Patterns of Trichothecene Production, Genetic Variability, and Virulence to Wheat of <i>Fusarium</i> <i>graminearum</i> from Smallholder Farms in Nepal

Desjardins, Anne E.; Jarosz, A. M.; Plattner, Ronald D.; Alexander, Nancy J.; Brown, Daren W.; Jurgenson, James E.

doi:10.1021/jf040181e

Cited by 34 publications

(19 citation statements)

References 34 publications

(75 reference statements)

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“…1). The physical locations of TRI5, TRI15, and the SCAR marker were consistent with the previous linkage analyses (1,11,19). The structural genes MGV1, PHO, RED, and TRI101 also were located in expected positions on the physical map.…”

Section: Discussionsupporting

confidence: 88%

“…These genetic conclusions were confirmed by Lee et al (25), who showed that TRI13 determines which toxin type is produced and that this locus is located in the trichothecene cluster near TRI5. Alexander et al (1) used the map to locate Tri15 on LG 2, and Desjardins et al (11) used the map to locate a diagnostic SCAR marker locus on LG 5. The genetic map also was used to map loci for pathogenicity and aggressiveness of G. zeae toward wheat (8).…”

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confidence: 99%

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Alignment of Genetic and Physical Maps of Gibberella zeae

Lee

Jurgenson

Leslie

et al. 2008

Appl Environ Microbiol

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We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) using seven structural genes and 108 sequenced amplified fragment length polymorphism markers. Several linkage groups were combined based on the alignments, the nine original linkage groups were reduced to six groups, and the total size of the genetic map was reduced from 1,286 to 1,140 centimorgans. Nine supercontigs, comprising 99.2% of the genomic sequence assembly, were anchored to the genetic map. Eight markers (four markers from each parent) were not found in the genome assembly, and four of these markers were closely linked, suggesting that >150 kb of DNA sequence is missing from the PH-1 genome assembly. The alignments of the linkage groups and supercontigs yielded four independent sets, which is consistent with the four chromosomes reported for this fungus. Two proposed heterozygous inversions were confirmed by the alignments; otherwise, the colinearity of the genetic and physical maps was high. Two of four regions with segregation distortion were explained by the two selectable markers employed in making the cross. The average recombination rates for each chromosome were similar to those previously reported for G. zeae. Despite an inferred history of genetic isolation of lineage 6 and lineage 7, the chromosomes of these lineages remain homologous and are capable of recombination along their entire lengths, even within the inversions. This genetic map can now be used in conjunction with the physical sequence to study phenotypes (e.g., fertility and fitness) and genetic features (e.g., centromeres and recombination frequency) that do not have a known molecular signature in the genome.

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Section: Discussionsupporting

confidence: 88%

mentioning

confidence: 99%

Alignment of Genetic and Physical Maps of Gibberella zeae

Lee

Jurgenson

Leslie

et al. 2008

Appl Environ Microbiol

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“…In particular the relative frequency of DON and NIV is of concern because recent reports suggest that strains of ND 4618 13 IIN ND ND ND ND ND ND 4624 14 IIS ND ND ND ND ND ND 4625 14 IIS ND ND ND ND ND ND 4627 14 IIS ND ND ND ND ND ND 4628 14 IIS ND ND ND ND ND ND 4631 9 IIS ND ND ND ND ND ND 4633 9 IIS ND ND ND ND ND ND 4605 7 IV ND ND ND ND ND ND the F. graminearum complex that produce NIV may be more aggressive towards corn but less aggressive to wheat than DON producing strains. These results indicate that there may be important consequences for the fitness and aggressiveness of FHB pathogens of different chemotype on particular hosts (3,7,11). Further studies will be necessary in order to characterize the genetic diversity, chemotype and virulence of F. graminearum populations from Argentina, a country where this species is widespread and wheat is cultivated under different agro-meteorological conditions.…”

Section: Discussionmentioning

confidence: 99%

Natural occurrence of nivalenol and mycotoxigenic potential of Fusarium graminearum strains in wheat affected by head blight in Argentina

Pinto¹,

Terminiello²,

Basílico³

et al. 2008

Braz. J. Microbiol.

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The principal agents of Fusarium head blight in the main cropping area of Argentina were investigated in heavily infected samples. The ability of the isolates to produce trichothecenes was determined by GC and HPLC. Fusarium graminearum was the predominant species and of 33 isolates, 10 produced deoxinivalenol (DON) (0.1-29 mg kg ), 10 produced Fusarenone X (0.1-2.4 mg kg -1 ) and 7 produced zearalenone (0.1-0.6 mg kg -1 ). These results suggest that F. graminearum strains isolated from the wheat growing regions in Argentina belong to DON chemotype. Although some strains produced both deoxinivalenol and nivalenol, nivalenol was produced in lower levels. The natural occurrence of nivalenol in wheat affected by head-blight collected in the main production area during two years (2001)(2002) was also determined. From 19 samples 13 were contaminated with deoxinivalenol in a range of 0.3 to 70 mg kg -1 and 2 samples with both deoxinivalenol (7.5 and 6.7 mg kg -1 ) and nivalenol (0.05 and 0.1 mg kg -1 ), respectively. This is the first report of natural occurrence of nivalenol in wheat cultivate in Argentina.

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“…In planta, trichothecenes are known to act by blocking the synthesis of proteins and favor pathogen colonization in host tissue (Desjardins et al, 1993;Desjardins et al, 1996). Moreover, DON is recognized as a virulence factor during pathogenesis, with more symptoms being associated with higher DON production, whereas NIV in wheat is produced in smaller quantities than DON (Desjardins et al, 2004).…”

Section: Central-spikelet Inoculation Assaymentioning

confidence: 99%

Common resistance to Fusarium head blight in Brazilian wheat cultivars

Mendes

Ponte

Feltrin

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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Mycotoxin levels in Fusarium head blight (FHB) infections can be difficult to quantify.The relationship between mycotoxin and disease is not consistent and it is not clear if wheatpathogen interaction is of significance in regions where more than one Fusarium species with distinct trichothecene production ability co-exists. This study aimed to investigate whether a set of eight Brazilian wheat genotypes, varying in resistance according to classification by the breeder, exhibit a common or differential resistance to Fusarium graminearum (Fgra) (deoxynivalenolproducing) and Fusarium meridionale (nivalenol-producing) (Fmer) using full-spike and central spikelet inoculation (type II resistance). Fgra was generally more aggressive than Fmer based on the percentage of diseased spikes (99 and 84 %, respectively) and number of diseased spikelets (mean 2.8 and 2.0, respectively) below the central spikelet. The genotype-pathogen species interaction was not significant, but there were differences between the genotypes, with BRS 194 and BRS 327 being the least and most resistant, respectively, based on severity ratings. The incidence of Fusarium-damaged kernel (FDK, %) was not affected by species, but two genotypes , respectively, suggesting that Fgra is a more potent producer of trichothecene. Our data confirm prior resistance classifications by the breeders and suggest that the use of a single highly aggressive Fgra isolate may be sufficient for effective screening for FHB resistance. However, further studies are needed to elucidate the accumulation of resistance to trichothecene.

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Patterns of Trichothecene Production, Genetic Variability, and Virulence to Wheat of Fusarium graminearum from Smallholder Farms in Nepal

Cited by 34 publications

References 34 publications

Alignment of Genetic and Physical Maps of Gibberella zeae

Alignment of Genetic and Physical Maps of Gibberella zeae

Natural occurrence of nivalenol and mycotoxigenic potential of Fusarium graminearum strains in wheat affected by head blight in Argentina

Common resistance to Fusarium head blight in Brazilian wheat cultivars

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