“…For HPV ISH, specimens were processed as previously described using the Leica Bond III autostainer (Leica Biosystem, Buffalo Grove, IL). 25 The RNA ISH used RNAscope probes as follows: high-risk cocktail including HPV types 16,18,26,31,33,35,39,45,51,52,53,56,58,59, 66, 68, 73, 82 (HPV-HR18, Cat# 312598; Advanced Cell Diagnostics, Hayward, CA), HPV-16 (HPV-16, Cat# 311528), HPV-18 (HPV-18, Cat# 311538), low-risk cocktail including HPV types 6 and 11 (HPV6/11, Cat# 415218). RNA ISH was considered positive if punctate brown signals were present in the cytoplasm and/or nucleus.…”