulation of Phalaenopsis orchid. Plant Tissue Cult. & Biotech. 29(1): 133-149, 2019 (June)

show abstract

PbbHLH4 regulates floral monoterpene biosynthesis in Phalaenopsis orchids

Abstract: A basic helix-loop-helix transcription factor PbbHLH4 induces the biosynthesis of monoterpenes for floral scent in Phalaenopsis orchids.

Cited by 74 publications

References 75 publications

A HORT1 Retrotransposon Insertion in the PeMYB11 Promoter Causes Harlequin/Black Flowers in Phalaenopsis Orchids

A HORT1 Retrotransposon Insertion in the PeMYB11 Promoter Causes Harlequin/Black Flowers in Phalaenopsis Orchids

Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Contact Info

Product

Resources

About