Patterns of urinary enzyme excretion in healthy subjects

Werner, Mario; Heilbron, David C.; Maruhn, D; Atoba, Mudasiru

doi:10.1016/0009-8981(70)90014-8

Cited by 32 publications

(13 citation statements)

References 16 publications

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“…These findings seem to be in keeping with the report by Werner et al [38] about the absence of circadian rhythms in the urinary excretion of various enzymes. Actually, the possibility of circa dian rhythms, already found by Peters et al [28] as regards the excretion of alanyl-peptidhydrolase, cannot be excluded with certainty by our data.…”

Section: Discussionsupporting

confidence: 82%

Reference Ranges and Methodological Aspects in the Urinary Measuring of Lysozyme, Malate-Dehydrogenase, γ-Glutamyltransferase and a-Glucosidase

Amodio¹,

Bazzerla²,

Malatesta³

et al. 1985

Enzyme

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The urinary excretions of lysozyme (LYS), malate-dehydrogenase (MAD), y-glutamyltransferase (GGT) and a-glucosidase (AGL) were measured in a group of normal subjects in basic conditions, during forced diuresis, at different hours of the day and with urinary collection periods of different lengths. The results have been expressed in the principal ways used in clinical practice. The best way to express the excretions of GGT and AGL was with the fractional excretions. For LYS and MAD, the fractional clearances appeared to be theoretically valuable. They were not significantly influenced by sex or by the different urinary collections. Forced diuresis caused a significant scattering of enzymuria.

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Section: Discussionsupporting

confidence: 82%

Reference Ranges and Methodological Aspects in the Urinary Measuring of Lysozyme, Malate-Dehydrogenase, γ-Glutamyltransferase and a-Glucosidase

Amodio¹,

Bazzerla²,

Malatesta³

et al. 1985

Enzyme

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“…For albumin, measured in urines of normal persons, Viberti et al (27,28) found ä large intra-individual Variation, which was pnjy slightly less when related to creatinine. Calculation of urinary enzyme activities, in relation to creatinine reduced the intra-individual variabilities (29,30,31). Our present results confirm and extend these pbservations.…”

Section: Biological Variation Of Urinary Proteinsmentioning

confidence: 99%

“…With regard to urine protein and enzyme activity determination, no uniform recommendations exist in the literature (19,27,29,35,37).…”

Section: Selection Of Samplementioning

confidence: 99%

A Diagnostic Programme for Quantitative Analysis of Proteinuria

Hofmann

Guder²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:A spectrum of quantitative methods was adapted to the Kone Specific Analyser for the purpose of recognizing, quantifying and differentiating various forms of proteinuria. Total protein, IgG, albumin and oci-microglobulin (measured by turbidimetry), N-acetyl-ß-.D-glucosaminidase activity and creatinine (measured photometrically), were measured in undiluted urine; in addition a r microglobulin was measured in serum.Within and between run predsion, accuracy and linearity of the turbidimetric methods were in good agreement with nephelometric procedures. All turbidimetric methods exhibited a correlation coefficient r > 0.98 when compared with the radial immunodiffusion procedure äs reference method. Total protein measured turbidimetrically with the Kone Specific Analyser was in good agreement with the manual biuret procedure.The low detection limits and linearities allowed quantification of urine analytes from the lower ränge of normals up to ten times the upper limit of normals.The measured analytes exhibited stability in urine at pH 4-8 over at least seven days at 4 -6 °C and -20 °C. Only IgG showed a significant loss (up to 30 percent), when measured after storage at -20 °C.Quantities per mol creatinine showed significantly lower intra-individual and inter-individual variability than quantities per liter. In 31 normal persons, the intraindividual Variation was lowest for N-acetyl-ß-Z>-glucosaminidase activity (13%) and highest for total protein (33%), when measured in the second morning urine on 5 consecutive days.When related to creatinine, resülts obtäined in the second morning urine showed no significant differences from those in 24 h urine, except for aj-microglobulin which gave lower values in 24 h urines.The upper normal limits, calculated äs the 95% ranges, were determined from 154 urines of 31 individuals. Nearly all analytes showed an asymmetric distribution. Because of a wide tailing of the upper limit, preliminary üpper normal limits were set aböve this ränge:Total protein: 7.9 g/mol creatinine (70 mg/g creatinine) IgG:1.13 g/mol creatinine (10 mg/g creatinine) Albumin:2.26 g/mol creatinine (20 mg/g creatinine) ocrMicroglobulin: " 1.58 g/mol creatinine (14 mg/g creatinine) N^Acetyl-ß-jD-glücosaminidäse: 0.56 kU/mol creatinine (5 U/g creatinine).Application of the newly adapted programme to unselected urines sent for urine analysis revealed a threefold incfease in the propörtion of resülts outside the normal ranges, compared with the routinely used protein test strip procedure. All additional positive urines exhibited either signs of glomerular or tubular proteinuria. Determination of albumin or N-acetyl-ß-jD-glucosaminidase excretion was sufficient to detect these additional cases.

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“…The highest urinary excretion rates of arylsulphatase A, -glucosidase, -galactosidase and -glucuronidase, during 24 hour collection, were found betwen 6 00 -9 00 AM, and the lowest between 1 00 -9 00 PM. Therefore, collection of urine for 3-hours between 6 00 -9 00 AM was recommended for determination urinary enzymes (Werner et al, 1970). Other authors (Jung et al, 1992) recommend 2-hour collection of urine, i.e.…”

Section: Stabilization Of Fbp-16 and Hex Activities In Human Urinementioning

confidence: 99%

“…Kotanko P. and Pfaller W. (Kotanko et al, 1997;Pfaller et al, 1994) recommend collection of urine from second morning midstream, as an appropriate specimen for analysis of urinary enzymes. The first portion of urine voided after night, should be definitely avoided, as storage of enzymes in urine during the night, may cause enzyme denaturation (Maruhn, 1983;Werner et al, 1970). Actually, urinary enzymes are commonly determined in urine of the second morning midstream collection, and calculated on gram or mole of excreted creatinine in this urine (Kotanko et al, 1997).…”

Section: Stabilization Of Fbp-16 and Hex Activities In Human Urinementioning

confidence: 99%

Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation - Literature Review

Kępka¹,

Szajda²,

Waszkiewicz³

et al. 2011

Kidney Transplantation - New Perspectives

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Patterns of urinary enzyme excretion in healthy subjects

Cited by 32 publications

References 16 publications

Reference Ranges and Methodological Aspects in the Urinary Measuring of Lysozyme, Malate-Dehydrogenase, γ-Glutamyltransferase and a-Glucosidase

Reference Ranges and Methodological Aspects in the Urinary Measuring of Lysozyme, Malate-Dehydrogenase, γ-Glutamyltransferase and a-Glucosidase

A Diagnostic Programme for Quantitative Analysis of Proteinuria

Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation - Literature Review

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