Patterns of sperm use by twice‐mated female Queensland fruit flies

Abstract

Multiple mating by females, polyandry, is common in insects, including in tephritid fruit flies. Female insects that remate commonly store sperm of multiple males. How the sperm of different males contribute to paternity is an important element of sexual selection. Sexual behavior and reproduction of the Queensland fruit fly (Qfly), Bactrocera tryoni, has been extensively investigated both in relation to understanding this economically important species’ reproductive biology and in relation to implications for Sterile Insect Technique (SIT), whereby sterile flies are released to constrain reproduction of pest populations. Despite numerous studies of pre‐ and postcopulatory sexual selection in Qfly, there have been no direct studies of paternity patterns in polyandrous female Qflies. We used two morphologically distinguishable lines to investigate patterns of sperm use in Qfly. The two lines showed comparable mating performance evidenced by similar mating and remating frequency, copula duration, and proportion of second mate paternity (P2) between reciprocal crosses. The mechanism of sperm usage, with P2 close to 0.5 immediately after the second mating followed by gradual decrease of P2 as females aged, is most consistent with stratification or repositioning of sperm. Patterns observed in the present study are compared with the available information from other tephritid fruit flies, and are discussed in relation to this species’ reproductive biology, known patterns of sperm storage, and SIT.

Postcopulatory Behavior of Tephritid Flies

“…For the tephritids studied to date, there seems to be differential degrees of sperm mixing when a female remates, with initial stratification, then mixing, leading to predominance of the first male in B. tryoni and predominance of the second male in R. pomonella, A. suspensa, C. capitata, and A. ludens (41, 99,133,136,161). In contrast to C. capitata, in A. ludens, predominance of the second male does not decrease with time (133,161).…”