Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research

Kent, Robyn S; Briggs, Emma; Colon, Beatrice L.; Álvarez, Catalina; Pereira, Sara Silva; Niz, Mariana De

doi:10.3389/fcimb.2022.900878

Cited by 3 publications

(1 citation statement)

References 463 publications

(559 reference statements)

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“…Integrating multi-omics data, including proteomics, genomics, and transcriptomics, is crucial for understanding parasite biology and identifying new drug and vaccine targets. Effective data management strategies are essential to harness the potential of "big data" in parasitology [176].…”

Section: Proteomics Approach In Drug Discovery Effortsmentioning

confidence: 99%

Proteomics Applications in Toxoplasma gondii: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery

Deng,

Vanagas,

Alonso

et al. 2023

Pathogens

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Toxoplasma gondii, a protozoan parasite with the ability to infect various warm-blooded vertebrates, including humans, is the causative agent of toxoplasmosis. This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially affecting the fetus through congenital transmission. A comprehensive understanding of the intricate molecular interactions between T. gondii and its host is pivotal for the development of effective therapeutic strategies. This review emphasizes the crucial role of proteomics in T. gondii research, with a specific focus on host–parasite interactions, post-translational modifications (PTMs), PTM crosstalk, and ongoing efforts in drug discovery. Additionally, we provide an overview of recent advancements in proteomics techniques, encompassing interactome sample preparation methods such as BioID (BirA*-mediated proximity-dependent biotin identification), APEX (ascorbate peroxidase-mediated proximity labeling), and Y2H (yeast two hybrid), as well as various proteomics approaches, including single-cell analysis, DIA (data-independent acquisition), targeted, top-down, and plasma proteomics. Furthermore, we discuss bioinformatics and the integration of proteomics with other omics technologies, highlighting its potential in unraveling the intricate mechanisms of T. gondii pathogenesis and identifying novel therapeutic targets.

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Section: Proteomics Approach In Drug Discovery Effortsmentioning

confidence: 99%

Proteomics Applications in Toxoplasma gondii: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery

Deng,

Vanagas,

Alonso

et al. 2023

Pathogens

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The intersection of host in vivo metabolism and immune responses to infection with kinetoplastid and apicomplexan parasites

Ewald,

Nasuhidehnavi,

Feng

et al. 2024

Microbiol Mol Biol Rev

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SUMMARY Protozoan parasite infection dramatically alters host metabolism, driven by immunological demand and parasite manipulation strategies. Immunometabolic checkpoints are often exploited by kinetoplastid and protozoan parasites to establish chronic infection, which can significantly impair host metabolic homeostasis. The recent growth of tools to analyze metabolism is expanding our understanding of these questions. Here, we review and contrast host metabolic alterations that occur in vivo during infection with Leishmania , trypanosomes, Toxoplasma , Plasmodium, and Cryptosporidium . Although genetically divergent, there are commonalities among these pathogens in terms of metabolic needs, induction of the type I immune responses required for clearance, and the potential for sustained host metabolic dysbiosis. Comparing these pathogens provides an opportunity to explore how transmission strategy, nutritional demand, and host cell and tissue tropism drive similarities and unique aspects in host response and infection outcome and to design new strategies to treat disease.

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Proteomics Applications in <em>Toxoplasma gondii</em>: Unveiling the Host-Parasite Interactions and Therapeutic Target Discovery

Deng,

Vanagas,

Angel

2023

Preprint

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Toxoplasma gondii, a protozoan parasite with the ability to infect various warm-blooded vertebrates, including humans, is the causative agent of toxoplasmosis. This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially affecting the fetus through congenital transmission. A comprehensive understanding of the intricate molecular interactions between T. gondii and its host is pivotal for the development of effective therapeutic strategies. This review emphasizes the crucial role of proteomics in T. gondii research, with a specific focus on host-parasite interactions, post-translational modifications (PTMs), PTM crosstalk, and ongoing efforts in drug discovery. Additionally, we provide an overview of recent advancements in proteomics techniques, encompassing interactome sample preparation methods such as BioID, APEX, and Y2H, as well as various proteomics approaches, including single-cell analysis, DIA, targeted, top-down, and plasma proteomics. Furthermore, we discuss the integration of proteomics with other omics technologies, highlighting its potential in unraveling the intricate mechanisms of T. gondii pathogenesis and identifying novel therapeutic targets.

show abstract

Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research

Cited by 3 publications

References 463 publications

Proteomics Applications in Toxoplasma gondii: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery

Proteomics Applications in Toxoplasma gondii: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery

The intersection of host in vivo metabolism and immune responses to infection with kinetoplastid and apicomplexan parasites

Proteomics Applications in <em>Toxoplasma gondii</em>: Unveiling the Host-Parasite Interactions and Therapeutic Target Discovery

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