Patterns of puffing activity and chromosomal polymorphism in Drosophila subobscura

Frutos, Rosa de; Latorre, Amparo; Pascual, Luis Alberto Malvárez

doi:10.1007/bf00056028

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…Most available studies are focused on the effects of inbreeding on fitness components (Torroja, 1964;Kosuda, 1972;Wallace & Madden, 1965;Latter & Mulley, 1995; and on quantitative traits (Tantawy & Reeve, 1956;Lints, 1961). In D. subobscura, the effect of inbreeding on the rate of development, fertility (Hollingsworth & Maynard Smith, 1955), and the pattern of puffing activity in relation to chromosomal polymorphism (De Frutos, Latorre & Pascual, 1984) has also been studied. To our knowledge, allozyme loci have never been used in this species as genetic markers to analyze the fixation rates and the expected heterozygosity after a given number of generations of sib mating.…”

Section: Introductionmentioning

confidence: 99%

Analysis of inbreeding in a colonizing population of Drosophila subobscura

1996

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An analysis of the effects of inbreeding on the genetic structure of a colonizing population of Drosophila subobscura has been carried out. Species of Drosophila, particularly D. subobscura, may have lethal alleles associated with chromosomal inversions and our aim was to assess the extent to which the genome is balanced in this way. The frequencies of chromosomal inversions were compared between a large population and a set of 72 lines that were maintained by brother-sister mating for 10 generations. Fisher's matrix method was used to calculate the expected homozygosity in these inbred lines for 5 allozyme loci (Aph, Hk-1, Lap, Odh, and Pept-1) used as markers of large chromosomal segments. Furthermore, the expected rates of fixation corresponding to these allozyme loci were also calculated. The results show that the amount of homozygosis observed did not differ significantly from expectations (with the corresponding loss of lines as a consequence of the reduction in viability). However, two deviations from strict neutrality were observed: there was a heterozygote excess at the Lap locus, and the frequency of the O5 inversion (always associated with a lethal gene in colonizing populations) was higher than expected.

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Section: Introductionmentioning

confidence: 99%

Analysis of inbreeding in a colonizing population of Drosophila subobscura

1996

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“…A weakening in gene activity has already been described in the literature about an inbred line of D. melanogaster (Lychev, 1965). De Frutos et al (1984 observed a general depression of the puffing activity m a D. subobscura laboratory strain (K228) subjected to prolonged inbreeding, with respect to other D. subobscura stocks.…”

Section: Biological Distancementioning

confidence: 65%

Differences in gene activity in a Drosophila species cluster belonging to the Obscura group

1993

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The polytene chromosome puffing pattern of Drosophila madeirensis was established and compared with those of the related species D. subobscura and D. guanche. A total of 145 loci, active in some of the 12 developmental stages analysed, were described, 38 of which were found to form the puffing pattern characteristic to this species. Taking into account the number of puffs as well as the mean puff expression, D. madeirensis shows a similar activity level to D. guanche, both species being less active than D. subobscura. The low gene activity of D. madeirensis and D.guanche was explained as a consequence of their ecological characteristics.

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“…The patterns ofpuffing activity in the polytene chromosomes represent one morphological expression of gene activity in these chromosomes (Beerman, 1952) and hence may be used for obtaining information about differences in gene activity between chromosomes with the same or different chromosomal arrangements. Puffing patterns of D. subobscura have been well established in our laboratory (de Frutos andLatorre, 1982a, 1982b;de Frutos et al, 1984de Frutos et al, , 1987Pascual et al, 1985).…”

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confidence: 81%

“…These 160 puffs show variable levels of activity. Twenty-seven of them reach a frequency higher than 75% across all strains in at least one of the developmental stages and have been considered to represent the characteristic puffing pattern of D. subobscura (see de Frutos et al [1984] for a more detailed explanation). Ofthe rest ofthe puffs, many reach a medium frequency (between 25% and 75%).…”

Section: Methodsmentioning

confidence: 99%

PATTERNS OF PUFFING ACTIVITY AND CHROMOSOMAL POLYMORPHISM IN DROSOPHILA SUBOBSCURA . IV. EFFECT OF INVERSIONS ON GENE EXPRESSION

1988

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We have observed that, contrary to a common assumption, the puffing patterns manifest in the salivary chromosomes of Drosophila subobscura are modified by chromosomal inversions as well as by genic content. An inversion effect is apparent in the E and A chromosomes of five strains coming from four different natural populations. An effect due to the geographical location of the populations is also detected in the J and O chromosomes. The chromosomal and geographic effects are distinguishable but not contradictory. Indeed, a statistical test using the DK2 coefficient of distance shows that, for a given chromosomal arrangement, strains of different geographic origin exhibit puffing patterns significantly different; these patterns are, however, more similar to each other than they are to those of strains carrying different chromosomal arrangements of the same chromosome.

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Patterns of puffing activity and chromosomal polymorphism in Drosophila subobscura

Cited by 12 publications

References 15 publications

Analysis of inbreeding in a colonizing population of Drosophila subobscura

Analysis of inbreeding in a colonizing population of Drosophila subobscura

Differences in gene activity in a Drosophila species cluster belonging to the Obscura group

PATTERNS OF PUFFING ACTIVITY AND CHROMOSOMAL POLYMORPHISM IN DROSOPHILA SUBOBSCURA . IV. EFFECT OF INVERSIONS ON GENE EXPRESSION

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