Pax proteins in embryogenesis and their role in nervous system development

Namm, Aimar; Arend, Andres; Aunapuu, Marina

doi:10.12697/poa.2013.22.14

Cited by 1 publication

(2 citation statements)

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“…Transcription factor binding site enrichment and in silico drug screeen for neural regeneration AP-1 and Pax2 are enriched as the network regulators via transcription factor binding site enrichment analysis using a web-based tool named Enrichr [35]. Pax2 are expressed in populations of nerve cells within the developing forebrain, midbrain, hindbrain and spinal cord [36,37]. During CNS development, Pax2 have substantial roles in neural differentiation and regional specification, which also indicates its potential implications in neural regeneration [36,38].…”

Section: Closely Connected Subnetwork Obtained By Hotnet2mentioning

confidence: 99%

“…Pax2 are expressed in populations of nerve cells within the developing forebrain, midbrain, hindbrain and spinal cord [36,37]. During CNS development, Pax2 have substantial roles in neural differentiation and regional specification, which also indicates its potential implications in neural regeneration [36,38]. More importantly, AP-1 has been widely recognized as a major effector in neural regeneration.…”

Section: Closely Connected Subnetwork Obtained By Hotnet2mentioning

confidence: 99%

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Subnetwork identification and chemical modulation for neural regeneration: A study combining network guided forest and heat diffusion model

Wang

Zhu

et al. 2018

Quant. Biol.

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Background: The induction of neural regeneration is vital to the repair of spinal cord injury (SCI). While compared with peripheral nervous system (PNS), the regenerative capacity of the central nervous system (CNS) is extremely limited. This indicates that modulating the molecular pathways underlying PNS repair may lead to the discovery of potential treatment for CNS injury. Methods: Based on the gene expression profiles of dorsal root ganglion (DRG) after a sciatic nerve injury, we utilized network guided forest (NGF) to rank genes in terms of their capacity of distinguishing injured DRG from shamoperated controls. Gene importance scores deriving from NGF were used as initial heat in a heat diffusion model (HotNet2) to infer the subnetworks underlying neural regeneration in the DRG. After potential regulators of the subnetworks were found through Connectivity Map (cMap), candidate compounds were experimentally evaluated for their capacity to regenerate the damaged neurons. Results: Gene ontology analysis of the subnetworks revealed ubiquinone biosynthetic process is crucial for neural regeneration. Moreover, almost half of the genes in these subnetworks are found to be related to neural regeneration via text mining. After screening compounds that are likely to modulate gene expressions of the subnetworks, three compounds were selected for the experiment. Of them, trichostatin A, a histone deacetylase inhibitor, was validated to enhance neurite outgrowth in vivo via an optic nerve crush mouse model. Conclusions: Our study identified subnetworks underlying neural regeneration, and validated a compound can promote neurite outgrowth by modulating these subnetworks. This work also suggests an alternative approach for drug repositioning that can be easily extended to other disease phenotypes.Author summary: Compared with peripheral nervous system, the regenerative capacity of the central nervous system is extremely limited. Thus, it may lead to the discovery of treatment for central nerve injury by modulating gene networks underlying peripheral nerve injury. Based on the gene expression profiles of dorsal root ganglion after a sciatic nerve injury, we defined gene networks underlying peripheral nerve injury. After screening compounds that were likely to modulate gene expressions of the networks, trichostatin A, a histone deacetylase inhibitor, was validated to enhance neurite outgrowth in vivo via an optic nerve crush mouse model. † These authors contributed equally to this work.

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