Pax5 Maintains Cellular Identity by Repressing Gene Expression Throughout B Cell Differentiation

Carotta, Sebastian; Holmes, Melissa L.; Pridans, Clare; Nutt, Stephen L.

doi:10.4161/cc.5.21.3396

Cited by 21 publications

(15 citation statements)

References 50 publications

(61 reference statements)

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“…Active and recrudescent testes are actively engaged in transcription and genes crucial in initiation and regulation of transcription were Pax5 [64]–[66], Dhx32 [67], Meis1 [68] and Nfi [69], [70] are up-regulated. These genes play critical role in initiation and maintenance of active transcriptional machinery in the cell.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Spermatogenically Regressed, Recrudescent and Active Phase Testis of Seasonally Breeding Wall Lizards Hemidactylus flaviviridis

et al. 2013

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BackgroundReptiles are phylogenically important group of organisms as mammals have evolved from them. Wall lizard testis exhibits clearly distinct morphology during various phases of a reproductive cycle making them an interesting model to study regulation of spermatogenesis. Studies on reptile spermatogenesis are negligible hence this study will prove to be an important resource.Methodology/Principal FindingsHistological analyses show complete regression of seminiferous tubules during regressed phase with retracted Sertoli cells and spermatognia. In the recrudescent phase, regressed testis regain cellular activity showing presence of normal Sertoli cells and developing germ cells. In the active phase, testis reaches up to its maximum size with enlarged seminiferous tubules and presence of sperm in seminiferous lumen. Total RNA extracted from whole testis of regressed, recrudescent and active phase of wall lizard was hybridized on Mouse Whole Genome 8×60 K format gene chip. Microarray data from regressed phase was deemed as control group. Microarray data were validated by assessing the expression of some selected genes using Quantitative Real-Time PCR. The genes prominently expressed in recrudescent and active phase testis are cytoskeleton organization GO 0005856, cell growth GO 0045927, GTpase regulator activity GO: 0030695, transcription GO: 0006352, apoptosis GO: 0006915 and many other biological processes. The genes showing higher expression in regressed phase belonged to functional categories such as negative regulation of macromolecule metabolic process GO: 0010605, negative regulation of gene expression GO: 0010629 and maintenance of stem cell niche GO: 0045165.Conclusion/SignificanceThis is the first exploratory study profiling transcriptome of three drastically different conditions of any reptilian testis. The genes expressed in the testis during regressed, recrudescent and active phase of reproductive cycle are in concordance with the testis morphology during these phases. This study will pave the way for deeper insight into regulation and evolution of gene regulatory mechanisms in spermatogenesis.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Spermatogenically Regressed, Recrudescent and Active Phase Testis of Seasonally Breeding Wall Lizards Hemidactylus flaviviridis

et al. 2013

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“…For example, the formation of B lymphocytes depends upon the activation of expression of the transcription factor Pax 5 [81]. Uncommitted hematopoietic progenitors display a low-level expression of genes associated with multiple mutually exclusive lineages [82], and this promiscuity needs to be restricted for the differentiation of B lymphocyte-specific cell lineage identity to occur during lymphopoiesis.…”

Section: Mechanisms Of Inappropriate Gene Expression and Silencingmentioning

confidence: 99%

Inappropriate gene expression in human cancer and its far-reaching biological and clinical significance

Tarin

2011

Cancer Metastasis Rev

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This article provides a broad overview of the field of inappropriate gene expression in many organisms across the animal and plant kingdoms as well as its importance to human disease in general and cancer in particular. Study of the topic is especially important for understanding how the chaotic maelstrom of evolving and cascading regulatory genetic interactions in an advancing cancer produces its clinical effects and for designing pragmatic solutions to how such disorder might eventually be tamed. It is emphasized that the topic warrants much more attention in research and in clinical practice because of the added value it brings to refining cancer diagnosis and treatment and to the assessment of prognostic markers. It is also particularly relevant to understanding the etiology and extensive clinical manifestations of paraneoplastic syndromes affecting multiple organs remote from the tumor and the treatment or amelioration of the substantial morbidity that they cause in cancer patients. More broadly, inappropriate expression can be caused by a number of mechanisms including mutations, rearrangements of the genome, and viral insertions and, under prolonged selection pressures, also has the potential to be an agent of evolutionary change.

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“…Pax5, a transcription factor critically involved in the specification of the B cell lineage, normally represses the expression of these genes in resting B cells. 4 However, while loss of Pax5 expression is necessary to promote ASC formation, 5,6 whether the loss of Pax5 is sufficient to promote ASC formation remains controversial. 6,7 Along these lines, another critical target of BLIMP1-mediated repression is BCL6.…”

Section: Differentiation Through Repressionmentioning

confidence: 99%

Phosphatidylinositol 3-kinase (PI3K) Signaling and Regulation of the Antibody Response

Omori

Rickert²

2007

Cell Cycle

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Pax5 Maintains Cellular Identity by Repressing Gene Expression Throughout B Cell Differentiation

Cited by 21 publications

References 50 publications

Transcriptome Analysis of Spermatogenically Regressed, Recrudescent and Active Phase Testis of Seasonally Breeding Wall Lizards Hemidactylus flaviviridis

Transcriptome Analysis of Spermatogenically Regressed, Recrudescent and Active Phase Testis of Seasonally Breeding Wall Lizards Hemidactylus flaviviridis

Inappropriate gene expression in human cancer and its far-reaching biological and clinical significance

Phosphatidylinositol 3-kinase (PI3K) Signaling and Regulation of the Antibody Response

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