Patterns of protein synthesis in endometrial tissues from ovariectomized rats treated with oestradiol and progesterone

Abstract

Groups of ovariectomized rats were taken as controls or given hormonal treatment mimicking the successive steps in the sequence of ovarian secretions leading to implantation. Total endometrium or separated epithelium and stroma were incubated in vitro with [35S]methionine. Dissolved proteins were submitted to two-dimensional polyacrylamide gel electrophoresis (pH range 5-7), followed by autoradiography. Priming with oestradiol (2 days) and subsequent treatment with progesterone (3 days) enhanced the synthesis of 12 and 14 polypeptides, respectively, which are specific for each of these treatments. Progesterone also suppressed the production of 10 oestrogen-dependent proteins both in the epithelium and the stroma. When an oestrogen-progesterone-oestrogen treatment was given, synthesis of all but 4 of the progesterone-induced polypeptides in the epithelium was inhibited while 5 of the proteins abolished by progesterone in this tissue compartment reappeared. These results are compatible with a mechanism of implantation acting at the epithelial level by lifting of intrauterine inhibition and stimulation by embryotrophic substances.

Estrous-Cycle-Related Changes in the Expression of Mouse Endometrial and Oviductal Glycoproteins

“…Nevertheless, some of the bands detected in the lectin overlays of SDS-PAGE-separated cytoplasmic glycoproteins probably correspond to pre viously described secretory proteins. Thus the 25-kD diestrus-related RCA-I-reactive endometrial mouse glycopro tein could be equivalent to the 30-kD rat protein induced by estrogen and progesterone treatment [20]. The 138-kD estradiol-induced guinea pig [11] secretory protein could be equivalent to the 135-kD glycoprotein from the present study.…”

Changes related to the oestrous cycle in the expression of endometrial and oviductal proteins of mice

“…The comparison of our data with those from one-dimensional SDS-PAGE (Umapathysivam & Jones, 1978) was not feasible because of the differences between the one-and two-dimensional gel electrophoresis. Proteins numbered 12 and 13 from our study have the same properties as proteins labelled 12 and 19 in two-dimensional SDS-PAGE of rat uteri by Lejeune et al (1985). Our protein number 4 (Mr 35, pi 5-60), which appears in dioestrus, is probably equivalent to the rat protein number 16 (Lejeune et al, 1985), which is induced by oestrogen and progesterone in epithelial cells.…”

“…Proteins numbered 12 and 13 from our study have the same properties as proteins labelled 12 and 19 in two-dimensional SDS-PAGE of rat uteri by Lejeune et al (1985). Our protein number 4 (Mr 35, pi 5-60), which appears in dioestrus, is probably equivalent to the rat protein number 16 (Lejeune et al, 1985), which is induced by oestrogen and progesterone in epithelial cells. Furthermore, our protein number 26 probably corresponds to the 115 kDa protein described in the rat endometrium by Jacobs & Lyttle (1987).…”

Expression of carbohydrate antigens in the rat uterus during early pregnancy and after ovariectomy and steroid replacement

“…Although a number of endometrial epithelial (glyco)proteins are stimulated by oestrogen (Kuivanen and De Sombre, 1985;Lejeune et al, 1985;Anderson et al, 1986), there are few molecular markers of specific phases in the endometrial cycle. In ovariectomized rats, three endometrial epithelial proteins detected in the pre-receptive period disappeared within 12 h of injection with nidatory oestrogen (receptive period), but no new proteins could be detected.…”