Pax-QNR/Pax-6, a paired box- and homeobox-containing gene expressed in neurons, is also expressed in pancreatic endocrine cells.

Turque, Nathalie; Plaza, Serge; Radvanyi, François; Carrière, Catherine; Saule, Simon

doi:10.1210/mend.8.7.7984154

Cited by 53 publications

(54 citation statements)

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“…The large overlap between neurons and pancreatic endocrine cells in the molecular program of differentiation (Ahlgren et al, 1997;Alpert et al, 1988;Gradwohl et al, 2000;Naya et al, 1997; Sosa-Pineda et al, 1997; Sussel et al, 1998;Turque et al, 1994) and the knowledge that nestin is a marker of neuronal stem cells (Cattaneo and McKay, 1990;Johansson et al, 1999;Lendahl et al, 1990) drove the search in the pancreas for a nestin-positive population that would generate the cells of the endocrine and exocrine lineages. Previous reports indicated that nestin was expressed by mesenchymal cells of pancreas during development (Selander and Edlund, 2002), by the epithelium of the pancreatic primordium, and by acinar but not endocrine cells produced by this embryonic layer (Delacour et al, 2004;Esni et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The fact that islet cells and neurons have similar molecular phenotypes (Ahlgren et al, 1997;Alpert et al, 1988;Gradwohl et al, 2000;Naya et al, 1997;Sander et al, 2000;Sosa-Pineda et al, 1997;Sussel et al, 1998;Turque et al, 1994) raised the possibility that precursor cells of islets, like those of brain, express the intermediate filament nestin (Cattaneo and McKay, 1990;Lendahl et al, 1990), a classical marker of neuronal stem cells. This possibility was supported by the discovery of nestin ϩ cells in epithelial cells of the pancreatic bud of mouse embryos from e-10 to e-15 (Esni et al, 2004), raising the prospect that these cells were endocrine precursor cells.…”

Section: Introductionmentioning

confidence: 99%

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Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Kedees¹,

Guz²,

Vuguin³

et al. 2007

Developmental Dynamics

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Nestin, a marker of neural stem cells, is also expressed by cells located in the epithelium of the pancreatic primordium and by a subpopulation of exocrine cells but not by endocrine cells. These findings raised the possibility that the pancreatic epithelium is heterogeneous and comprised of subpopulations of exocrine/ nestin-positive and endocrine/nestin-negative precursor cells. We examined this issue in two mutant mouse models characterized by protracted expression of several embryonal properties in islet cells. One mutant line comprises mice lacking mature glucagon due to abrogation of proprotein convertase-2 (PC2 ؊/؊ ), responsible for the conversion of proglucagon into glucagon, while the second line consists of mice with a global deletion of the glucagon receptor (Gcgr ؊/؊ ). We demonstrate that nestin is transiently expressed by acinar cells and by insulin and glucagon cells of islets of both lines of mice. In addition, the lack of glucagon signaling increased nestin mRNA levels in pancreas of mutant embryos and adult mice. We conclude that nestin؉ cells located in the pancreatic primordium generate the cells of the endocrine and exocrine lineages. Furthermore, our results suggest that nestin expression is regulated by glucagon signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Kedees¹,

Guz²,

Vuguin³

et al. 2007

Developmental Dynamics

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show abstract

“…In addition to Pa 6, Pa 4 is expressed in beta cells [13]. Although Pa 6 is expressed in all four types of islet cells and in other organs including eyes and the central nervous system [6,7], Pa 4 is specifically expressed in beta cells [13]. The Pa 6 protein binds to a common element in the glucagon, insulin, and somatostatin promoters, and transactivates the glucagon and insulin promoters [8].…”

Section: Discussionmentioning

confidence: 99%

Diabetes and pancreatic tumours in transgenic mice expressing Pa × 6

et al. 2000

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“…14,15 In particular, PAX4 and PAX6 are essential for development of pancreatic islet cells as well as endocrine cell development in the small intestine and stomach. [14][15][16] Although PAX8 has been most studied in renal tissues and neoplasms, 17 it has been recently recognized as a useful marker in distinguishing ovarian from breast carcinoma, 18 and as a marker of thyroid epithelial neoplasms. 19 Moreover, our group recently observed intense PAX8 reactivity in pancreatic neuroendocrine tumors and normal pancreatic islets in a large tissue microarray evaluation of PAX8 immunoreactivity of non-renal tissues/neoplasms.…”

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confidence: 99%

PAX8 expression reliably distinguishes pancreatic well-differentiated neuroendocrine tumors from ileal and pulmonary well-differentiated neuroendocrine tumors and pancreatic acinar cell carcinoma

et al. 2011

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PAX (paired box) genes encode a family of transcription factors that regulate organogenesis in a variety of organs. Very little is known about the role of PAX8 in endocrine cell development and the expression of PAX8 in neuroendocrine tumors. The purpose of this study was to analyze PAX8 immunohistochemical expression in gastroenteropancreatic and pulmonary well-differentiated neuroendocrine tumors to determine whether PAX8 can reliably distinguish pancreatic neuroendocrine tumors from neuroendocrine tumors of other anatomic sites and other pancreatic non-ductal neoplasms. In total, 221 well-differentiated neuroendocrine tumors were evaluated: 174 primary neuroendocrine tumors (66 pancreatic, 31 ileal, 21 pulmonary, 20 gastric, 17 rectal, 11 appendiceal, and 8 duodenal) and 47 neuroendocrine tumors metastatic to the liver (31 pancreatic, 11 ileal, 2 pulmonary, 2 duodenal, and 1 rectal). Fifteen solid-pseudopapillary neoplasms and six acinar cell carcinomas of the pancreas were also evaluated. PAX8 was positive in 49/66 (74%) primary pancreatic neuroendocrine tumors. PAX8 expression did not correlate with World Health Organization categorization, grade, size, functional status, or the presence of liver or lymph node metastasis. PAX8 expression was identified in 0/31 (0%) ileal, 0/21 (0%) pulmonary, 2/20 (10%) gastric, 5/17 (29%) rectal, 1/11 (9%) appendiceal, and 6/8 (75%) duodenal neuroendocrine tumors. PAX8 was positive in 4/15 (27%) solid-pseudopapillary neoplasms of the pancreas, whereas all acinar cell carcinomas (0/6) lacked immunoreactivity. Among liver metastases, only pancreatic neuroendocrine tumors (20/31, 65%) were PAX8 positive, whereas no cases of ileal (0/11), pulmonary (0/2), duodenal (0/2), and rectal (0/1) neuroendocrine tumor metastases were PAX8 positive. PAX8 is expressed in primary and metastatic pancreatic well-differentiated neuroendocrine tumors, and its expression can reliably distinguish pancreatic from ileal and pulmonary well-differentiated neuroendocrine tumors. Duodenal neuroendocrine tumors and a subset of rectal, gastric, and appendiceal neuroendocrine tumors may also express PAX8. PAX8 expression can distinguish pancreatic neuroendocrine tumors from acinar cell carcinomas, but its utility in distinguishing neuroendocrine tumors from solid-pseudopapillary neoplasms is limited.

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Pax-QNR/Pax-6, a paired box- and homeobox-containing gene expressed in neurons, is also expressed in pancreatic endocrine cells.

Cited by 53 publications

References 0 publications

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Diabetes and pancreatic tumours in transgenic mice expressing Pa × 6

PAX8 expression reliably distinguishes pancreatic well-differentiated neuroendocrine tumors from ileal and pulmonary well-differentiated neuroendocrine tumors and pancreatic acinar cell carcinoma

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