Pax3 overexpression induces cell aggregation and perturbs commissural axon projection during embryonic spinal cord development

Lin, Juntang; Fu, Shishun; Yang, Ciqing; Redies, Christoph

doi:10.1002/cne.24146

Cited by 8 publications

(3 citation statements)

References 57 publications

(71 reference statements)

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“…Venters et al (2004) confirmed that the Pax3 antibody stains an single band of about 60 kDa on Western blots of extract from E3 chicken neural tube and notochord. In addition, the expression pattern of Pax3 protein obtained in the present study was similar to that reported previously for Pax3 protein (Lin et al, 2017).…”

Section: Methodssupporting

confidence: 91%

“…In this study, we also showed that the overexpression of Shh significantly inhibited the commissural axons from projecting to the contralateral side. Our previous study indicated that the transcription factors Pax3 play important roles in induces cell aggregation and perturbs commissural axon projection during embryonic spinal cord development (Lin et al, 2017). In this study showed that the shh overexpression inhibited the expression of Pax3 and Pax7.…”

Section: Discussionmentioning

confidence: 99%

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The effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryo development

Yang¹,

et al. 2017

Preprint

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Sonic hedgehog (Shh) is a vertebrate homologue of the secreted Drosophila protein 1 9 hedgehog, and is expressed by the notochord and the floor plate in the developing 2 0 spinal cord. Shh provides signals relevant for positional information, cell proliferation, 2 1 and possibly cell survival depending on the time and location of the expression. 2 2Although the role of Shh in providing positional information in the neural tube has 2 3 been experimentally proven, the exact underlying mechanism still remains unclear. In 2 4this study, we report that overexpression of Shh affects motor neuron positioning in 2 5 the spinal cord during chicken embryo development by inducing abnormalities in the 2 6 structure of the motor column and motor neuron integration. In addition, Shh 2 7 overexpression inhibits the expression of dorsal transcription factors and commissural 2 8 axon projections. Our results indicate that correct location of Shh expression is the 2 9 key to the formation of the motor column. In conclusion, the overexpression of Shh in 3 0 the spinal cord not only affects the positioning of motor neurons, but also induces 3 1 abnormalities in the structure of the motor column.

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Section: Methodssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryo development

Yang¹,

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our previous study indicated that the transcription factor Pax3 play important roles in inducing cell aggregation and perturbing commissural axon projection during embryonic spinal cord development. 45…”

Section: Discussionmentioning

confidence: 99%

Effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryonic development

Yang

et al. 2019

J Cellular Molecular Medi

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Sonic hedgehog (SHH) is a vertebrate homologue of the secreted Drosophila protein hedgehog and is expressed by the notochord and floor plate in the developing spinal cord. Sonic hedgehog provides signals relevant for positional information, cell proliferation and possibly cell survival, depending on the time and location of expression. Although the role of SHH in providing positional information in the neural tube has been experimentally proven, the underlying mechanism remains unclear. In this study, in ovo electroporation was employed in the chicken spinal cord during chicken embryo development. Electroporation was conducted at stage 17 (E2.5), after electroporation the embryos were continued incubating to stage 28 (E6) for sampling, tissue fixation with 4% paraformaldehyde and frozen sectioning. Sonic hedgehog and related protein expressions were detected by in situ hybridization and fluorescence immunohistochemistry and the results were analysed after microphotography. Our results indicate that the ectopic expression of SHH leads to ventralization in the spinal cord during chicken embryonic development by inducing abnormalities in the structure of the motor column and motor neuron integration. In addition, ectopic SHH expression inhibits the expression of dorsal transcription factors and commissural axon projections. The correct location of SHH expression is vital to the formation of the motor column. Ectopic expression of SHH in the spinal cord not only affects the positioning of motor neurons, but also induces abnormalities in the structure of the motor column. It leads to ventralization in the spinal cord, resulting in the formation of more ventral neurons forming during neuronal formation.

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Wnt3a Ectopic Expression Interferes Axonal Projection and Motor Neuron Positioning During the Chicken Spinal Cord Development

Yang

Zhang

et al. 2018

J Mol Neurosci

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The formation of dorsal-ventral axis of the spinal cord is controlled largely by dorsal signals such as Wnts (which are members of the wingless + MMTV integrants, Int family), besides ventral signals such as sonic hedgehog (Shh). Wnt3a, one of the Wnt family members, is involved in multiple cellular functions, including self-renewal, proliferation, differentiation, and motility. Here, we aim to study the mechanism of the regulation of chicken spinal cord patterning by Wnt3a. In this study, Wnt3a was ectopically expressed in the spinal cord of developing chicken embryos by in ovo electroporation. The results of immunofluorescent staining revealed that Wnt3a ectopic expression caused the abnormality of commissural axonal projection and the formation of nerve fibers was interrupted. It is worth noting that neurons in the ventricular zone, especially motor neurons, could not migrate laterally after the Wnt3a overexpression, which led to the malformation of motor column. In addition, we found that neurons could not protrude axons outwardly after overexpression of Wnt3a in the spinal cord. It was also found that Wnt3a overexpression inhibited the outgrowth of processes in culturing SH-SY5Y cells. In conclusion, we proposed that Wnt3a regulates neuronal morphology, which subsequently disrupts axonal projection and motor neuron positioning during spinal cord development.

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Pax3 overexpression induces cell aggregation and perturbs commissural axon projection during embryonic spinal cord development

Cited by 8 publications

References 57 publications

The effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryo development

The effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryo development

Effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryonic development

Wnt3a Ectopic Expression Interferes Axonal Projection and Motor Neuron Positioning During the Chicken Spinal Cord Development

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